Rapid Identification of Superior Endogenous Signal Peptides for Heterologous Protein Secretion by Corynebacterium glutamicum Through Modular Cloning and Automation. [PDF]
Microb BiotechnolA modular cloning approach combined with an automated high‐throughput platform enabled rapid identification of superior endogenous signal peptides for the secretion of proteins of interest (POIs), such as industrial enzymes, by Corynebacterium glutamicum. This streamlined workflow accelerates discovery and development of efficient bacterial systems for
Matamouros S +4 more
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Metabolic Engineering for Valorization of Agri- and Aqua-Culture Sidestreams for Production of Nitrogenous Compounds by Corynebacterium glutamicum [PDF]
Frontiers in Microbiology, 2022 Corynebacterium glutamicum is used for the million-ton-scale production of amino acids. Valorization of sidestreams from agri- and aqua-culture has focused on the production of biofuels and carboxylic acids.
Volker F. Wendisch +2 more
doaj +2 more sources
Efficient Multiplex Gene Repression by CRISPR-dCpf1 in Corynebacterium glutamicum [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 Corynebacterium glutamicum is an important workhorse for industrial production of diversiform bioproducts. Multiplex control of metabolic pathway genes is crucial for maximizing biosynthesis of desired products.
Mingyue Li +9 more
doaj +2 more sources
Production of the Antimicrobial Roseoflavin With Genetically Engineered Corynebacterium glutamicum [PDF]
Microbial BiotechnologyThe global rise of drug‐resistant bacterial infections underscores an urgent demand for novel antimicrobial agents. Roseoflavin, a naturally occurring riboflavin analog, has emerged as a promising candidate for drug development.
Luciana Fernandes Brito +4 more
doaj +2 more sources
Engineering Dual-Input Glucose- and Temperature-Sensitive Lysis Circuits in Corynebacterium glutamicum for Efficient Intracellular Product Recovery [PDF]
MicroorganismsCorynebacterium glutamicum is a versatile microbial cell factory, but efficient recovery of intracellular macromolecules remains a major challenge. In this study, we engineered environmentally controllable lysis systems to enable programmable product ...
Ziyu Ye +5 more
doaj +2 more sources
Systems metabolic engineering of Corynebacterium glutamicum for efficient l-tryptophan production [PDF]
Synthetic and Systems BiotechnologyCorynebacterium glutamicum is a versatile industrial microorganism for producing various amino acids. However, there have been no reports of well-defined C. glutamicum strains capable of hyperproducing l-tryptophan.
Yufei Dong, Zhen Chen
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Safety and efficacy of a feed additive consisting of L-valine produced using <i>Corynebacterium glutamicum</i> KCCM 80366 for all animal species (CJ Europe GmbH). [PDF]
EFSA JEFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) +23 more
europepmc +3 more sources
Synthetic biology tools for engineering Corynebacterium glutamicum
Computational and Structural Biotechnology Journal, 2023 Corynebacterium glutamicum is a promising organism for the industrial production of amino acids, fuels, and various value-added chemicals. From the whole genome sequence release, C. glutamicum has been valuable in the field of industrial microbiology and
Gi Yeon Kim +5 more
doaj +1 more source
Nature Communications, 2022 Corynebacterium glutamicum is a major workhorse in industrial biomanufacturing of amino acids. Here, the authors employ CRISPR-assisted rational flux-tuning and CRISPRi screening of a L-proline exporter to covert a wild-type C.
Jiao Liu +15 more
doaj +1 more source
Iron Delivery through Membrane Vesicles in Corynebacterium glutamicum
Microbiology Spectrum, 2023 Bacterial cells form and release membrane vesicles (MVs) originating from cellular membranes. In recent years, many biological functions of bacterial MVs have been identified.
Kayuki Kawashima +3 more
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