Results 21 to 30 of about 288,324 (217)

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Undenatured type II collagen for knee osteoarthritis

Annals of Medicine
Introduction: Knee Osteoarthritis (OA) leads to significant pain and reduced function and affects patients’ overall quality of life (QoL). Conservative modalities are the first line of management, resorting to surgery only if they fail.
Ashim Gupta, Nicola Maffulli
doaj   +1 more source

AUTOIMMUNE ASPECTS OF COLLAGEN HOMEOSTASIS DISORDER IN UNDIFFERENTIATED CONNECTIVE TISSUE DYSPLASIA

Медицинская иммунология, 2014
. The levels of autoantibodies to collagen type I, II, III, IV and V were studied in blood plasma of 130 patients (18-32 years old) with undifferentiated connective tissue dysplasia, using immunoenzyme analysis.
A. V. Yagoda, N. N. Gladkikh
doaj   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Fourier transform infrared imaging and infrared fiber optic probe spectroscopy identify collagen type in connective tissues.

PLoS ONE, 2013
Hyaline cartilage and mechanically inferior fibrocartilage consisting of mixed collagen types are frequently found together in repairing articular cartilage.
Arash Hanifi, Helen McCarthy, Sally Roberts, Nancy Pleshko   +3 more
doaj   +1 more source

Long non-coding RNA PVT1, a molecular sponge of miR-26b, is involved in the progression of hyperglycemia-induced collagen degradation in human chondrocytes by targeting CTGF/TGF- signal ways

Innate Immunity, 2020
The current study was conducted to investigate the role of long non-coding RNA PVT1 in hyperglycemia-triggered human osteoarthritis (OA) chondrocytes. Cartilage from knee OA patients with and without diabetes, as well as normal cartilage, was obtained ...
Luo-Bin Ding, Yao Li, Guang-Yuan Liu, Tai-Hang Li, Feng Li, Jian Guan, Hua-Jun Wang   +6 more
doaj   +1 more source

The microbiome in human skin aging

FEBS Letters, EarlyView.
Age‐related skin changes encompass the well‐known visible phenotypic alterations, together with microbiome dysbiosis and a series of molecular aging hallmarks. These hallmarks characterize not only a fully stablished aged phenotype but also the skin aging process itself.
Manuel Huerta Arana, Cornelia Wiegand, Omid Omrani, Mario Fabri   +3 more
wiley   +1 more source

Efficacy and tolerability of native (undenatured) type II collagen supplementation for joint health in healthy volunteers: a randomized double-blind placebo-controlled study

Nutrition Journal
Background In non-osteoarthritic individuals, the appearance of joint discomfort with physical activity is a potential indicator of initial joint degeneration. The present study aimed to evaluate the efficacy and tolerability of native (undenatured) type-
Ingrid Möller, Kirsten Martínez, Andrea Terradillos-Guillén, Ester Costa-Larrión, Daniel Martínez-Puig, Javier Velasco-Álvarez   +5 more
doaj   +1 more source

ABCD_AU543 recognizes collagen type II in cell-produced extracellular matrix by immunofluorescence

Antibody Reports
Animal-origin-free (AOF) antibodies are key to advancing ethical and reproducible in vitro research. In this study, we evaluated the recombinant AOF antibody ABCD_AU543 for the detection of collagen type II by immunofluorescence in cell-produced ...
Kristin Schüler, Hunter Levis, Robert Bowles, João Carlos Silva, Frederico Ferreira   +4 more
doaj   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source