Results 51 to 60 of about 152,432 (174)

Structural insights into the Clp protein degradation machinery

mBio
The Clp protease system is important for maintaining proteostasis in bacteria. It consists of ClpP serine proteases and an AAA+ Clp-ATPase such as ClpC1.
Xiaolong Xu, Yanhui Wang, Wei Huang, Danyang Li, Zixin Deng, Feng Long   +5 more
doaj   +1 more source

The ClpXP protease and the ClpX unfoldase control virulence, cell division, and autolysis in Streptococcus pneumoniae

Microbiology Spectrum
In all living cells, molecular chaperones and ATP-dependent proteases are essential for protein homeostasis. The ClpXP protease is a chaperone-protease complex conserved between bacteria and mitochondria.
Viktor H. Mebus, Supradipta De, Larissa M. Busch, Manuela Gesell Salazar, Rabea Schlüter, Uwe Völker, Sven Hammerschmidt, Dorte Frees   +7 more
doaj   +1 more source

Reprogramming of the Caseinolytic Protease by ADEP Antibiotics: Molecular Mechanism, Cellular Consequences, Therapeutic Potential

Frontiers in Molecular Biosciences, 2021
Rising antibiotic resistance urgently calls for the discovery and evaluation of novel antibiotic classes and unique antibiotic targets. The caseinolytic protease Clp emerged as an unprecedented target for antibiotic therapy 15 years ago when it was ...
Heike Brötz-Oesterhelt, Heike Brötz-Oesterhelt, Andreas Vorbach   +2 more
doaj   +1 more source

Trapping and Proteomic Identification of Cellular Substrates of the ClpP Protease in Staphylococcus aureus

, 2016
In the important human pathogen Staphylococcus aureus the cytoplasmic ClpP protease is essential for mounting cellular stress responses and for virulence.
Stephan Michalik (1997962), Anders N. Varming (1997959), Lotte Jelsbak (168171), Hanne Ingmer (94052), Dirk Albrecht (256453), Michael Hecker (51971), Dorte Frees (150622), Julie H. Andersen (1997965), Stefanie Krieger (1997968), Knut Ohlsen (85866), Ulf Gerth (1997974), Jingyuan Feng (1997971)   +11 more
core   +2 more sources

ClpAP proteolysis does not require rotation of the ClpA unfoldase relative to ClpP

eLife, 2020
AAA+ proteases perform regulated protein degradation in all kingdoms of life and consist of a hexameric AAA+ unfoldase/translocase in complex with a self-compartmentalized peptidase.
Sora Kim, Kristin L Zuromski, Tristan A Bell, Robert T Sauer, Tania A Baker   +4 more
doaj   +1 more source

Chemical Inhibition of Pyocyanin Biosynthesis Attenuates Virulence of Pseudomonas aeruginosa

Angewandte Chemie International Edition, EarlyView.
Anti‐virulence strategies disarm pathogens rather than killing them directly. A β‐lactone reduces pyocyanin production without affecting growth. Chemical proteomics identified PqsC as the primary target via binding to Cys129. Crystallography revealed the binding mode, and SAR yielded a chloroacetamide inhibitor with improved enzymatic potency and anti ...
Wei Ding, Sebastian R. Borgert, Wulf Blankenfeldt, Stephan A. Sieber   +3 more
wiley   +1 more source

The Mycobacterium tuberculosis ClpP1P2 Protease Interacts Asymmetrically with Its ATPase Partners ClpX and ClpC1. [PDF]

PLoS ONE, 2015
Clp chaperone-proteases are cylindrical complexes built from ATP-dependent chaperonerings that stack onto a proteolytic ClpP double-ring core to carry out substrate protein degradation.Interaction of the ClpP particle with the chaperone is mediated by an
Julia Leodolter, Jannis Warweg, Eilika Weber-Ban   +2 more
doaj   +1 more source

The FGF/FGFR System in the Biology and Therapeutic Landscape of Pediatric CNS Tumors

Medicinal Research Reviews, EarlyView.
ABSTRACT Central nervous system (CNS) tumors are the most common solid malignancies in children, comprising a highly heterogeneous group of neoplasms defined by distinct molecular alterations and clinical behaviors. Advances in molecular genetics have underscored the relevance of specific signaling pathways in driving pediatric tumorigenesis, among ...
Serena Filiberti, Camilla Tavani, Elena Somenza, Nicolas Tassone, Antonio d'Amati, Marta Turati, Roberto Ronca   +6 more
wiley   +1 more source

Targeting the Mitochondrial Protease ClpP for Anticancer Therapy

Journal of Medicinal Chemistry
Abstract Cancer cells depend on mitochondrial reprogramming for growth, but this raises reactive oxygen species (ROS), increasing reliance on protein quality control (PQC) repair mechanisms. The mitochondrial proteome is maintained through a robust PQC composed of chaperones and proteases, including the mitochondrial matrix protease ...
Zhongli Xu, Dmitry Pokushalov, Md Kabir, Youngeun Lee, Mrittika Chattopadhyay, Edmund C. Jenkins, Cessarina Choo, H. Ümit Kaniskan, Doris Germain, Jian Jin   +9 more
openaire   +2 more sources

Placental mitochondrial metabolic adaptation maintains cellular energy balance in pregnancy complicated by gestational hypoxia

The Journal of Physiology, EarlyView.
Abstract figure legend Placental mitochondrial adaptation to gestational hypoxia. Hypoxic pregnancy in sheep increases placental insulin like growth factor 2 (IGF2) signalling (1), which is associated with a shift in capacity away from β‐oxidation (2) and complex I‐mediated respiration (3), while maintaining total oxidative phosphorylation capacity (4).
Wen Tong, Beth J. Allison, Kirsty L. Brain, Olga V. Patey, Youguo Niu, Kimberley J. Botting, Sage G. Ford, Tess A. Garrud, Peter F. B. Wooding, Qiang Lyu, Lin Zhang, Jin Ma, Alice P. Sowton, Katie A. O'Brien, Tereza Cindrova‐Davies, Hong Wa Yung, Graham J. Burton, Andrew J. Murray, Dino A. Giussani   +18 more
wiley   +1 more source