Results 121 to 130 of about 392,488 (288)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

正常ヒト肝細胞による補体D因子産生(予報)(自然科学系) [PDF]

, 2000
The biosynthesis of complement components by normal human hepatocytes was studied in vitro. In culture supernatants of normal hepatocytes [human primary hepatocytes (epithelial) cells] , many complement components including factor D were distinctly ...
北村, 肇, 北野, 悦子, Kitamura, Hajime, Kitano, Etsuko   +3 more
core  

Ecotin, a microbial inhibitor of serine proteases, blocks multiple complement dependent and independent microbicidal activities of human serum.

PLoS Pathogens, 2019
Ecotin is a serine protease inhibitor produced by hundreds of microbial species, including pathogens. Here we show, that ecotin orthologs from Escherichia coli, Yersinia pestis, Pseudomonas aeruginosa and Leishmania major are potent inhibitors of MASP-1 ...
Zoltán Attila Nagy, Dávid Szakács, Eszter Boros, Dávid Héja, Eszter Vígh, Noémi Sándor, Mihály Józsi, Gábor Oroszlán, József Dobó, Péter Gál, Gábor Pál   +10 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Inhibition of monocyte complement receptor enhancement by low molecular weight material from human lung cancers [PDF]

, 1981
We have studied the effect of dialysates from lung cancer homogenates to alter both the expression of complement (C3b) receptors per se and also to inhibit leucoattractant-induced enhancement of complement rosettes on monocytes from healthy individuals ...
Kay, A B, Abell, C A, Glass, E J
core  

Identification of potential diagnostic biomarkers for hypertension via integrated analysis of gene expression and DNA methylation

Blood Pressure
Objective Hypertension refers to the elevated blood pressure (BP) in arteries, with a BP reading of 140/90 mm Hg or higher in adults. Over 40% of >25-year-old population have suffered from hypertension.
Xiujiang Han, Jing Xue, Sheng Gao, Yongjian Li, Yuehe Duo, Feifei Gao   +5 more
doaj   +1 more source

Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation. [PDF]

Hypertens Res, 2023
Wang Z, Zhang Z, Li Y, Zhang Y, Wei M, Li H, Yang S, Zhou Y, Zhou X, Xing G.   +9 more
europepmc   +1 more source

AscF in the mycobacterial CIII–CIV supercomplex lacks metal and nucleotide binding but links malate oxidation to respiration

FEBS Letters, EarlyView.
The mycobacterial CIII‐CIV respiratory supercomplex is an obligate assembly, encompassing several subunits of unknown functions. We have characterized the intracellular subunit AscF, and show that it is unlikely to be a sensor for metals or nucleotides, but is required for growth on nonfermentable energy sources, and likely works as an adapter for ...
Eni Rile, Riccardo Diamanti, Daniel Lundin, Soni Sharma, Robin Lissner, Coenraad P. Kuil, Martin Högbom, Dirk Bald, Dan Sjöstrand   +8 more
wiley   +1 more source

Association between systemic complement levels and choroidal thickness in advanced non-neovascular age-related macular degeneration

Scientific Reports
To investigate associations between systemic complement activation and choroidal thickness (CT) in advanced non-neovascular age-related macular degeneration (nnAMD).
Talisa E de Carlo Forest, A. Itzam Marin, Zafar Gill, Ramya Gnanaraj, Jennifer L. Patnaik, Felix Poppelaars, Anne M. Lynch, Alan G. Palestine, Marc T. Mathias, Niranjan Manoharan, Ashley A. Frazer-Abel, V. Michael Holers, Naresh Mandava   +12 more
doaj   +1 more source

Complement factor D regulates collagen type I expression and fibroblast migration to enhance human tendon repair and healing outcomes. [PDF]

Front Immunol, 2023
Chen J, Wang J, Hart DA, Zhou Z, Ackermann PW, Ahmed AS.   +5 more
europepmc   +1 more source