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Biochemical Society Transactions, 2009
The single-cell gel electrophoresis technique or comet assay is widely regarded as a quick and reliable method of analysing DNA damage in individual cells. It has a proven track record from the fields of biomonitoring to nutritional studies. The assay operates by subjecting cells that are fixed in agarose to high salt and detergent lysis, thus removing
Darragh G, McArt +4 more
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The single-cell gel electrophoresis technique or comet assay is widely regarded as a quick and reliable method of analysing DNA damage in individual cells. It has a proven track record from the fields of biomonitoring to nutritional studies. The assay operates by subjecting cells that are fixed in agarose to high salt and detergent lysis, thus removing
Darragh G, McArt +4 more
openaire +2 more sources
2023
The comet assay is a sensitive, inexpensive, fast, and easy-to-use method widely used in genotoxicity testing and biomonitoring studies. It can be applied using a few cells and on almost every cell type to assess a range of different DNA lesions, DNA repair activity and even DNA methylation levels, at the individual cell level. The principle is simple,
Costa, S, Pires, J, Teixeira-Gomes, A
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The comet assay is a sensitive, inexpensive, fast, and easy-to-use method widely used in genotoxicity testing and biomonitoring studies. It can be applied using a few cells and on almost every cell type to assess a range of different DNA lesions, DNA repair activity and even DNA methylation levels, at the individual cell level. The principle is simple,
Costa, S, Pires, J, Teixeira-Gomes, A
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Environmental Pollution
The paper provides the first assessment of the occurrence of hormetic dose responses using the Comet assay, a genotoxic assay. Using a priori evaluative criteria based on the Hormetic Database on peer-reviewed comet assay experimental findings, numerous examples of hormetic dose responses were obtained.
Edward J. Calabrese, Paul B. Selby
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The paper provides the first assessment of the occurrence of hormetic dose responses using the Comet assay, a genotoxic assay. Using a priori evaluative criteria based on the Hormetic Database on peer-reviewed comet assay experimental findings, numerous examples of hormetic dose responses were obtained.
Edward J. Calabrese, Paul B. Selby
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2009
Among the manifold advantages of the Comet assay is the ability to evaluate very limited amounts of test agents. In many cases compounds for a research study are not commercially available and the chemicals must be individually synthesised at great expense and with large amounts of time involved.
Elizabeth D. Wagner, Michael J. Plewa
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Among the manifold advantages of the Comet assay is the ability to evaluate very limited amounts of test agents. In many cases compounds for a research study are not commercially available and the chemicals must be individually synthesised at great expense and with large amounts of time involved.
Elizabeth D. Wagner, Michael J. Plewa
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Miniaturizing the comet assay with 3D vertical comets
Cytometry Part A, 2002AbstractBackgroundThe comet or single‐cell gel electrophoresis assay is a sensitive method for the detection of DNA damage. The main drawback of comet sampling is the low cell density necessary to prevent nucleus overlap after electrophoresis, which limits large‐scale high throughput screening. Another problem may be inconsistent comet focusing.
Philippe, Baert, Patrick, Van Oostveldt
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Comet assay and early apoptosis
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2001The comet assay is a single cell gel electrophoresis test currently used as a qualitative and quantitative genotoxicity test. However, some of the results from this comet assay and current knowledge on apoptosis lead us to suspect the presence of some false positive results.
P, Choucroun +4 more
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Comet assay in neonatal sepsis
The Indian Journal of Pediatrics, 2010To determine whether the DNA damage detected using the Comet assay helps in the diagnosis of neonatal sepsisSepsis was diagnosed, based on clinical findings, laboratory findings and positive culture results, in 24 of the 48 newborns who participated in the study.
Natália C, Carvalho +3 more
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Acellular comet assay: a tool for assessing variables influencing the alkaline comet assay
Radiation Protection Dosimetry, 2011In this study, an acellular modification to the alkaline comet assay to further evaluate key variables within the assay that may influence the outcome of genotoxicity studies is described. This acellular comet assay can detect differences of 0.2 Gy of (60)Co gamma-ray radiation between 0 and 1 Gy and differences of 1 Gy between 0 and 8 Gy; thus, this ...
Erin K, Kennedy +4 more
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2018
The comet assay is one of the techniques that has been extensively used to assess DNA damage in both the somatic cells and the spermatozoa over the past years. The results are robust and, as difference with other strategies to assess DNA damage, information about the presence of single-strand breaks (SSBs) or double-strand breaks (DSBs) can be obtained.
Elva I. Cortés-Gutiérrez +2 more
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The comet assay is one of the techniques that has been extensively used to assess DNA damage in both the somatic cells and the spermatozoa over the past years. The results are robust and, as difference with other strategies to assess DNA damage, information about the presence of single-strand breaks (SSBs) or double-strand breaks (DSBs) can be obtained.
Elva I. Cortés-Gutiérrez +2 more
openaire +1 more source