Results 41 to 50 of about 2,861,963 (268)
Bio-Protocol, 2020 Saturation mutagenesis is a fundamental enabling technology for protein engineering and epitope mapping. Nicking mutagenesis (NM) allows the user to rapidly construct libraries of all possible single mutations in a target protein sequence from plasmid ...
Paul Steiner +2 more
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Инфекция и иммунитет, 2019 Chronic hepatitis B is a severe liver disease caused by persistent infection of hepatitis B virus in human hepatocytes. Chronic hepatitis B is one of the most common diseases in the world. According to recent estimations, more than 250 million people are
A. P. Kostyusheva +8 more
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Nucleic acids and protein synthesis in cancer cell mitochondria. I. Nucleic acids in rat hepatoma mitochondria [PDF]
, 1967 The contents of nucleic acids in rat liver and hepatoma mitochondria and the physico-chemical properties on DNA's isolated from these mitochondria were comparatively investigated. The results are briefly summarized as follows. 1.
Inaba, Kozo
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The accessory subunit of mitochondrial DNA polymerase {gamma} determines the DNA content of mitochondrial nucleoids in human cultured cells [PDF]
, 2009 The accessory subunit of mitochondrial DNA polymerase γ, POLGβ, functions as a processivity factor in vitro. Here we show POLGβ has additional roles in mitochondrial DNA metabolism.
H. Sembongi +38 more
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Monomer and multimer covalently closed circular forms of Rous sarcoma virus DNA [PDF]
Journal of Virology, 1979 Covalently closed circular molecules of viral DNA synthesized in virus-infected cells are composed mainly of monomers sedimenting at 22 to 27S in neutral sucrose gradients. These monomers are detected by annealing with complementary DNA or transfection assay. However, 11% of the infectious circles sediment faster than monomers.
G, Goubin, M, Hill
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The cohesin ring concatenates sister DNA molecules [PDF]
, 2008 Sister chromatid cohesion, which is essential for mitosis, is mediated by a multi-subunit protein complex called cohesin whose Scc1, Smc1, and Smc3 subunits form a tripartite ring structure.
Arumugam, P +9 more
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A new method for the purification and identification of covalently closed circular DNA molecules
Nucleic Acids Research, 1978 A new technique has been developed for the rapid isolation of covalently closed circular DNA molecules. The procedure is a selective extraction based on differences in the partitioning of covalently closed circular DNA molecules and noncovalently closed species between phenol and water at acid pH and low ionic strength.
M, Zasloff, G D, Ginder, G, Felsenfeld
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Quantatitative assays for covalently closed circular DNA of hepatitis B virus [PDF]
Chinese Science Bulletin, 2020 Chronic infection of hepatitis B virus (HBV) is still one of the public health concerns affecting human health. Although the prophylactic HBV vaccines was introduced over three decades ago, the total number of chronic hepatitis B (CHB) infection still accounts for 3.5% (257 million) of the world’s population, with approximately 800000 annual deaths ...
Tianshu Shi +3 more
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Recent advances in the study of hepatitis B virus covalently closed circular DNA [PDF]
Virologica Sinica, 2017 Chronic hepatitis B infection is caused by hepatitis B virus (HBV) and a total cure is yet to be achieved. The viral covalently closed circular DNA (cccDNA) is the key to establish a persistent infection within hepatocytes. Current antiviral strategies have no effect on the pre-existing cccDNA reservoir.
Mengying Ji, Kanghong Hu
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A simple large-scale method for separating closed circular form DNA by gel electrophoresis [PDF]
, 1978 The covalently closed form of circular duplex SV40 DNA was separated from the open and linear form of SV40 DNA by agarose gel electrophoresis in a large-scale gel system.
Oda, Takuzo +3 more
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