Results 31 to 40 of about 720,373 (345)

Digital confocal microscope

open access: yesOptics Express, 2012
We demonstrate experimentally a scanning confocal microscopy technique based on digital holography. The method relies on digital holographic recording of the scanned spot. The data collected in this way contains all the necessary information to digitally produce three-dimensional images. Several methods to treat the data are presented.
Alexandre S, Goy, Demetri, Psaltis
openaire   +2 more sources

An update: improvements in imaging perfluorocarbon-mounted plant leaves with implications for studies of plant pathology, physiology, development and cell biology.

open access: yesFrontiers in Plant Science, 2014
Plant leaves are optically complex, which makes them difficult to image by light microscopy. Careful sample preparation is therefore required to enable researchers to maximise the information gained from advances in fluorescent protein labelling, cell ...
George R Littlejohn   +9 more
doaj   +1 more source

Acquisition and annotation in high resolution in vivo digital biopsy by confocal microscopy for diagnosis in oral precancer and cancer

open access: yesFrontiers in Oncology, 2023
IntroductionScanned fibre endomicroscopes are full point-scanning confocal microscopes with submicron lateral resolution with an optical slice thickness thin enough to isolate individual cell layers, allow active positioning of the optical slice in the z-
Tami Yap   +13 more
doaj   +1 more source

A three-dimensional image processing program for accurate, rapid, and semi-automated segmentation of neuronal somata with dense neurite outgrowth

open access: yesFrontiers in Neuroanatomy, 2015
Three-dimensional (3-D) image analysis techniques provide a powerful means to rapidly and accurately assess complex morphological and functional interactions between neural cells.
James D. Ross   +9 more
doaj   +1 more source

Observation of Real Contact Area Using Laser Microscope with Wide Field of View

open access: yesJournal of Advanced Mechanical Design, Systems, and Manufacturing, 2011
In tribology, a contact condition between mating surfaces is very important to reduce friction and raise their lifetime. Direct observation of apparent contact area is necessary for a better understanding of the contact condition.
Isami NITTA   +3 more
doaj   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

open access: yesFEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue   +6 more
wiley   +1 more source

Self-Supervised Joint Learning for pCLE Image Denoising

open access: yesSensors
Probe-based confocal laser endoscopy (pCLE) has emerged as a powerful tool for disease diagnosis, yet it faces challenges such as the formation of hexagonal patterns in images due to the inherent characteristics of fiber bundles.
Kun Yang   +4 more
doaj   +1 more source

Confocal laser microscope

open access: yesIndian Journal of Dermatology, Venereology and Leprology, 2006
The confocal laser microscope is a novel and interesting noninvasive tool for imaging skin lesions and subsurface skin lesions that are not visible to the naked eye or even by dermoscopy. Skin can be imaged in vivo or freshly biopsied (in vitro) skin specimens can be visualized immediately without the processing that is required for routine ...
Rachita, Misri   +2 more
openaire   +2 more sources

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

open access: yesFEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva   +5 more
wiley   +1 more source

From 2D to 3D: Promising Advances in Imaging Lung Structure

open access: yesFrontiers in Medicine, 2020
The delicate structure of murine lungs poses many challenges for acquiring high-quality images that truly represent the living lung. Here, we describe several optimized procedures for obtaining and imaging murine lung tissue.
Timothy Klouda   +12 more
doaj   +1 more source

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