Results 1 to 10 of about 277,742 (368)
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells [PDF]
Science, 2013 Improving Whole-Genome Screens Improved methods are needed for the knockout of individual genes in genome-scale functional screens. Wang et al. (p. 80, published online 12 December) and Shalem et al. (p.
Ophir Shalem +10 more
openalex +2 more sources
Physical Model of the Immune Response of Bacteria Against Bacteriophage Through the Adaptive CRISPR-Cas Immune System [PDF]
Phys. Biol. 10 (2013) 025004, 2014 Bacteria and archaea have evolved an adaptive, heritable immune system that recognizes and protects against viruses or plasmids. This system, known as the CRISPR-Cas system, allows the host to recognize and incorporate short foreign DNA or RNA sequences, called `spacers' into its CRISPR system.
Pu Han +3 more
arxiv +3 more sources
mBio, 2023 Forkhead-associated (FHA) domain proteins specifically recognize phosphorylated threonine via the FHA domain and are involved in signal transduction in various processes especially DNA damage response (DDR) and cell cycle regulation in eukaryotes ...
Zhichao Jiang +9 more
doaj +1 more source
A Well-Conserved Archaeal B-Family Polymerase Functions as an Extender in Translesion Synthesis
mBio, 2022 B-family DNA polymerases (PolBs) of different groups are widespread in Archaea, and different PolBs often coexist in the same organism. Many of these PolB enzymes remain to be investigated.
Xu Feng +9 more
doaj +1 more source
Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins
Scientific Reports, 2021 Efforts to improve CRISPR-Cas9 genome editing systems for lower off-target effects are mostly at the cost of its robust on-target efficiency. To enhance both accuracy and efficiency, we created chimeric SpyCas9 proteins fused with the 5′-to-3 ...
Jongjin Park +11 more
doaj +1 more source
A review on genome editing by CRISPR-Cas9 technique for cancer treatment [PDF]
World Cancer Research Journal, 2020 In this review, we summarize CRISPR-Cas9 system-based gene modification for the therapeutic treatment of cancer. Cancer is a group of diseases involving anomalous cell growth with the potential to invade or blow out to other parts of the body, which is ...
M. Niuz Morshed Khan +3 more
doaj +1 more source
Heterologous autoimmunity and prokaryotic immune defense [PDF]
Current Biology 2022, 2021 Some prokaryotes possess CRISPR-Cas systems that provide adaptive immunity to viruses guided by DNA segments called spacers acquired from invading phage. However, the patchy incidence and limited memory breadth of CRISPR-Cas systems suggest that their fitness benefits are offset by costs.
arxiv +1 more source
Enzymatic Switching Between Archaeal DNA Polymerases Facilitates Abasic Site Bypass
Frontiers in Microbiology, 2021 Abasic sites are among the most abundant DNA lesions encountered by cells. Their replication requires actions of specialized DNA polymerases. Herein, two archaeal specialized DNA polymerases were examined for their capability to perform translesion DNA ...
Xu Feng +10 more
doaj +1 more source
Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing [PDF]
Arab Universities Journal of Agricultural Sciences, 2023 Worldwide, potato (Solanum tuberosum L.) is one of the most essential agro-economically food crops. To ensure its presence throughout the year for food processors and to extend its shelf life, it should be stored in cold temperatures.
Sally Hassan +5 more
doaj +1 more source
mLife, 2023 Kinase, putative Endopeptidase, and Other Proteins of Small size (KEOPS) is a multisubunit protein complex conserved in eukaryotes and archaea.
Pengju Wu +8 more
doaj +1 more source