Results 101 to 110 of about 1,557 (180)

Initial pig developmental stage influences intestinal organoid growth but not cellular composition

Animal Models and Experimental Medicine, Volume 7, Issue 6, Page 944-954, December 2024.
We prepared organoids from jejunal and colonic specimens collected from pigs aged between 1 and 180 days to evaluate the impact of donor age on organoid growth, morphology and cellular composition. The age of the tissue donor affected organoid growth and morphology but did not influence its phenotype.
Camille Duchesne, Gwénaëlle Randuineau, Laurence Le Normand, Véronique Romé, Samia Laraqui, Alexis Pierre Arnaud, Gaëlle Boudry   +6 more
wiley   +1 more source

The expression of intestinal Cyp2c55 is regulated by the microbiota and inflammation

The FASEB Journal, Volume 38, Issue 20, 31 October 2024.
Cyp2c55 is highly expressed in the colonic epithelial cells of mice in a microbiota‐dependent manner. The underlying mechanism seems to involve a BA‐mediated PXR activation. Furthermore, the colonic expression of Cyp2c55 is also subject to regulation by the inflammatory response. While the physiological function of Cyp2c55 remains largely unidentified,
Adrian Hilman, Tetsu Sato, Bambang Dwi Wijatniko, So Fujimura, Katsushi Nakamura, Hiroto Miura, Ken Iwatsuki, Ryo Inoue, Takuya Suzuki   +8 more
wiley   +1 more source

Host–diet–microbiota interplay in intestinal nutrition and health

FEBS Letters, Volume 598, Issue 20, Page 2482-2517, October 2024.
Quantitative or qualitative alterations in intestinal resident microbe composition can lead to microbiota imbalance, namely intestinal dysbiosis, in both flies and humans. Intestinal microbiota dysbiosis, in turn, deregulates host physiology and contributes to the development, as well as the severity, of various types of intestinal and other diseases ...
Anastasia Ignatiou, Chrysoula Pitsouli
wiley   +1 more source

Defining enteric bacterial pathogenesis using organoids: Citrobacter rodentium uses EspC, an atypical mucinolytic protease, to penetrate mouse colonic mucus

Gut Microbes
Enteric bacterial pathogens pose significant threats to human health; however, the mechanisms by which they infect the mammalian gut in the face of daunting host defenses remain to be fully defined.
Yan Chen, Ashley Gilliland, Qiaochu Liang, Xiao Han, Hyungjun Yang, Jocelyn Chan, Dominique Lévesque, Kyung-Mee Moon, Parandis Daneshgar, François-Michel Boisvert, Leonard Foster, Wesley F. Zandberg, Kirk Bergstrom, Hong B. Yu, Bruce A. Vallance   +14 more
doaj   +1 more source

Cystathionine Gamma-Lyase Regulates TNF-α-Mediated Injury Response in Human Colonic Epithelial Cells and Colonoids

Antioxidants
Cystathionine gamma-lyase (CSE) and TNF-α are now recognized as key regulators of intestinal homeostasis, inflammation, and wound healing. In colonic epithelial cells, both molecules have been shown to influence a variety of biological processes, but the
Francisco Arroyo Almenas, Gábor Törő, Peter Szaniszlo, Manjit Maskey, Ketan K. Thanki, Walter A. Koltun, Gregory S. Yochum, Irina V. Pinchuk, Celia Chao, Mark R. Hellmich, Katalin Módis   +10 more
doaj   +1 more source

Sexual dimorphism in PAR2-dependent regulation of primitive colonic cells

Biology of Sex Differences, 2019
Background Sexual dimorphism in biological responses is a critical knowledge for therapeutic proposals. However, gender differences in intestinal stem cell physiology have been poorly studied.
Julie Noguerol, Pierre-Jean Roustan, Mikael N’Taye, Léo Delcombel, Corinne Rolland, Laura Guiraud, David Sagnat, Anissa Edir, Chrystelle Bonnart, Alexandre Denadai-Souza, Céline Deraison, Nathalie Vergnolle, Claire Racaud-Sultan   +12 more
doaj   +1 more source

A Preclinical Model to Assess Intestinal Barrier Integrity Using Canine Enteroids and Colonoids

Biology
While two-dimensional (2D) cell cultures, such as Caco-2 and Madin–Darby canine kidney (MDCK) cells are widely used in a variety of biological models, these two-dimensional in vitro systems present inherent limitations in replicating the complexities of in vivo biology.
Megan P. Corbett, Vojtech Gabriel, Vanessa Livania, David Díaz-Regañón, Abigail Ralston, Christopher Zdyrski, Dongjie Liu, Sarah Minkler, Hannah Wickham, Addison Lincoln, Karel Paukner, Todd Atherly, Maria M. Merodio, Dipak Kumar Sahoo, David K. Meyerholz, Karin Allenspach, Jonathan P. Mochel   +16 more
openaire   +4 more sources

Cultivating complexity: Advancements in establishing in vitro models for the mucus‐adhering gut microbiota

Microbial Biotechnology, Volume 17, Issue 10, October 2024.
This review discusses novel strategies to specifically target and evaluate the mucosal microbiota, such as culturomics applied to mucosal biopsies, intestinal organoids and artificial in vitro models incorporating mucus. Abstract A healthy mucus is essential for maintaining intestinal homeostasis and overall well‐being.
Marco Calvigioni, Diletta Mazzantini, Francesco Celandroni, Giovanni Vozzi, Emilia Ghelardi   +4 more
wiley   +1 more source

Recapitulation of the accessible interface of biopsy-derived canine intestinal organoids to study epithelial-luminal interactions.

PLoS ONE, 2020
Recent advances in canine intestinal organoids have expanded the option for building a better in vitro model to investigate translational science of intestinal physiology and pathology between humans and animals.
Yoko M Ambrosini, Yejin Park, Albert E Jergens, Woojung Shin, Soyoun Min, Todd Atherly, Dana C Borcherding, Jinah Jang, Karin Allenspach, Jonathan P Mochel, Hyun Jung Kim   +10 more
doaj   +1 more source

2322 Modulation of autophagy in intestinal health and inflammation

Journal of Clinical and Translational Science, 2018
OBJECTIVES/SPECIFIC AIMS: Modulation of autophagy has the potential to treat inflammatory bowel disease (IBD). IBD is characterized by dysregulated inflammatory pathways and a defective intestinal epithelial barrier.
Eliseo Castillo
doaj   +1 more source