Results 41 to 50 of about 5,725 (156)

ClpAP proteolysis does not require rotation of the ClpA unfoldase relative to ClpP

eLife, 2020
AAA+ proteases perform regulated protein degradation in all kingdoms of life and consist of a hexameric AAA+ unfoldase/translocase in complex with a self-compartmentalized peptidase.
Sora Kim, Kristin L Zuromski, Tristan A Bell, Robert T Sauer, Tania A Baker   +4 more
doaj   +1 more source

The ClpXP protease and the ClpX unfoldase control virulence, cell division, and autolysis in Streptococcus pneumoniae

Microbiology Spectrum
In all living cells, molecular chaperones and ATP-dependent proteases are essential for protein homeostasis. The ClpXP protease is a chaperone-protease complex conserved between bacteria and mitochondria.
Viktor H. Mebus, Supradipta De, Larissa M. Busch, Manuela Gesell Salazar, Rabea Schlüter, Uwe Völker, Sven Hammerschmidt, Dorte Frees   +7 more
doaj   +1 more source

The Mycobacterium tuberculosis ClpP1P2 Protease Interacts Asymmetrically with Its ATPase Partners ClpX and ClpC1. [PDF]

PLoS ONE, 2015
Clp chaperone-proteases are cylindrical complexes built from ATP-dependent chaperonerings that stack onto a proteolytic ClpP double-ring core to carry out substrate protein degradation.Interaction of the ClpP particle with the chaperone is mediated by an
Julia Leodolter, Jannis Warweg, Eilika Weber-Ban   +2 more
doaj   +1 more source

α-Amino Diphenyl Phosphonates as Novel Inhibitors of Escherichia coli ClpP Protease [PDF]

Journal of Medicinal Chemistry, 2018
Increased Gram-negative bacteria resistance to antibiotics is becoming a global problem, and new classes of antibiotics with novel mechanisms of action are required. The caseinolytic protease subunit P (ClpP) is a serine protease conserved among bacteria that is considered as an interesting drug target. ClpP function is involved in protein turnover and
Carlos Moreno-Cinos, Elisa Sassetti, Irene G. Salado, Gesa Witt, Siham Benramdane, Laura Reinhardt, Cristina D. Cruz, Jurgen Joossens, Pieter Van der Veken, Heike Brötz-Oesterhelt, Päivi Tammela, Mathias Winterhalter, Philip Gribbon, Björn Windshügel, Koen Augustyns   +14 more
openaire   +3 more sources

Systematic Engineering of Proteases in Saccharopolyspora Spinosa Reveals Synergistic Enhancement of Spinosad Biosynthesis via Substrate Flux Optimization

Advanced Science, EarlyView.
ARTP mutagenesis yielded Saccharopolyspora spinosa mutant D184 with improved extracellular nitrogen utilization. An integrated workflow of protease genetic manipulation, multi‐omics, and rational synergy design pinpointed a pepP‐clpP‐htpX synergistic triangular combination.
Duo Jin, Wangqiong Chen, Danlu Yang, Qing Liu, Baolong Bai, Zirui Dai, Xirong Liu, Zirong Zhu, Jie Rang, Liqiu Xia   +9 more
wiley   +1 more source

Hepatotoxicity of Nonesterified Fatty Acids to Dairy Cows: Pathophysiological Mechanisms and Prospective Solutions

Animal Research and One Health, EarlyView.
Unregulated inflammation increases non‐esterified fatty acids (NEFAs), and triggers multi‐pathway hepatocyte damage including oxidative stress, mitochondrial dysfunction, and metabolic disorders in dairy cows. ABSTRACT Circulating concentrations of nonesterified fatty acids (NEFAs) are elevated due to lipid mobilization from adipose tissue in ...
Siqing Mao, Yuan Tian, Dan Li, Lei Tian, Hang Yu, Ziling Liu, Xin Gao, Yanqiong Wen, Fachun Wan, Zuo Wang, Weijun Shen, Xinwei Li, Lei Liu   +12 more
wiley   +1 more source

Synthesis of a Carbon‐Linked Acyldepsipeptide Derivative

European Journal of Organic Chemistry, EarlyView.
The synthesis of a methylene‐linked acyldepsipeptide (ADEP) is reported. Success relied upon an improve method to generate a versatile vinyl glycine methyl ester synthon and a rationally optimized Grubb’s metathesis. NMR and in silico studies revealed an unusual trans–trans proline orientation for this new unnatural ADEP analog, advancing the ...
Katelyn G. Stevens-Davis, Xiaozheng Dou, Bo Hu, Yangxiong Li, Jiayuan Miao, Yu-Shan Lin, Adam S. Duerfeldt   +6 more
wiley   +1 more source

Characterization of TR‐107, a novel chemical activator of the human mitochondrial protease ClpP

Pharmacology Research & Perspectives, 2022
We recently described the identification of a new class of small‐molecule activators of the mitochondrial protease ClpP. These compounds synthesized by Madera Therapeutics showed increased potency of cancer growth inhibition over the related compound ...
Emily M. J. Fennell, Lucas J. Aponte‐Collazo, Joshua D. Wynn, Kristina Drizyte‐Miller, Elisa Leung, Yoshimi Endo Greer, Paul R. Graves, Andrew A. Iwanowicz, Hani Ashamalla, Ekhson Holmuhamedov, Henk Lang, Donald S. Karanewsky, Channing J. Der, Walid A. Houry, Stanley Lipkowitz, Edwin J. Iwanowicz, Lee M. Graves   +16 more
doaj   +1 more source

Targeting Mitochondrial DNA Transcription by POLRMT Inhibition or Depletion as a Potential Strategy for Cancer Treatment

Biomedicines, 2023
Transcription of the mitochondrial genome is essential for the maintenance of oxidative phosphorylation (OXPHOS) and other functions directly related to this unique genome.
Sabrina C. D. Daglish, Emily M. J. Fennell, Lee M. Graves   +2 more
doaj   +1 more source

Achieving superior therapeutic effects on methicillin‐resistant Staphylococcus aureus infection via simple sulfonation process for preparing zwitterionic photosensitizer

Interdisciplinary Medicine, EarlyView.
The sulfonated zwitterionic photosensitizer (PYSO3) is synthesized by reacting maleic anhydride derivatives with 4‐aminopyridine to form PY, followed by sulfonation with 1,4‐butane sultone. It exhibits both photothermal and photodynamic properties, enabling effective eradication of MRSA and biofilms, as well as promoting wound healing in mice ...
Changxu Zhu, Yin Li, Qiyun Deng, Ruixin Guo, Yu Huang, Wenyan Guo, Xi Chen, Ben Zhong Tang, Zhiming Wang, Lidan Chen, Zhaohui Sun   +10 more
wiley   +1 more source