Efficient CRISPR-Cas9 Gene Disruption System in Edible-Medicinal Mushroom Cordyceps militaris
Frontiers in Microbiology, 2018 Cordyceps militaris is a well-known edible medicinal mushroom in East Asia that contains abundant and diverse bioactive compounds. Since traditional genome editing systems in C.
Bai-Xiong Chen +13 more
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Poultry Science, 2020 Avian coccidiosis causes significant economic losses on the global poultry breeding industry. Exploration of new-concept vaccines against coccidiosis has gradually become a research hotspot.
Wenjing Chen +4 more
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Template Activity of Modified Terminator Codons
Journal of Biological Chemistry, 1970 Abstract Modified trinucleotides, 3MeU-A-G+, 3MeU-A-A, 5MeU-A-G, 5MeU-A-A, BrU-A-G, hU-A-G, and U-A-I (see J. Biol. Chem., 241, 527 (1966)), were synthesized by a combination of organic and enzymatic methods. The activity of each trinucleotide as a template for peptide chain termination was determined.
J, Smrt +3 more
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Genome-wide transcription start site profiling in biofilm-grown Burkholderia cenocepacia J2315 [PDF]
, 2015 Background: Burkholderia cenocepacia is a soil-dwelling Gram-negative Betaproteobacterium with an important role as opportunistic pathogen in humans. Infections with B.
Coenye, Tom +6 more
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Harnessing the potential of chloroplast-derived expression elements for enhanced production of cellulases in Escherichia coli [PDF]
PeerJThermophilic cellulases can play a crucial part in the efficient breakdown of cellulose—a major component of lignocellulosic plant biomass, however, their commercial production needs simple and robust biomanufacturing biosystems.
Ayesha Siddiqui +10 more
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Identification of small RNAs abundant in Burkholderia cenocepacia biofilms reveal putative regulators with a potential role in carbon and iron metabolism [PDF]
, 2017 Small RNAs play a regulatory role in many central metabolic processes of bacteria, as well as in developmental processes such as biofilm formation. Small RNAs of Burkholderia cenocepacia, an opportunistic pathogenic beta-proteobacterium, are to date not ...
Coenye, Tom +2 more
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Frontiers in Genetics, 2023 Mesona chinensis Benth (MCB) (or Platostoma palustre or Platostoma chinense) is an important edible and medicinal plant in China. However, the mitochondrial genome (mitogenome, or mtDNA) of MCB has not been characterized or reported yet.
Danfeng Tang +10 more
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Enzymatic and antisense effects of a specific anti-Ki-ras ribozyme in vitro and in cell culture [PDF]
, 1999 Due to their mode of action, ribozymes show antisense effects in addition to their specific cleavage activity. In the present study we investigated whether a hammerhead ribozyme is capable of cleaving mutated Ki-ras mRNA in a pancreatic carcinoma cell ...
Giannini, Claudio Detlef +3 more
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Premature termination codons in modern human genomes [PDF]
Scientific Reports, 2016 AbstractThe considerable range of genetic variation in human populations may partly reflect distinctive processes of adaptation to variable environmental conditions. However, the adaptive genomic signatures remain to be completely elucidated. This research explores candidate loci under selection at the population level by characterizing recently arisen
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Chance and necessity in chromosomal gene distributions [PDF]
, 2008 By analyzing the spacing of genes on chromosomes, we find that transcriptional and RNA-processing regulatory sequences outside coding regions leave footprints on the distribution of intergenic distances.
Hermsen, Rutger +2 more
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