Results 91 to 100 of about 1,557 (180)

Interactions between Enterohemorrhagic Escherichia coli (EHEC) and Gut Commensals at the Interface of Human Colonoids

mBio, 2022
Enterohemorrhagic E. coli (EHEC) is a natural human pathogen that poorly colonizes mice. Hence, the use of murine models to understand features of EHEC infection is a challenge.
Fernando H. Martins, Anubama Rajan, Hannah E. Carter, Hamid R. Baniasadi, Anthony W. Maresso, Vanessa Sperandio   +5 more
openaire   +3 more sources

Engineered Tissue Models to Decode Host–Microbiota Interactions

Advanced Science, Volume 12, Issue 23, June 20, 2025.
Host–Microbiota interactions in the human body. Created in BioRender. Ghezzi, C. (2025) https://BioRender.com/ihivskg. Abstract A mutualistic co‐evolution exists between the host and its associated microbiota in the human body. Bacteria establish ecological niches in various tissues of the body, locally influencing their physiology and functions, but ...
Miryam Adelfio, Grace E. Callen, Xuesong He, Bruce J. Paster, Hatice Hasturk, Chiara E. Ghezzi   +5 more
wiley   +1 more source

Organoids as a Model for Intestinal Ion Transport Physiology [PDF]

, 2020
The advent of intestinal organoid culture in 2009 was a fortuitous development in the search for a valid marker of intestinal stem cells, and provided proof of murine intestinal stem cell regenerative potential.
A Bein, A Elgavish, A Ermund, A Kapus, A Kockerling, A Ootani, A Reynolds, AD Gracz, AM Gallagher, AM Strubberg, B Lee, B van der Hee, BD Ramsey, BLN Than, BM Fihn, C Hofmann, C Vitzthum, CA Bertrand, CA Flores, CE Wainwright, CH Yun, CP Stewart, D-A Hallbäck, DD Zomer-van Ommen, EH Fernando, EV O’Loughlin, FJ Accurso, FS Collins, G Schwank, G Vega, G Wilke, GA Kim, GS Evans, H Barriere, H Cheng, H Miyoshi, H Miyoshi, H Zhao, HGM Heijerman, HJ Kim, HJ Kim, HR De Jonge, I Heo, J Foulke-Abel, J Foulke-Abel, J Linley, J Liu, J Liu, J Yin, JE Matos, JE Simpson, JF Dekkers, JF Dekkers, JG In, JH Chen, JH Poulsen, JK Foskett, JR Spence, JR Thiagarajah, JS Dutton, JS Schwarz, JY Co, K De Boeck, K Kunzelmann, K Kurashima, K Saxena, K Szászi, K Yu, K-A Kim, KE Barrett, KL VanDussen, L Ferrera, LR Avula, LR Gawenis, LW Plasschaert, M Donowitz, M Hirokawa, M Stelzner, M Zizak, MA Ainsworth, MA Engevik, MA Valverde, ME Robert, MJC Bijvelds, MJC Bijvelds, MK Estes, MK Fuller, MP Boyle, MR Dorwart, MW van der Helm, N Barker, N Petersen, NM Walker, O Basak, O Furukawa, O Mignen, PG Middleton, PG Noone, PM Quinton, PT Ikpa, R Benedetto, R Greger, R Greger, RA Gottlieb, RB Bajnath, RJ Lee, RJ MacLeod, RL Jakab, S Eklund, S Middendorp, S Yui, SK Singh, T Matsu-Ura, T Paul, T Sato, T Sato, T Sato, T Tamada, TV Strong, U Seidler, W Namkung, W Shin, X Liu, X Yin, Y Kim, Y Sui, Y Wang, Y Yin, Y Yin, Z Davoudi   +129 more
core   +2 more sources

A59 GLOBAL PROTEOMIC PROFILING OF HUMAN COLONOID MONOLAYERS UNDERGOING IN VITRO CHRONIC DAMAGE

Journal of the Canadian Association of Gastroenterology, 2023
Abstract Background An in vitro damage model has been established in our lab using human colonoids grown as 2D monolayers. Upon being subjected to repeated rounds of air-liquid interface (ALI) growth and injury by submergence, these colonoid monolayers lost their barrier integrity and regrowth ...
Sandilya, S, Steiner, T, Lange, P, Rees, W   +3 more
openaire   +1 more source

Leveraging Organ‐on‐Chip Models to Investigate Host–Microbiota Dynamics and Targeted Therapies for Inflammatory Bowel Disease

Advanced Healthcare Materials, Volume 14, Issue 10, April 15, 2025.
The challenges of studying inflammatory bowel disease (IBD), highlighting limitations of traditional 2D cell cultures and animal models are discussed here. It introduces human intestine organ‐on‐chip (OoC) models as a promising alternative, capable of more accurately mimicking the intestinal microenvironment.
Tim Kaden, Raquel Alonso‐Román, Johannes Stallhofer, Mark S. Gresnigt, Bernhard Hube, Alexander S. Mosig   +5 more
wiley   +1 more source

Calcium-Induced Differentiation in Normal Human Colonoid Cultures

, 2018
ABSTRACT Colonoid cultures were established from histologically-normal human colon tissue and maintained in a low-calcium (0.25 mM) medium or in medium supplemented with an amount of calcium (1.5 - 3.0 mM) that was shown in a previous study to induce differentiation in colonoids derived from large adenomas.
Attili, Durga, McClintock, Shannon D., Rizvi, Areeba H., Pandya, Shailja, Rehman, Humza, Nadeem, Daniyal M, Richter, Aliah, Thomas, Dafydd, Dame, Michael K., Turgeon, D. Kim, Varani, James, Aslam, Muhammad Nadeem   +11 more
openaire   +1 more source

Human colonic organoids for understanding early events of familial adenomatous polyposis pathogenesis

The Journal of Pathology, Volume 265, Issue 1, Page 26-40, January 2025.
Abstract Patients with familial adenomatous polyposis (FAP) harbor mutations in the APC gene and will develop adenoma and early colorectal cancer. There is no validated treatment, and animal models are not sufficient to study FAP. Our aim was to investigate the early events associated with FAP using the intestinal organoid model in a single‐center ...
Nolwenn Laborde, Alexandre Barusseaud, Muriel Quaranta, Corinne Rolland, Amélie Arrouy, Delphine Bonnet, Sylvain Kirzin, Nuria Sola‐Tapias, Dimitri Hamel, Karl Barange, Jean‐Pierre Duffas, Marie‐Pierre Gratacap, Julie Guillermet‐Guibert, Anne Breton, Nathalie Vergnolle, Laurent Alric, Audrey Ferrand, Frédérick Barreau, Claire Racaud‐Sultan, Emmanuel Mas   +19 more
wiley   +1 more source

Excess Dietary Sugar Alters Colonocyte Metabolism and Impairs the Proliferative Response to Damage [PDF]

, 2023
Background & Aims The colonic epithelium requires continuous renewal by crypt resident intestinal stem cells (ISCs) and transit-amplifying (TA) cells to maintain barrier integrity, especially after inflammatory damage.
Beyaz, Semir, Burr, Ansen H.P., Chiaranunt, Pailin, Cooper, Vaughn S., Cumberland, Rachel, Delgoffe, Greg M., Eskiocak, Onur, Hand, Timothy W., Ji, Junyi, Marshall, Christopher, Menk, Ashley V., Mentrup, Heather L., Mollen, Kevin P., Mullinax, Lauren, Overacre-Delgoffe, Abigail, Ozler, Kadir, Poholek, Amanda C., Rittenhouse, Natalie, Yueh, Brian, Zhang, Xiaoyi   +19 more
core   +1 more source

The Role of Bacterial Proteases From the Microbiome in Human Disease

Cellular Microbiology, Volume 2025, Issue 1, 2025.
Proteases degrade proteins and peptides, recycling materials and preventing unnecessary buildup within the cell. They can also be secreted and act in extracellular space. Bacterial proteases are often secreted and function as virulence factors. In the context of the microbiome, they can contribute to host–microbe interactions to facilitate colonization
Ying-Chiang J. Lee, Anirudha Chattopadhyay   +1 more
wiley   +1 more source

Outer Membrane Vesicles Derived From Fusobacterium nucleatum Trigger Periodontitis Through Host Overimmunity

Advanced Science, Volume 11, Issue 47, December 18, 2024.
Xiaoheng Liu, Jing Xie and co‐workers dissect the novel role of OMVs derived from F. nucleatum in the occurrence of periodontitis. The severity of periodontitis caused by OMVs is close to the effect of F. nucleatum itself, due to the activations of NLRP3 inflammasomes, inflammatory factors, and extracellular degradation.
Li Zhang, Demao Zhang, Chengcheng Liu, Boyu Tang, Yujia Cui, Daimo Guo, Mengmeng Duan, Ying Tu, Huiling Zheng, Xinjie Ning, Yang Liu, Haoran Chen, Minglei Huang, Zhixing Niu, Yanfang Zhao, Xiaoheng Liu, Jing Xie   +16 more
wiley   +1 more source