Results 1 to 10 of about 1,557 (180)

Patient Derived Colonoids as Drug Testing Platforms–Critical Importance of Oxygen Concentration [PDF]

Frontiers in Pharmacology, 2021
Treatment of inflammatory bowel disease (IBD) is challenging, with a series of available drugs each helping only a fraction of patients. Patients may face time-consuming drug trials while the disease is active, thus there is an unmet need for biomarkers ...
Helene Kolstad Skovdahl, Helene Kolstad Skovdahl, Shreya Gopalakrishnan, Tarjei Dahl Svendsen, Atle van Beelen Granlund, Atle van Beelen Granlund, Ingunn Bakke, Ingunn Bakke, Zekarias G. Ginbot, Silje Thorsvik, Silje Thorsvik, Silje Thorsvik, Arnar Flatberg, Arnar Flatberg, Bjørnar Sporsheim, Bjørnar Sporsheim, Jenny Ostrop, Jenny Ostrop, Tom Eirik Mollnes, Tom Eirik Mollnes, Tom Eirik Mollnes, Tom Eirik Mollnes, Arne Kristian Sandvik, Arne Kristian Sandvik, Arne Kristian Sandvik, Torunn Bruland, Torunn Bruland   +26 more
doaj   +10 more sources

Transcriptional responses of mouse proximal colon and colonoids during early whipworm infection [PDF]

mBio
Trichuriasis, caused by the parasitic nematode Trichuris trichiura, affects 429–508 million people worldwide. Although the early phase of whipworm infection is crucial for establishing infection, its underlying molecular mechanisms remain unclear.
Hyeim Jung, Joseph F. Urban, Bruce A. Rosa, Makedonka Mitreva   +3 more
doaj   +4 more sources

Physiological hypoxia improves growth and functional differentiation of human intestinal epithelial organoids [PDF]

Frontiers in Immunology, 2023
BackgroundThe epithelium in the colonic mucosa is implicated in the pathophysiology of various diseases, including inflammatory bowel diseases and colorectal cancer.
Gunnar Andreas Walaas, Shreya Gopalakrishnan, Ingunn Bakke, Ingunn Bakke, Helene Kolstad Skovdahl, Helene Kolstad Skovdahl, Arnar Flatberg, Arnar Flatberg, Ann Elisabet Østvik, Ann Elisabet Østvik, Arne Kristian Sandvik, Arne Kristian Sandvik, Arne Kristian Sandvik, Torunn Bruland, Torunn Bruland   +14 more
doaj   +3 more sources

Comprehensive protocols for culturing and molecular biological analysis of IBD patient-derived colon epithelial organoids [PDF]

Frontiers in Immunology, 2023
There are many unanswered questions regarding responses to proinflammatory signals in intestinal epithelial cells (IECs). For example, chemokines secreted by IECs upon external stimuli play multifunctional roles in both homeostasis and during ...
Shreya Gopalakrishnan, Ingunn Bakke, Ingunn Bakke, Marianne Doré Hansen, Marianne Doré Hansen, Helene Kolstad Skovdahl, Helene Kolstad Skovdahl, Atle van Beelen Granlund, Atle van Beelen Granlund, Atle van Beelen Granlund, Atle van Beelen Granlund, Arne K. Sandvik, Arne K. Sandvik, Arne K. Sandvik, Torunn Bruland, Torunn Bruland, Torunn Bruland   +16 more
doaj   +3 more sources

Investigation of Colonic Regeneration via Precise Damage Application Using Femtosecond Laser-Based Nanosurgery [PDF]

Cells, 2022
Organoids represent the cellular composition of natural tissue. So called colonoids, organoids derived from colon tissue, are a good model for understanding regeneration.
Sören Donath, Leon Angerstein, Lara Gentemann, Dominik Müller, Anna E. Seidler, Christian Jesinghaus, André Bleich, Alexander Heisterkamp, Manuela Buettner, Stefan Kalies   +9 more
doaj   +4 more sources

Patient-derived colon epithelial organoids reveal lipid-related metabolic dysfunction in pediatric ulcerative colitis [PDF]

Nature Communications
Ulcerative colitis (UC) is associated with epithelial metabolic derangements which exacerbate gut inflammation. Here, we develop colon organoid (colonoid) lines from pediatric patients with endoscopically active UC, inactive UC, and those without ...
Babajide A. Ojo, Ying Zhu, Lyong Heo, Sejal R. Fox, Yanli Qiao, Amanda Waddell, Maria E. Moreno-Fernandez, Marielle Gibson, Tracy Tran, Ashley L. Dunn, Essam I. A. Elknawy, Neetu Saini, Javier A. López-Rivera, Senad Divanovic, Yuqin Dai, Vinicio A. de Jesus Perez, Michael J. Rosen   +16 more
doaj   +2 more sources

The dietary phytochemicals carnosic acid and sulforaphane regulate inflammatory markers in ulcerative colitis patient-derived colonoids [PDF]

Frontiers in Pharmacology
IntroductionThe inflammatory bowel disease (IBD) ulcerative colitis (UC) is characterized by continuous inflammation of the colon with erosion and ulcers. Diagnosis typically occurs in patients between their late teens and mid-30s with no cure. Available
Rocío Rivera Rodríguez, Siri Sæterstad, Ann-Therese Chattergoon Ali, Linn-Karina M. Selvik, Ingunn Bakke, Ingunn Bakke, Torunn Bruland, Torunn Bruland, Jeremy J. Johnson   +8 more
doaj   +2 more sources

Tofacitinib Downregulates TNF and Poly(I:C)-Dependent MHC-II Expression in the Colonic Epithelium [PDF]

Frontiers in Immunology, 2022
Major Histocompatibility Complex (MHC)-I and -II genes are upregulated in intestinal epithelial cells (IECs) during active inflammatory bowel diseases (IBD), but little is known about how IBD-relevant pro-inflammatory signals and IBD drugs can regulate ...
Shreya Gopalakrishnan, Shreya Gopalakrishnan, Marianne Doré Hansen, Marianne Doré Hansen, Helene Kolstad Skovdahl, Helene Kolstad Skovdahl, Ingrid Aass Roseth, Atle van Beelen Granlund, Atle van Beelen Granlund, Atle van Beelen Granlund, Ann Elisabet Østvik, Ann Elisabet Østvik, Ingunn Bakke, Ingunn Bakke, Arne Kristian Sandvik, Arne Kristian Sandvik, Arne Kristian Sandvik, Torunn Bruland, Torunn Bruland   +18 more
doaj   +3 more sources

Pathogenesis of adherent-invasive Escherichia coli LF82 in human colonic epithelium is characterized by adhesive biofilms, mucus penetration, and contact-dependent cytotoxicity [PDF]

Gut Microbes
Adherent-invasive Escherichia coli (AIEC) associated with Crohn's disease (CD) are traditionally defined by the adherence and invasion of epithelial cells and survival in macrophages.
Bethan Fay Evans, Tshering Dorji, Damira Bigaliyeva, Simon Chan, Stephanie Schüller   +4 more
doaj   +2 more sources

Determinants of divergent Salmonella and Shigella epithelial colonization strategies resolved in human enteroids and colonoids [PDF]

mBio
Despite their relatedness, Salmonella and Shigella enteropathogens differ in infectious dose, pathogenesis, and disease kinetics. The prototype strains Salmonella enterica serovar Typhimurium (Salmonella) and Shigella flexneri (Shigella) use Type-3 ...
Petra Geiser, Maria Letizia Di Martino, Ana C. C. Lopes, Alexandra Bergholtz, Magnus Sundbom, Martin Skogar, Wilhelm Graf, Kajsa Björner, Johan Vessby, Dominic-Luc Webb, Per M. Hellström, Jens Eriksson, Mikael E. Sellin   +12 more
doaj   +2 more sources