Results 31 to 40 of about 550,405 (246)

Acquisition and annotation in high resolution in vivo digital biopsy by confocal microscopy for diagnosis in oral precancer and cancer

Frontiers in Oncology, 2023
IntroductionScanned fibre endomicroscopes are full point-scanning confocal microscopes with submicron lateral resolution with an optical slice thickness thin enough to isolate individual cell layers, allow active positioning of the optical slice in the z-
Tami Yap, Tami Yap, Ivy Tan, Ivy Tan, Rishi S. Ramani, Nirav Bhatia, Paula Demetrio de Souza Franca, Paula Demetrio de Souza Franca, Chris Angel, Caroline Moore, Thomas Reiner, Lindsay Bussau, Michael J. McCullough, Michael J. McCullough   +13 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Aggressive prostate cancer is associated with pericyte dysfunction

Molecular Oncology, EarlyView.
Tumor‐produced TGF‐β drives pericyte dysfunction in prostate cancer. This dysfunction is characterized by downregulation of some canonical pericyte markers (i.e., DES, CSPG4, and ACTA2) while maintaining the expression of others (i.e., PDGFRB, NOTCH3, and RGS5).
Anabel Martinez‐Romero, Ane Martinez‐Larrinaga, Joaquim Grego‐Bessa, Saioa Garcia‐Longarte, Hielke van Splunder, Ianire Astobiza, Amaia Ercilla, Laura Bozal‐Basterra, Isabel Mendizabal, Pilar Villacampa, Arkaitz Carracedo, Mariona Graupera   +11 more
wiley   +1 more source

Observation of Real Contact Area Using Laser Microscope with Wide Field of View

Journal of Advanced Mechanical Design, Systems, and Manufacturing, 2011
In tribology, a contact condition between mating surfaces is very important to reduce friction and raise their lifetime. Direct observation of apparent contact area is necessary for a better understanding of the contact condition.
Isami NITTA, Rintaro EBUCHI, Yukiko YASUDA, Hirotoshi TERAO   +3 more
doaj   +1 more source

Ex Vivo Murine Skin Model for B. burgdorferi Biofilm

Antibiotics, 2020
Borrelia burgdorferi, the causative agent of Lyme disease, has been recently shown to form biofilm structures in vitro and in vivo. Biofilms are tightly clustered microbes characterized as resistant aggregations that allow bacteria to withstand harsh ...
Jason P. Torres, Alireza G. Senejani, Gauri Gaur, Michael Oldakowski, Krithika Murali, Eva Sapi   +5 more
doaj   +1 more source

Spatial Correlations and Distribution of Competence Gene Expression in Biofilms of Streptococcus mutans

Frontiers in Microbiology, 2021
Streptococcus mutans is an important pathogen in the human oral biofilm. It expresses virulent behaviors that are linked to its genetic competence regulon, which is controlled by comX. Expression of comX is modulated by two diffusible signaling peptides,
Ivan P. Ishkov, Justin R. Kaspar, Stephen J. Hagen   +2 more
doaj   +1 more source

LINC01116, a hypoxia‐lncRNA marker of pathological lymphangiogenesis and poor prognosis in lung adenocarcinoma

Molecular Oncology, EarlyView.
The LINC01116 long noncoding RNA is induced by hypoxia and associated with poor prognosis and high recurrence rates in two cohorts of lung adenocarcinoma patients. Here, we demonstrate that besides its expression in cancer cells, LINC01116 is markedly expressed in lymphatic endothelial cells of the tumor stroma in which it participates in hypoxia ...
Marine Gautier‐Isola, Rafael Lopes Goncalves, Marin Truchi, Caroline Lacoux, Célia Scribe, Hugo Cadis, Laetitia Guardini, Sophie Bekisz, Marius Ilié, Paul Hofman, Georges Vassaux, Bernard Mari, Roger Rezzonico   +12 more
wiley   +1 more source

Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma

Molecular Oncology, EarlyView.
Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken, Andrea Abel Gutierrez, Imke van Rossum, Cornelia G. Spruijt, Michiel Vermeulen, Guido van Mierlo, Blanca Scheijen, Annemiek B. van Spriel   +7 more
wiley   +1 more source

Virtual Histology Staining of Skin Tissue using Ex Vivo Confocal Microscopy and Deep Learning [PDF]

Journal of Biomedical Physics and Engineering
Background: The use of Hematoxylin-and-Eosin (H&E) staining is widely accepted as the most reliable method for diagnosing pathological tissues. However, the conventional H&E staining process for tissue sections is time-consuming and requires significant ...
Mahmoud Bagheri, Alireza Ghanadan, Mobin Saboohi, Maryam Daneshpazhooh, Fatemeh Atyabi, Marjaneh Hejazi   +5 more
doaj   +1 more source

Algoritmo de detección de líneas aplicado a la mejora de los procesos de calibración del microscopio LEXT OLYMPUS.

Tecnología y Ciencia, 2021
En este trabajo se presenta un método para procesar imágenes para optimizar la calibración de microscopios ópticos Lext Olympus con plantillas. El algoritmo propuesto ya funciona en las escalas de los autocolimadores Nikon, que son similares a las ...
Guillermo Juan Bergues, Clemar Schürrer, Juan Caselles, Nancy Brambilla   +3 more
doaj   +1 more source