Results 11 to 20 of about 1,557 (180)

Tofacitinib and budesonide treatment affect stemness and chemokine release in IBD patient-derived colonoids [PDF]

Scientific Reports
Restoration of the intestinal epithelial barrier is crucial for achieving mucosal healing, the therapeutic goal for inflammatory bowel disease (IBD). During homeostasis, epithelial renewal is maintained by crypt stem cells and progenitors that cease to ...
Arun Sridhar, Ingunn Bakke, Shreya Gopalakrishnan, Nimo Mukhtar Mohamud Osoble, Emilie Prytz Hammarqvist, Henrik P. Sahlin Pettersen, Arne Kristian Sandvik, Ann Elisabet Østvik, Marianne Doré Hansen, Torunn Bruland   +9 more
doaj   +4 more sources

In Vitro Polarization of Colonoids to Create an Intestinal Stem Cell Compartment. [PDF]

PLoS ONE, 2016
The polarity of proliferative and differentiated cellular compartments of colonic crypts is believed to be specified by gradients of key mitogens and morphogens.
Peter J Attayek, Asad A Ahmad, Yuli Wang, Ian Williamson, Christopher E Sims, Scott T Magness, Nancy L Allbritton   +6 more
doaj   +4 more sources

Patient-derived Colonoids From Disease-spared Tissue Retain Inflammatory Bowel Disease-specific Transcriptomic Signatures

Gastro Hep Advances, 2023
Background and Aims: A key histopathological feature of inflammatory bowel disease is damage to the mucosa, including breakdown of the epithelial barrier.
Tatiana A. Karakasheva, Yusen Zhou, Hongbo M. Xie, Gloria E. Soto, Tiana D. Johnson, Madison A. Stoltz, Daana M. Roach, Noor Nema, Chizoba N. Umeweni, Kaitlyn Naughton, Lauren Dolinsky, James A. Pippin, Andrew D. Wells, Struan F.A. Grant, Louis Ghanem, Natalie Terry, Amanda B. Muir, Kathryn E. Hamilton   +17 more
doaj   +3 more sources

Porcine colonoids and enteroids keep the memory of their origin during regeneration [PDF]

American Journal of Physiology-Cell Physiology, 2021
The development of alternative in vitro culture methods has increased in the last decade as three-dimensional organoids of various tissues, including those of the small and large intestines.
Barnett AM, Hendriks C, Le Borgne L, McNabb WC, Mullaney JA, Roy NC   +5 more
core   +5 more sources

Efficient RNA and RNA-protein co-detection in 3D colonoids by whole-mount staining

STAR Protocols, 2022
Summary: Here, we describe a protocol to visualize RNA oligos and proteins independently or together using a combination of fluorescence in situ hybridization (FISH) and immunofluorescence in human colonoids, expanding on previously published research ...
Roger Atanga, Amalia S. Parra, Julie G. In   +2 more
doaj   +3 more sources

Ulcerative Colitis-Derived Colonoid Culture: A Multi-Mineral-Approach to Improve Barrier Protein Expression [PDF]

Frontiers in Cell and Developmental Biology, 2020
BackgroundRecent studies demonstrated that Aquamin®, a calcium-, magnesium-rich, multi-mineral natural product, improves barrier structure and function in colonoids obtained from the tissue of healthy subjects.
Muhammad N. Aslam, Shannon D. McClintock, Durga Attili, Shailja Pandya, Humza Rehman, Daniyal M. Nadeem, Mohamed Ali H. Jawad-Makki, Areeba H. Rizvi, Maliha M. Berner, Michael K. Dame, Danielle Kim Turgeon, James Varani   +11 more
doaj   +4 more sources

Immunofluorescent Staining Assay of 3D Cell Culture of Colonoids Isolated from Mice Colon

Bio-Protocol
Here, we describe immunofluorescent (IF) staining assay of 3D cell culture colonoids isolated from mice colon as described previously. Primary cultures developed from isolated colonic stem cells are called colonoids.
Trisha Mehrotra, Xiaodi Shi, Didier Merlin   +2 more
doaj   +3 more sources

Identification and pharmacological characterization of a natural folipastatin as a CFTR inhibitor and potential anti-secretory agent in a human colonoid model [PDF]

BMC Complementary Medicine and Therapies
Background Overstimulation of cAMP-induced intestinal chloride secretion is a major cause of secretory diarrheas affecting both children and adults. This study aimed to investigate an anti-secretory effect of folipastatin, a natural depsidone derived ...
Saravut Satitsri, Rungtiwa Khumjiang, Nichakorn Worakajit, Bodee Nutho, Thanyada Rungrotmongkol, Patima Phainuphong, Taya Kitiyakara, Vatcharin Rukachaisirikul, Chatchai Muanprasat   +8 more
doaj   +2 more sources

Fecal luminal factors from patients with irritable bowel syndrome induce distinct gene expression of colonoids [PDF]

Neurogastroenterology & Motility, 2022
Background: Alteration of the host-microbiota cross talk at the intestinal barrier may participate in the pathophysiology of irritable bowel syndrome (IBS).
6hman, Lena, Iribarren, Cristina, Isaksson, Stefan, Magnusson, Maria K., Nordlander, Sofia, Savolainen, Otto, Simren, M., Sundin, Johanna, T\uf6rnblom, H.   +8 more
core   +4 more sources

SARS-CoV-2 Omicron BA.1 Variant Infection of Human Colon Epithelial Cells [PDF]

Viruses
The Omicron variant of SARS-CoV-2, characterized by multiple subvariants including BA.1, XBB.1.5, EG.5, and JN.1, became the predominant strain in early 2022.
Avan Antia, David M. Alvarado, Qiru Zeng, Luis A. Casorla-Perez, Deanna L. Davis, Naomi M. Sonnek, Matthew A. Ciorba, Siyuan Ding   +7 more
doaj   +3 more sources