Results 31 to 40 of about 1,557 (180)

The Viral Mimetic Polyinosinic:Polycytidylic Acid Alters the Growth Characteristics of Small Intestinal and Colonic Crypt Cultures. [PDF]

PLoS ONE, 2015
The intestinal epithelium is the first line of defense against enteric pathogens. We investigated the response of small intestinal and colonic crypt cultures to a panel of toll-like receptor ligands to assess the impact of microbial pattern recognition ...
Julie M Davies, Rebeca Santaolalla, Richard J von Furstenberg, Susan J Henning, Maria T Abreu   +4 more
doaj   +1 more source

Colonic epithelial adaptation to EGFR-independent growth induces chromosomal instability and is accelerated by prior injury

Neoplasia: An International Journal for Oncology Research, 2021
Although much is known about the gene mutations required to drive colorectal cancer (CRC) initiation, the tissue-specific selective microenvironments in which neoplasia arises remains less characterized.
Tiane Chen, Maged Zeineldin, Blake A. Johnson, Yi Dong, Akshay Narkar, Taibo Li, Jin Zhu, Rong Li, Tatianna C. Larman   +8 more
doaj   +1 more source

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy [PDF]

Journal of Visualized Experiments, 2015
The epithelium of the gastrointestinal tract is constantly renewed as it turns over. This process is triggered by the proliferation of intestinal stem cells (ISCs) and progeny that progressively migrate and differentiate toward the tip of the villi.
Mahe, Maxime M., Sundaram, Nambirajan, Watson, Carey L., Shroyer, Noah F., Helmrath, Michael A.   +4 more
openaire   +6 more sources

Interleukin-37 regulates innate immune signaling in human and mouse colonic organoids

Scientific Reports, 2021
Intestinal epithelial cells (IEC) reside in close proximity to the gut microbiota and are hypo-responsive to bacterial products, likely to prevent maladaptive inflammatory responses.
Joannie M. Allaire, Anita Poon, Shauna M. Crowley, Xiao Han, Zohreh Sharafian, Navjit Moore, Martin Stahl, Brian Bressler, Pascal M. Lavoie, Kevan Jacobson, Xiaoxia Li, Bruce A. Vallance   +11 more
doaj   +1 more source

Mucus layer modeling of human colonoids during infection with enteroaggragative E. coli [PDF]

Scientific Reports, 2020
AbstractEAEC is a common cause of diarrheal illness worldwide. Pathogenesis is believed to occur in the ileum and colon, where the bacteria adhere and form a robust aggregating biofilm. Among the multiple virulence factors produced by EAEC, the Pic serine protease has been implicated in bacterial colonization by virtue of its mucinolytic activity ...
Liu, Lixia, Saitz-Rojas, Waleska, Smith, Rachel, Gonyar, Laura, In, Julie G., Kovbasnjuk, Olga, Zachos, Nicholas C., Donowitz, Mark, Nataro, James P., Ruiz-Perez, Fernando   +9 more
openaire   +2 more sources

Optimized Culture Conditions for Improved Growth and Functional Differentiation of Mouse and Human Colon Organoids

Frontiers in Immunology, 2021
Background & AimsDiligent side-by-side comparisons of how different methodologies affect growth efficiency and quality of intestinal colonoids have not been performed leaving a gap in our current knowledge.
Sarah S. Wilson, Martha Mayo, Terry Melim, Heather Knight, Lori Patnaude, Xiaoming Wu, Lucy Phillips, Susan Westmoreland, Robert Dunstan, Edda Fiebiger, Sonia Terrillon   +10 more
doaj   +1 more source

The phenotype of the gut region is more stably retained than developmental stage in piglet intestinal organoids

Frontiers in Cell and Developmental Biology, 2022
Intestinal organoids are innovative in vitro tools to study the digestive epithelium. The objective of this study was to generate jejunum and colon organoids from suckling and weaned piglets in order to determine the extent to which organoids retain a ...
Eloïse Mussard, Eloïse Mussard, Corinne Lencina, Lise Gallo, Céline Barilly, Maryse Poli, Katia Feve, Mikael Albin, Laurent Cauquil, Christelle Knudsen, Caroline Achard, Guillaume Devailly, Laura Soler, Sylvie Combes, Martin Beaumont   +14 more
doaj   +1 more source

IRE1β negatively regulates IRE1α signaling in response to endoplasmic reticulum stress [PDF]

, 2020
IRE1β is an ER stress sensor uniquely expressed in epithelial cells lining mucosal surfaces. Here, we show that intestinal epithelial cells expressing IRE1β have an attenuated unfolded protein response to ER stress.
Acosta-Alvear, Adrienne W. Paton, Amin-Wetzel, Bertolotti, Bertolotti, Calfon, Chang, Delphine De Sutter, Eva Cloots, Feldman, Haber, Heidi De Luca, Hetz, Hollien, Hollien, Imagawa, Iwawaki, Iwawaki, James C. Paton, Jay R. Thiagarajah, Karagöz, Lee, Li, Lisbona, Lu, Mariska S. Simpson, Markus A. Seeliger, Martino, Mi, Michael J. Grey, Miyoshi, Nicole LeDuc, Paton, Paton, Phi Luong, Prischi, Sepulveda, Shemorry, Singh, Sophie Janssens, Sundaram, Sven Eyckerman, Tam, Tirasophon, Tirasophon, Tschurtschenthaler, Tsuru, Tsuru, Walter, Wang, Wang, Wayne I. Lencer, Wiseman, Yevgeniy V. Serebrenik, Yoshida   +54 more
core   +1 more source

Chronic Inflammation in Ulcerative Colitis Causes Long-Term Changes in Goblet Cell FunctionSummary

Cellular and Molecular Gastroenterology and Hepatology, 2022
Background & Aims: One of the features of ulcerative colitis (UC) is a defect in the protective mucus layer. This has been attributed to a reduced number of goblet cells (GCs).
Varsha Singh, Kelli Johnson, Jianyi Yin, Sun Lee, Ruxian Lin, Huimin Yu, Julie In, Jennifer Foulke-Abel, Nicholas C. Zachos, Mark Donowitz, Yan Rong   +10 more
doaj   +1 more source

Cover - A System for Sorting Colonoids Retain Gene Expression of IBD

Gastro Hep Advances, 2023
doaj   +2 more sources