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Mutagenesis, 2023
Measurement of DNA migration in the comet assay can be done by image analysis or visual scoring. The latter accounts for 20-25% of the published comet assay results. Here we assess the intra- and inter-investigator variability in visual scoring of comets.
P. Møller +24 more
semanticscholar +1 more source
Measurement of DNA migration in the comet assay can be done by image analysis or visual scoring. The latter accounts for 20-25% of the published comet assay results. Here we assess the intra- and inter-investigator variability in visual scoring of comets.
P. Møller +24 more
semanticscholar +1 more source
Mutagenesis, 2019
The comet assay is widely used in studies on genotoxicity testing, human biomonitoring and clinical studies. The simple version of the assay detects a mixture of DNA strand breaks and alkali-labile sites; these lesions are typically described as DNA ...
P. Møller, H. Stopper, A. Collins
semanticscholar +1 more source
The comet assay is widely used in studies on genotoxicity testing, human biomonitoring and clinical studies. The simple version of the assay detects a mixture of DNA strand breaks and alkali-labile sites; these lesions are typically described as DNA ...
P. Møller, H. Stopper, A. Collins
semanticscholar +1 more source
Biochemical Society Transactions, 2009
The single-cell gel electrophoresis technique or comet assay is widely regarded as a quick and reliable method of analysing DNA damage in individual cells. It has a proven track record from the fields of biomonitoring to nutritional studies. The assay operates by subjecting cells that are fixed in agarose to high salt and detergent lysis, thus removing
Darragh G, McArt +4 more
openaire +2 more sources
The single-cell gel electrophoresis technique or comet assay is widely regarded as a quick and reliable method of analysing DNA damage in individual cells. It has a proven track record from the fields of biomonitoring to nutritional studies. The assay operates by subjecting cells that are fixed in agarose to high salt and detergent lysis, thus removing
Darragh G, McArt +4 more
openaire +2 more sources
, 2018
The comet assay is one of the techniques that has been extensively used to assess DNA damage in both the somatic cells and the spermatozoa over the past years. The results are robust and, as difference with other strategies to assess DNA damage, information about the presence of single-strand breaks (SSBs) or double-strand breaks (DSBs) can be obtained.
E. Cortés-Gutiérrez +2 more
semanticscholar +2 more sources
The comet assay is one of the techniques that has been extensively used to assess DNA damage in both the somatic cells and the spermatozoa over the past years. The results are robust and, as difference with other strategies to assess DNA damage, information about the presence of single-strand breaks (SSBs) or double-strand breaks (DSBs) can be obtained.
E. Cortés-Gutiérrez +2 more
semanticscholar +2 more sources
2023
The comet assay is a sensitive, inexpensive, fast, and easy-to-use method widely used in genotoxicity testing and biomonitoring studies. It can be applied using a few cells and on almost every cell type to assess a range of different DNA lesions, DNA repair activity and even DNA methylation levels, at the individual cell level. The principle is simple,
Costa, S, Pires, J, Teixeira-Gomes, A
openaire +1 more source
The comet assay is a sensitive, inexpensive, fast, and easy-to-use method widely used in genotoxicity testing and biomonitoring studies. It can be applied using a few cells and on almost every cell type to assess a range of different DNA lesions, DNA repair activity and even DNA methylation levels, at the individual cell level. The principle is simple,
Costa, S, Pires, J, Teixeira-Gomes, A
openaire +1 more source

