Results 191 to 200 of about 49,031 (262)

Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐CLOVER establishes enhanced CHO platforms for cell line development

Biotechnology Progress, EarlyView.
This study established the first creation of a commercially available double GS knockout (DKO) from a single KO in CHO‐K1 cells, called CleanCut GS CHO using Cas‐CLOVER. When combined with the Harbor‐IN transposase system, CleanCut GS CHO cells demonstrated a highly antibody productivity.
Cintia Gomez Limia, Valeriya Steffey, Hsueh Che Cheng, Daniel Machado, Thomas Hart, Mark Cameron McHargue, Corey Brizzee, Jack Crawford   +7 more
wiley   +1 more source

Complete Mitochondrial Genome Sequencing of <i>Brachypelma albiceps</i> and Comparative Codon Usage Bias Analysis Across Seven Mygalomorphae Species. [PDF]

Biology (Basel)
Zhan Q, Tang Y, Zhao Y, Hou S, Huang Y, Zhao X, Chen Y, Xue X.   +7 more
europepmc   +1 more source

One‐Step and Universal Strategy for the Synthesis of Hypermodified Uracil Phosphoramidites acp3U and cmnm5U

Chemistry – A European Journal, EarlyView.
One‐step, highly efficient, and scalable synthesis of acp3U phosphoramidite is described, as well as a new alternative synthesis of cmnm5U phosphoramidite from a highly versatile intermediate. Latter could be scaled‐up, split, and aliquots turned simultaneously into cmnm5U, nm5U, and mnm5U.
Ewa Mejdr, Lena Heinickel, Thomas Carell, Ivana Mejdr   +3 more
wiley   +1 more source

Comparative analysis of codon usage bias and phylogenetic relationships in chloroplast genomes across 49 Dendrobium species. [PDF]

BMC Plant Biol
Wu C, Liu R, Li C, Shang M, Zhang Y, Bai K, Wang Y, Zhou Y, Lin Y, Duan B.   +9 more
europepmc   +1 more source

Two‐Step Synthesis of a Chiral Fluorinated Alcohol With Silica‐Supported Enzyme RrADH in Batch and Continuous Flow Mode

Chemistry – A European Journal, EarlyView.
A two‐step process was transferred from batch to flow mode for the synthesis of (R)‐2‐fluoro‐1‐phenylethanol as a benchmark, consisting of a decarboxylative fluorination in aqueous media, followed by an enantioselective biocatalytic reduction of the prochiral intermediate with RrADH immobilized on silica supraparticles.
Egzon Cermjani, Greta Nölke, Stefano Di Fiore, Christoph Deckers, Doris Hanselmann, Bettina Herbig, Susanne Wintzheimer, Thomas H. Rehm   +7 more
wiley   +1 more source

A codon usage-based approach for the stratification of Influenza A across recent spillovers. [PDF]

Comput Struct Biotechnol J
Alfonsi T, Chiara M, Bernasconi A.
europepmc   +1 more source

Mini‐Hairpin DNA: Extraordinarily Stable Structure and Its Applications

Chemistry – A European Journal, EarlyView.
Mini‐hairpin DNA fragments containing GCGAAGC and GCGAAAGC form exceptionally stable hairpin‐like structures, with only two G−C pairs and one sheared G−A pair, remaining undenatured in 7 M urea and resisting nuclease digestion. These mini‐hairpin sequences frequently appear in replication and transcription origins, influence DNA sequencing fidelity ...
Michiko Kimoto, Ichiro Hirao
wiley   +1 more source

Codon Usage Bias in Human RNA Viruses and Its Impact on Viral Translation, Fitness, and Evolution. [PDF]

Viruses
Ventoso I.
europepmc   +1 more source

Synthetic Strategies for Activity‐Based Probes to Decode Ubiquitin‐Like Modifiers

Chemistry – A European Journal, EarlyView.
ABSTRACT Ubiquitin‐like proteins (Ubls) such as SUMO, NEDD8, ISG15, URM1, UFM1, FAT10, ATG8/ATG12, and FUBI are essential regulators of cellular homeostasis, controlling processes from protein stability and trafficking to immune signaling and autophagy.
Saibal Chanda, Alan Pham, SreeNidhi Karnati, Natalia Soto Rodriguez, Alyssa Toner, Wenshe Ray Liu   +5 more
wiley   +1 more source

Comprehensive analysis of the codon usage patterns in the polyprotein coding sequences of the honeybee viruses. [PDF]

Front Vet Sci
Aktürk Dizman Y.
europepmc   +1 more source