Results 101 to 110 of about 1,931,388 (325)

Interpretation of in vitro concentration‐response data for risk assessment and regulatory decision‐making: Report from the 2022 IWGT quantitative analysis expert working group meeting

Environmental and Molecular Mutagenesis, EarlyView.
Abstract Quantitative risk assessments of chemicals are routinely performed using in vivo data from rodents; however, there is growing recognition that non‐animal approaches can be human‐relevant alternatives. There is an urgent need to build confidence in non‐animal alternatives given the international support to reduce the use of animals in toxicity ...
Marc A. Beal, Guangchao Chen, Kerry L. Dearfield, Min Gi, Bhaskar Gollapudi, Robert H. Heflich, Katsuyoshi Horibata, Alexandra S. Long, David P. Lovell, Barbara L. Parsons, Stefan Pfuhler, John Wills, Andreas Zeller, George Johnson, Paul A. White   +14 more
wiley   +1 more source

Severity of effect considerations regarding the use of mutation as a toxicological endpoint for risk assessment: A report from the 8th International Workshop on Genotoxicity Testing (IWGT)

Environmental and Molecular Mutagenesis, EarlyView.
Abstract Exposure levels without appreciable human health risk may be determined by dividing a point of departure on a dose–response curve (e.g., benchmark dose) by a composite adjustment factor (AF). An “effect severity” AF (ESAF) is employed in some regulatory contexts.
Barbara L. Parsons, Marc A. Beal, Kerry L. Dearfield, George R. Douglas, Min Gi, B. Bhaskar Gollapudi, Robert H. Heflich, Katsuyoshi Horibata, Michelle Kenyon, Alexandra S. Long, David P. Lovell, Anthony M. Lynch, Meagan B. Myers, Stefan Pfuhler, Alisa Vespa, Andreas Zeller, George E. Johnson, Paul A. White   +17 more
wiley   +1 more source

Citogenotoxic response of juvenile cobia Rachycentron canadum (Linnaeus, 1766) reared in two different systems

Brazilian Journal of Oceanography
Aquaculture production is continuously growing worldwide, and marine fish farming in Brazil is still in its infancy. Intensive farming conditions may cause physiological stress to the cultured organism, which can be evaluated by citogenotoxic biomarkers.
Marina Tenório Botelho, Maria José de Arruda Campos Rocha Passos, Fabio Matsu Hasue, Daniel Lemos, Edson Rodrigues, Fanny Yasumaru, Ngan van Phan, Vicente Gomes   +7 more
doaj   +1 more source

The comet assay – how to recognise “good data”

Frontiers in Genetics, 2015
Testing of potentially genotoxic materials currently involves use of in vitro assays such as the Ames test (gene mutations), the chromosome aberration assay and in vitro micronucleus assay (predominantly using human peripheral lymphocytes to detect ...
William Barfield
doaj   +1 more source

Comet assay responses as indicators of carcinogen exposure [PDF]

, 1998
Diana Anderson, Tianwei Yu, Douglas McGregor   +2 more
openalex   +1 more source

Evaluation of the standard battery of in vitro genotoxicity tests to predict in vivo genotoxicity through mathematical modeling: A report from the 8th International Workshop on Genotoxicity Testing

Environmental and Molecular Mutagenesis, EarlyView.
Abstract In human health risk assessment of chemicals and pharmaceuticals, identification of genotoxicity hazard usually starts with a standard battery of in vitro genotoxicity tests, which is needed to cover all genotoxicity endpoints. The individual tests included in the battery are not designed to pick up all endpoints.
Mirjam Luijten, Jan van Benthem, Takeshi Morita, Raffaella Corvi, Patricia A. Escobar, Yurika Fujita, Jennifer Hemmerich, Naveed Honarvar, David Kirkland, Naoki Koyama, David P. Lovell, Miriam Mathea, Andrew Williams, Stephen Dertinger, Stefan Pfuhler, Jeroen L. A. Pennings   +15 more
wiley   +1 more source

The use of comet assay in plant toxicology: recent advances

Frontiers in Genetics, 2015
The systematic study of genotoxicity in plants induced by contaminants and other stress agents has been hindered to date by the lack of reliable and robust biomarkers.
Conceição LV Santos, Bertrand ePourrut, José Miguel P. Ferreira de Oliveira   +2 more
doaj   +1 more source

Emerging applications of the single cell gel electrophoresis (Comet) assay. I. Management of invasive transitional cell human bladder carcinoma. II. Fluorescent in situ hybridization Comets for the identification of damaged and repaired DNA sequences in individual cells [PDF]

, 1998
V. J. McKelvey‐Martin, E. T. S. Ho, S. R. McKeown, Samuel Johnston, Paul J. McCarthy, Nor Fadilah Rajab, C. Stephen Downes   +6 more
openalex   +1 more source

The impact of lymphocyte isolation on induced DNA damage in human blood samples measured by the comet assay.

Mutagenesis, 2016
The comet assay is frequently used in human biomonitoring for the detection of exposure to genotoxic agents. Peripheral blood samples are most frequently used and tested either as whole blood or after isolation of lymphocytes (i.e.
Julia Bausinger, G. Speit
semanticscholar   +1 more source

The comet assay in male reproductive toxicology

Cell Biology and Toxicology, 2007
Due to our lifestyle and the environment we live in, we are constantly confronted with genotoxic or potentially genotoxic compounds. These toxins can cause DNA damage to our cells, leading to an increase in mutations. Sometimes such mutations could give rise to cancer in somatic cells.
Adolf Baumgartner, Diana Anderson, Eduardo Cemeli   +2 more
openaire   +4 more sources