Theratyping of the Rare CFTR Genotype A559T in Rectal Organoids and Nasal Cells Reveals a Relevant Response to Elexacaftor (VX-445) and Tezacaftor (VX-661) Combination [PDF]
, 2023 Despite the promising results of new CFTR targeting drugs designed for the recovery of F508del- and class III variants activity, none of them have been approved for individuals with selected rare mutations, because uncharacterized CFTR variants lack ...
Amato F. +7 more
core +1 more source
Fecal Luminal Factors from Patients with Gastrointestinal Diseases Alter Gene Expression Profiles in Caco-2 Cells and Colonoids [PDF]
, 2022 Previous in vitro studies have shown that the intestinal luminal content, including metabolites, possibly regulates epithelial layer responses to harmful stimuli and promotes disease.
6hman, Lena +9 more
core +2 more sources
Frontiers in Immunology, 2023 Disruption of the intestinal epithelial barrier is a hallmark of mucosal inflammation. It increases exposure of the immune system to luminal microbes, triggering a perpetuating inflammatory response.
Susana Lechuga +5 more
doaj +1 more source
Drivers of Transcriptional Variance in Human Intestinal Epithelial Organoids [PDF]
, 2021 Human intestinal epithelial organoids (enteroids and colonoids) are tissue cultures used for understanding the physiology of the human intestinal epithelium.
Arimura, Sumimasa +28 more
core +2 more sources
Organoid and enteroid modeling of Salmonella Infection [PDF]
, 2018 Salmonella are Gram-negative rod-shaped facultative anaerobic bacteria that are comprised of over 2,000 serovars. They cause gastroenteritis (salmonellosis) with headache, abdominal pain and diarrhea clinical symptoms. Salmonellosis brings a heavy burden
Yin, Y. (Yuebang), Zhou, D. (Daoguo)
core +10 more sources
Modeling inflammation and oxidative stress in gastrointestinal disease development using novel organotypic culture systems. [PDF]
, 2013 Gastroesophageal reflux disease (GERD), Barrett's esophagus (BE), graft-versus-host disease (GVHD), and inflammatory bowel diseases such as ulcerative colitis and Crohn's disease are common human gastrointestinal diseases that share inflammation as a key
Bortner, James D +6 more
core +2 more sources
Intestinal multicellular organoids to study colorectal cancer [PDF]
, 2021 Modeling colorectal cancer (CRC) using organoids has burgeoned in the last decade, providing enhanced in vitro models to study the development and possible treatment options for this type of cancer.
Alves, Maria M. +4 more
core +2 more sources
The stress polarity signaling (SPS) pathway serves as a marker and a target in the leaky gut barrier: implications in aging and cancer. [PDF]
, 2020 The gut barrier separates trillions of microbes from the largest immune system in the body; when compromised, a "leaky" gut barrier fuels systemic inflammation, which hastens the progression of chronic diseases.
Das, Soumita +9 more
core +3 more sources
Co‐Culture System of Human Enteroids/Colonoids with Innate Immune Cells
Current Protocols in Immunology, 2020 AbstractHuman intestinal enteroids derived from adult stem cells offer a relevant ex vivo system to study biological processes of the human gut. They recreate cellular and functional features of the intestinal epithelium of the small intestine (enteroids) or colon (colonoids) albeit limited by the lack of associated cell types that help maintain tissue
Janet F, Staab +4 more
openaire +3 more sources
Intestinal Organoids in Colitis Research: Focusing on Variability and Cryopreservation [PDF]
, 2021 In recent years, stem cell-derived organoids have become a cell culture standard that is widely used for studying various scientific issues that were previously investigated through animal experiments and using common tumor cell lines.
Bauer, Verena N. +9 more
core +2 more sources