Results 11 to 20 of about 69,133 (285)

CRISPR-UnLOCK: Multipurpose Cas9-Based Strategies for Conversion of Yeast Libraries and Strains [PDF]

Frontiers in Microbiology, 2017
Saccharomyces cerevisiae continues to serve as a powerful model system for both basic biological research and industrial application. The development of genome-wide collections of individually manipulated strains (libraries) has allowed for high ...
Emily Roggenkamp, Rachael M. Giersch, Emily Wedeman, Muriel Eaton, Emily Turnquist, Madison N. Schrock, Linah Alkotami, Thitikan Jirakittisonthon, Samantha E. Schluter-Pascua, Gareth H. Bayne, Cory Wasko, Megan Halloran, Gregory C. Finnigan   +12 more
doaj   +4 more sources

The Conserved Dcw Gene Cluster of R. sphaeroides Is Preceded by an Uncommonly Extended 5’ Leader Featuring the sRNA UpsM [PDF]

, 2016
Cell division and cell wall synthesis mechanisms are similarly conserved among bacteria. Consequently some bacterial species have comparable sets of genes organized in the dcw (division and cell wall) gene cluster.
Klug, Gabriele, Lechner, Marcus, Remes, Bernhard, Thoelken, Clemens, Volk, Marcel, Weber, Lennart   +5 more
core   +12 more sources

Cytosolic re-localization and optimization of valine synthesis and catabolism enables increased isobutanol production with the yeast Saccharomyces cerevisiae [PDF]

, 2012
Background: The branched chain alcohol isobutanol exhibits superior physicochemical properties as an alternative biofuel. The yeast Saccharomyces cerevisiae naturally produces low amounts of isobutanol as a by-product during fermentations, resulting from
Bode, Helge Björn, Boles, Eckhard, Brat, Dawid, Lorenzen, Wolfram, Weber, Christian   +4 more
core   +1 more source

Isolation and characterization of strong gene regulatory sequences from apple, Malus x domestica [PDF]

, 2011
For the strong expression of genes in plant tissue, the availability of specific gene regulatory sequences is desired. We cloned promoter and terminator sequences of an apple (Malus x domestica) ribulose biphosphate carboxylase small subunit gene (MdRbcS)
Krens, F.A., Schaart, J.G., Tinnenbroek, I.E.M.   +2 more
core   +2 more sources

A Simple CRISPR/Cas9 System for Efficiently Targeting Genes of Aspergillus Section Flavi Species, Aspergillus nidulans, Aspergillus fumigatus, Aspergillus terreus, and Aspergillus niger

Microbiology Spectrum, 2023
For Aspergillus flavus, a pathogen of considerable economic and health concern, successful gene knockout work for more than a decade has relied nearly exclusively on using nonhomologous end-joining pathway (NHEJ)-deficient recipients via forced double ...
Perng-Kuang Chang
doaj   +1 more source

Site-specific selection reveals selective constraints and functionality of tumor somatic mtDNA mutations. [PDF]

, 2017
BACKGROUND: Previous studies have indicated that tumor mitochondrial DNA (mtDNA) mutations are primarily shaped by relaxed negative selection, which is contradictory to the critical roles of mtDNA mutations in tumorigenesis.
Chen, Cheng, Du, Xiaohong, Guo, Xu, Li, Bingshan, Li, Deyang, Li, Mingkun, Li, Xin, Xing, Jinliang, Yang, Hushan, Yin, Chun, Zhan, Lei   +10 more
core   +2 more sources

Peroxisomal Proteostasis Involves a Lon Family Protein That Functions as Protease and Chaperone [PDF]

, 2012
Proteins are subject to continuous quality control for optimal proteostasis. The knowledge of peroxisome quality control systems is still in its infancy. Here we show that peroxisomes contain a member of the Lon family of proteases (Pln).
Aksam, Antonenkov, Arnold, Bartoszewska, Bender, Bota, Buchner, Dobson, Ellis, Fraaije, Franke, Goldberg, Guinier, Hanson, Hartl, Hillenga, Islinger, Iyer, Jaenicke, Jahn, Kiel, Kiel, Kikuchi, Kozin, Lee, Lingard, Liu, Meijer, Nettleship, Nijland, Okumoto, Omi, Opaliński, Paraszkiewicz, Petoukhov, Petoukhov, Petoukhov, Powers, Quiocho, Reeder, Rep, Roessle, Roudiak, Sambrook, Sauer, Stahlberg, Steinberg, Svergun, Tyedmers, van Dijken, van Roermund, Venkatesh, Volkov, von Janowsky, Wagner, Walton, Wanders, Waterham, West   +58 more
core   +10 more sources

Improvement of l-ornithine production by attenuation of argF in engineered Corynebacterium glutamicum S9114

AMB Express, 2018
l-Ornithine, a non-essential amino acid, has enormous industrial applications in food, pharmaceutical, and chemical industries. Currently, l-ornithine production is focused on microorganism fermentation using Escherichia coli or Corynebacterium ...
Bin Zhang, Miao Yu, Ying Zhou, Bang-Ce Ye   +3 more
doaj   +1 more source

Release factors differing in specificity for terminator codons. [PDF]

Proceedings of the National Academy of Sciences, 1968
E, Scolnick, R, Tompkins, T, Caskey, M, Nirenberg   +3 more
openaire   +4 more sources

Recombinational cloning with plant gateway vectors [PDF]

, 2007
International ...
Karimi, Mansour, Depicker, Anna, Hilson, Pierre   +2 more
core   +3 more sources