Optimizing Cell-Free Protein Synthesis for Increased Yield and Activity of Colicins
Methods and Protocols, 2019 Colicins are antimicrobial proteins produced by Escherichia coli that hold great promise as viable complements or alternatives to antibiotics. Cell-free protein synthesis (CFPS) is a useful production platform for toxic proteins because it eliminates the
Xing Jin +2 more
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Pairing Colicins B and E5 with Bdellovibrio bacteriovorus To Eradicate Carbapenem- and Colistin-Resistant Strains of Escherichia coli [PDF]
Microbiology Spectrum, 2023 While diverse antibacterials are available in nature, each possesses their own strengths and limitations. One such antibacterial is colicins, proteinaceous toxins that are produced by strains of E. coli to subvert the growth or viability of other E. coli
Sumudu Upatissa +2 more
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In vivo processing of DNase colicins E2 and E7 is required for their import into the cytoplasm of target cells. [PDF]
PLoS ONE, 2014 DNase colicins E2 and E7, both of which appropriate the BtuB/Tol translocation machinery to cross the outer membrane, undergo a processing step as they enter the cytoplasm. This endoproteolytic cleavage is essential for their killing action.
Liliana Mora, Miklos de Zamaroczy
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Molecular and Genetic Characterization of Colicinogenic Escherichia coli Strains Active against Shiga Toxin-Producing Escherichia coli O157:H7 [PDF]
Foods, 2023 The objective of this work was to molecularly and genotypically characterize and test the inhibitory activity of six colicinogenic Escherichia coli strains (ColEc) and their partially purified colicins against STEC O157:H7 isolated from clinical human ...
Mauro D. García +5 more
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Interaction of Colicins with Bacterial Cells I. Studies with Radioactive Colicins [PDF]
Journal of Bacteriology, 1966 Maeda, Akio (Department of Genetics, University of Wisconsin, Madison), and Masayasu Nomura . Interaction of colicins with bacterial cells. I. Studies with radioactive colicins. J. Bacteriol. 91: 685–694.
Akio Maeda, M Nomura
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Colicins produced by Escherichia coli, a review [PDF]
Romanian Journal of Infectious Diseases, 2023 Bacteria produce proteins called bacteriocins and can inhibit or even kill closely related species. Among the bacteriocins, Colicins are by far the most well-characterized group.
Amal F. Ghanim +2 more
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Escherichia coli bacteriocins: antimicrobial efficacy and prevalence among isolates from patients with bacteraemia. [PDF]
PLoS ONE, 2011 Bacteriocins are antimicrobial peptides generally active against bacteria closely related to the producer. Escherichia coli produces two types of bacteriocins, colicins and microcins.
Maruška Budič +3 more
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The Biology of Colicin M and Its Orthologs
Antibiotics, 2021 The misuse of antibiotics during the last decades led to the emergence of multidrug resistant pathogenic bacteria. This phenomenon constitutes a major public health issue.
Dimitri Chérier +4 more
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Microbiology and Molecular Biology Reviews, 2007 SUMMARYColicins are proteins produced by and toxic for some strains ofEscherichia coli. They are produced by strains ofE. colicarrying a colicinogenic plasmid that bears the genetic determinants for colicin synthesis, immunity, and release. Insights gained into each fundamental aspect of their biology are presented: their synthesis, which is under SOS ...
Cascales, E. +8 more
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Scientific Reports, 2021 The interactions of the antibiotic proteins colicins/pyocins with immunity proteins is a seminal model system for studying protein–protein interactions and specificity.
Avital Shushan, Mickey Kosloff
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