Results 51 to 60 of about 243,069 (278)

Detection of lumpy skin disease virus in cattle using real-time polymerase chain reaction and serological diagnostic assays in different governorates in Egypt in 2017 [PDF]

Veterinary World, 2019
Background and Aim: Lumpy skin disease (LSD), is a highly infectious viral disease of cattle, caused by LSD virus (LSDV) which belongs to the genus Capripoxvirus of family Poxviridae. In the summer of 2017, skin lesions suggestive of LSD were observed in
Gamil Sayed Gamil Zeedan, Ayman Hamid Mahmoud, Abeer Mostafa Abdalhamed, Khaled Abd El-Hamid Abd El-Razik, Manal Hamdy Khafagi , Hala Abdoula Ahmed Abou Zeina   +5 more
doaj   +1 more source

Detection of Bcl-2 gene in leukaemic rats using an EvaGreen real-time RT-PCT assay [PDF]

, 2011
Bcl-2 is an anti-apoptotic gene that is involved in the apoptosis process. Suppression of apoptosis by anti- apoptotic gene can contribute to the occurrence of diseases such as leukaemia.
Al-Salih, Hutheyfa A. H., Hamzah, Hazilawati, Haron, Nursyuhada, Mohamed Mustapha, Noordin, Sabri, Jasni, Shaari, Mohd Rosly, Sithambaram, Shanmugavelu   +6 more
core  

Complexities associated with expression of Epstein-Barr virus (EBV) lytic origins of DNA replication. [PDF]

, 2007
EBV has two lytic origins (oriLyt) of DNA replication lying at divergent sites on the viral genome within a duplicated sequence (DS). The latter contains potential hairpin loops, ‘hinge’ elements and the promoters for transcripts from viral genes BHLF1 ...
Griffin, BE, Xue, SA
core   +2 more sources

Nicotinamide N‐methyltransferase promotes drug resistance in lung cancer, as revealed by nascent proteomic profiling

Molecular Oncology, EarlyView.
AZD9291 has shown promise in targeted cancer therapy but is limited by resistance. In this study, we employed metabolic labeling and LC–MS/MS to profile time‐resolved nascent protein perturbations, allowing dynamic tracking of drug‐responsive proteins. We demonstrated that increased NNMT expression is associated with drug resistance, highlighting NNMT ...
Zhanwu Hou, Zhen Wang, Fei Yang, Xiao Han, Lei Li, Huadong Liu   +5 more
wiley   +1 more source

Rapid differential diagnosis between extrapulmonary tuberculosis and focal complications of brucellosis using a multiplex real-time PCR assay. [PDF]

PLoS ONE, 2009
BACKGROUND: Arduous to differ clinically, extrapulmonary tuberculosis and focal complications of brucellosis remain important causes of morbidity and mortality in many countries. We developed and applied a multiplex real-time PCR assay (M RT-PCR) for the
María Isabel Queipo-Ortuño, Juan D Colmenero, Pilar Bermudez, María José Bravo, Pilar Morata   +4 more
doaj   +1 more source

Liquid biopsy epigenetics: establishing a molecular profile based on cell‐free DNA

Molecular Oncology, EarlyView.
Cell‐free DNA (cfDNA) fragments in plasma from cancer patients carry epigenetic signatures reflecting their cells of origin. These epigenetic features include DNA methylation, nucleosome modifications, and variations in fragmentation. This review describes the biological properties of each feature and explores optimal strategies for harnessing cfDNA ...
Christoffer Trier Maansson, Anders Lade Nielsen, Boe Sandahl Sorensen   +2 more
wiley   +1 more source

A highly efficient and accurate method of detecting and subtyping Influenza A pdm H1N1 and H3N2 viruses with newly emerging mutations in the matrix gene in Eastern Taiwan

PLoS ONE, 2023
The rapid identification of Influenza A virus and its variants, which cause severe respiratory diseases, is imperative to providing timely treatment and improving patient outcomes.
Hui-Hua Yang, I-Tsong Huang, Ren-Chieh Wu, Li-Kuang Chen   +3 more
doaj   +2 more sources

Evaluation of real-time PCR targeting the lipL32 gene for diagnosis of Leptospira infection

BMC Microbiology, 2020
Background Different diagnostic methods have been used for the laboratory confirmation of leptospirosis. Molecular diagnostic techniques are not only faster and more sensitive than culture analysis, but can also detect a Leptospira infection before the ...
Daša Podgoršek, Eva Ružić-Sabljić, Mateja Logar, Andrea Pavlović, Tatjana Remec, Zvonko Baklan, Emil Pal, Tjaša Cerar   +7 more
doaj   +1 more source

Expression of circulating CD146 associated with endovascular dysfunction in adenine-induced chronic renal damage in rats using an EvaGreen real-time RT-PCR assay [PDF]

, 2011
The integrity of the endothelial layer of the blood vessel, intima, is critical since damage can lead to atherosclerosis. Assessment of its integrity is therefore very important although this can not be done through routine diagnostic analysis.
Hamzah, Hazilawati, Mohamed Mustapha, Noordin, P., Farah Dina, Shaari, Mohd Rosly, Sithambaram, Shanmugavelu   +4 more
core  

The role of miR-24 as a race related genetic factor in prostate cancer. [PDF]

, 2017
The incidence of prostate cancer (PCa) among African-Americans (AfA) is significantly higher than Caucasian-Americans (CaA) but the genetic basis for this disparity is not known.
Dahiya, Rajvir, Dasgupta, Pritha, Deng, Guoren, Hashimoto, Yutaka, Kato, Taku, Kulkarni, Priyanka, Kumar, Deepak, Majid, Shahana, Mitsui, Yozo, Saini, Sharanjot, Shahryari, Varahram, Shiina, Marisa, Sumida, Mitsuho, Tabatabai, Laura, Tanaka, Yuichiro, Yamamura, Soichiro   +15 more
core   +1 more source