Results 71 to 80 of about 4,623 (199)

Comparison of transcriptional profiles of Clostridium thermocellum grown on cellobiose and pretreated yellow poplar using RNA-Seq

Frontiers in Microbiology, 2014
The anaerobic, thermophilic bacterium, Clostridium thermocellum, secretes multi-protein enzyme complexes, termed cellulosomes, which synergistically interact with the microbial cell surface and efficiently disassemble plant cell wall biomass.
Hui eWei, Yan eFu, Lauren eMagnusson, John O. Baker, Pin-Ching eManess, Qi eXu, Shihui eYang, Andrew eBowersox, Andrew eBowersox, Igor eBogorad, Wei eWang, Melvin P. Tucker, Michael E. Himmel, Shi-You eDing   +13 more
doaj   +1 more source

Efficient Biomass Fractionation via Organosolv for Sustainable Bioenergy Production: A Comprehensive Review

International Journal of Chemical Engineering, Volume 2025, Issue 1, 2025.
Biomass is a significant renewable feedstock for the generation of bioenergy and a gateway to a shift from fossil fuels to a renewable energy system. Efficient fractionation of biomass into the main components (cellulose, hemicellulose, and lignin) is still a problem due to its complexity.
Tesfaye Kassaw Bedru, Beteley Tekola Meshesha, Shegaw Ahmed Mohammed, Abayneh Getachew Demesa, Mani Jayakumar, Andreas Bück   +5 more
wiley   +1 more source

Structure of CBM4 fromClostridium thermocellumcellulase K [PDF]

Acta Crystallographica Section F Structural Biology and Crystallization Communications, 2011
Here, a 2.0 Å resolution X-ray structure of Clostridium thermocellum cellulase K family 4 carbohydrate-binding module (CelK CBM4) is reported. The resulting structure was refined to an R factor of 0.212 and an R(free) of 0.274. Structural analysis shows that this new structure is very similar to the previously solved structure of C.
Markus, Alahuhta, Yonghua, Luo, Shi You, Ding, Michael E, Himmel, Vladimir V, Lunin   +4 more
openaire   +2 more sources

Exploiting Archaeal/Thermostable Enzymes in Synthetic Chemistry: Back to the Future?

ChemCatChem, Volume 16, Issue 21, November 11, 2024.
Thermostable and (hyper)thermophilic enzymes offer important opportunities for innovative, stereoselective and sustainable chemistry transformations. Many of these are archaeal enzymes that have evolved to operate under extreme conditions, including at elevated temperature.
Gaurav P. Kudalkar, Virendra K. Tiwari, David B. Berkowitz   +2 more
wiley   +1 more source

Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase

Microbial Cell Factories, 2017
Background Pyruvate decarboxylase (PDC) is a well-known pathway for ethanol production, but has not been demonstrated for high titer ethanol production at temperatures above 50 °C. Result Here we examined the thermostability of eight PDCs.
Liang Tian, Skyler J. Perot, Shuen Hon, Jilai Zhou, Xiaoyu Liang, Jason T. Bouvier, Adam M. Guss, Daniel G. Olson, Lee R. Lynd   +8 more
doaj   +1 more source

Evolution of glucuronoxylan side chain variability in vascular plants and the compensatory adaptations of cell wall–degrading hydrolases

New Phytologist, Volume 244, Issue 3, Page 1024-1040, November 2024.
Summary Polysaccharide structural complexity not only influences cell wall strength and extensibility but also hinders pathogenic and biotechnological attempts to saccharify the wall. In certain species and tissues, glucuronic acid side groups on xylan exhibit arabinopyranose or galactose decorations whose genetic and evolutionary basis is completely ...
Li Yu, Louis F. L. Wilson, Oliver M. Terrett, Joel Wurman‐Rodrich, Jan J. Łyczakowski, Xiaolan Yu, Kristian B. R. M. Krogh, Paul Dupree   +7 more
wiley   +1 more source

Enhanced Enzyme Immobilization Using a Novel Agarose‐binding Tag Leads to Improved Flow Reactor Performance

ChemCatChem, Volume 16, Issue 19, October 7, 2024.
To enhance enzyme retention within agarose hydrogels, a carbohydrate‐binding module (MtCBM) derived from Microbulbifer thermotolerans thermostable β‐agarase I was utilized as a versatile agarose‐binding tag. MtCBM displayed heightened binding affinity towards unmelted agarose, and fusion proteins incorporating MtCBM exhibited improved retention within ...
Martin Peng, Matthias Franzreb, Annika J. Weber, Phillip Lemke, Christof M. Niemeyer, Kersten S. Rabe   +5 more
wiley   +1 more source

Regulation of Cellulase Formation in Clostridium thermocellum

Microbiology, 1985
Summary: True cellulase activity (i.e. degradation of crystalline cellulose) was markedly derepressed when cellobiose-grown cells were transferred to fructose or sorbitol, especially while the cells were adapting over many hours to these carbon sources. On the other hand, the long lag phase on glucose was not accompanied by derepression.
E. A. JOHNSON, F. BOUCHOT, A. L. DEMAIN
openaire   +1 more source

Chromogenic fusion proteins as alternative textiles dyes

Biotechnology and Bioengineering, Volume 121, Issue 9, Page 2820-2832, September 2024.
Abstract The widespread adoption of fast fashion has led to a significant waste problem associated with discarded textiles. Using proteins to color textiles can serve as a sustainable alternative to chemical dyes as well as reduce the demand for new raw materials.
Tyson Watkins, Kaylee Moffitt, Robert E. Speight, Laura Navone   +3 more
wiley   +1 more source

Biomass augmentation through thermochemical pretreatments greatly enhances digestion of switchgrass by Clostridium thermocellum

Biotechnology for Biofuels, 2018
Background The thermophilic anaerobic bacterium Clostridium thermocellum is a multifunctional ethanol producer, capable of both saccharification and fermentation, that is central to the consolidated bioprocessing (CBP) approach of converting ...
Ninad Kothari, Evert K. Holwerda, Charles M. Cai, Rajeev Kumar, Charles E. Wyman   +4 more
doaj   +1 more source