Results 81 to 90 of about 117,682 (268)

Rapid Discovery of Illuminating Peptides for Instant Detection of Opioids in Blood and Body Fluids

Molecules, 2019
The United States is currently experiencing an opioid crisis, with more than 47,000 deaths in 2017 due to opioid overdoses. Current approaches for opioid identification and quantification in body fluids include immunoassays and chromatographic methods (e.
Shabnam Jafari, Yann Thillier, Yousif H. Ajena, Diedra Shorty, Jiannan Li, Jonathan S. Huynh, Bethany Ming-Choi Pan, Tingrui Pan, Kit S. Lam, Ruiwu Liu   +9 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Solid-Phase Synthetic Route to Multiple Derivatives of a Fundamental Peptide Unit

Molecules, 2010
Amino acids are Nature’s combinatorial building blocks. When substituted on both the amino and carboxyl sides they become the basic scaffold present in all peptides and proteins.
Martin J. O’Donnell, Maciej Pawłowski, Ziniu Zhou, Paweł Zajdel, William L. Scott   +4 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Heterologous Expression and Biochemical Characterization of a Novel Lytic Polysaccharide Monooxygenase from Chitinilyticum aquatile CSC-1

Microorganisms
Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that catalyze the oxidative cleavage of recalcitrant polysaccharides. There are limited reports on LPMOs capable of concurrently catalyzing the oxidative cleavage of both cellulose ...
Xuezhi Shao, Hongliang Fang, Tao Li, Liyan Yang, Dengfeng Yang, Lixia Pan   +5 more
doaj   +1 more source

Identification of Bis-Cyclic Guanidines as Antiplasmodial Compounds from Positional Scanning Mixture-Based Libraries

Molecules, 2019
The screening of more than 30 million compounds derived from 81 small molecule libraries built on 81 distinct scaffolds identified pyrrolidine bis-cyclic guanidine library (TPI-1955) to be one of the most active and selective antiplasmodial libraries ...
David L. Perry, Bracken F. Roberts, Ginamarie Debevec, Heather A. Michaels, Debopam Chakrabarti, Adel Nefzi   +5 more
doaj   +1 more source

Combinatorial screening yields discovery of 29 metal oxide photoanodes for solar fuel generation [PDF]

, 2020
Combinatorial synthesis combined with high throughput electrochemistry enabled discovery of 29 ternary oxide photoanodes, 15 with visible light response for oxygen evolution. Y₃Fe₅O₁₂ and trigonal V₂CoO₆ emerge as particularly promising candidates due to
Gregoire, John M., Guevarra, Dan, Newhouse, Paul F., Persson, Kristin A., Richter, Matthias H., Shinde, Aniketa, Stein, Helge S., Wang, Yu, Zhou, Lan   +8 more
core   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Rational, computer-aided design of multi-target ligands : poster presentation from 6th German Conference on Chemoinformatics, GCC 2010, Goslar, Germany. 7-9 November 2010 [PDF]

, 2011
Over the past two decades the “one drug – one target – one disease” concept became the prevalent paradigm in drug discovery. The main idea of this approach is the identification of a single protein target whose inhibition leads to a successful treatment ...
Achenbach, Janosch, Proschak, Ewgenij
core  

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source