Results 11 to 20 of about 44,957 (175)

Transmissible Gastroenteritis Coronavirus RNA-Dependent RNA Polymerase and Nonstructural Proteins 2, 3, and 8 Are Incorporated into Viral Particles [PDF]

Journal of Virology, 2012
ABSTRACT Coronavirus replication and transcription are processes mediated by a protein complex, with the RNA-dependent RNA polymerase (RdRp) as a main component. Proteomic analysis of highly purified transmissible gastroenteritis virus showed the RdRp to be a component of the viral particles.
Nogales, Aitor, Márquez-Jurado, Silvia, Galán, Carmen, Enjuanes Sánchez, Luis, Almazán, Fernando   +4 more
openaire   +4 more sources

Remdesivir is a direct-acting antiviral that inhibits RNA-dependent RNA polymerase from severe acute respiratory syndrome coronavirus 2 with high potency [PDF]

Journal of Biological Chemistry, 2020
Effective treatments for coronavirus disease 2019 (COVID-19) are urgently needed to control this current pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Replication of SARS-CoV-2 depends on the viral RNA-dependent RNA polymerase (RdRp), which is the likely target of the investigational nucleotide analogue remdesivir ...
Calvin J Gordon, Egor P Tchesnokov, Emma Woolner   +2 more
exaly   +3 more sources

Computational insights of phytochemical-driven disruption of RNA-dependent RNA polymerase-mediated replication of coronavirus: a strategic treatment plan against coronavirus disease 2019

New Microbes and New Infections, 2021
The current pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has raised global health concerns. RNA-dependent RNA polymerase (RdRp) is the prime component of viral replication/proliferation machinery and is considered to be a potential drug target against SARS-CoV-2.
A. Balkrishna, R. Mittal, G. Sharma, V. Arya   +3 more
exaly   +4 more sources

Correction: Template-dependent inhibition of coronavirus RNA-dependent RNA polymerase by remdesivir reveals a second mechanism of action. [PDF]

J Biol Chem, 2021
Tchesnokov EP, Gordon CJ, Woolner E, Kocincova D, Perry JK, Feng JY, Porter DP, Götte M.   +7 more
europepmc   +3 more sources

Prediction of small molecule inhibitors targeting the novel coronavirus (SARS-CoV-2) RNA-dependent RNA polymerase

, 2020
The current COVID-19 outbreak calls for a multi-disciplinary approach towards the design and development of novel anti-COVID therapeutics including vaccines and small molecule inhibitors targeting the viral proteins of causative agent, SARS-CoV-2. Using a combination of bioinformatics and computational tools, we have modelled the 3-D structure of the ...
Mohammed Ahmad, Abhisek Dwivedy, Richard Mariadasse, Satish Tiwari, Jeyaraman Jeyakanthan, Bichitra Kumar Biswal   +5 more
openaire   +3 more sources

RNA-dependent RNA polymerase activity in coronavirus- infected cells [PDF]

Journal of Virology, 1982
An enzymatic activity which incorporates [3H]UMP into acid-precipitable material in the presence of endogenous template was found in the cytoplasm of porcine cells infected with the transmissible gastroenteritis virus of swine. This activity was not found in uninfected control cells, nor was it found in purified virus.
D E, Dennis, D A, Brian
openaire   +2 more sources

Deciphering inhibitory mechanism of coronavirus replication through host miRNAs-RNA-dependent RNA polymerase (RdRp) interactome

Frontiers in Genetics, 2022
Abstract Despite what we know so far, Covid-19, caused by SARS-CoV-2 virus, remains a pandemic that still require urgent healthcare intervention. The frequent mutations of the SARS-CoV-2 virus has rendered disease control with vaccines and antiviral drugs quite difficult and challenging, with newer variants surfacing constantly.
Morenikeji, Olanrewaju B., Adegbaju, Muyiwa S., Okoh, Olayinka S., Babalola, Asegunloluwa E., Grytsay, Anastasia, Braimah, Olubumi A., Akinyemi, Mabel O., Thomas, Bolaji N.   +7 more
openaire   +3 more sources

Prediction of Small Molecule Inhibitors Targeting the Severe Acute Respiratory Syndrome Coronavirus-2 RNA-dependent RNA Polymerase [PDF]

ACS Omega, 2020
The current COVID-19 outbreak warrants the design and development of novel anti-COVID therapeutics. Using a combination of bioinformatics and computational tools, we modelled the 3D structure of the RdRp (RNA-dependent RNA polymerase) of SARS-CoV2 (severe acute respiratory syndrome coronavirus-2) and predicted its probable GTP binding pocket in the ...
Ahmad, Mohammed, Dwivedy, Abhisek, Mariadasse, Richard, Tiwari, Satish, Kar, Deepsikha, Jeyakanthan, Jeyaraman, Biswal, Bichitra K.   +6 more
openaire   +3 more sources

Coronavirus Cell-Associated RNA-Dependent RNA Polymerase [PDF]

, 1981
Infectious, single-stranded, nonsegmented, polyadenylated, genomic RNA has been demonstrated for the avian infectious bronchitis virus (Lomniczi and Kennedy, 1977; Schochetman et al., 1977), the mouse hepatitis virus (Lai and Stohlman, 1978; Wege et al., 1978), and the transmissible gastroenteritis virus (TGEV) of swine (Brian et al., 1980), three ...
D E, Dennis, D A, Brian
openaire   +2 more sources

Biochemical characterization of a recombinant SARS coronavirus nsp12 RNA-dependent RNA polymerase capable of copying viral RNA templates [PDF]

Archives of Virology, 2012
The severe acute respiratory syndrome coronavirus (SARS-CoV) RNA genome is replicated by a virus-encoded RNA replicase, the key component of which is the nonstructural protein 12 (nsp12). In this report, we describe the biochemical properties of a full-length recombinant SARS-CoV nsp12 RNA-dependent RNA polymerase (RdRp) capable of copying viral RNA ...
Ahn, Dae-Gyun, Choi, Jin-Kyu, Taylor, Deborah R., Oh, Jong-Won   +3 more
openaire   +2 more sources