Results 1 to 10 of about 442,156 (406)

Physical Model of the Immune Response of Bacteria Against Bacteriophage Through the Adaptive CRISPR-Cas Immune System [PDF]

, 2013
Bacteria and archaea have evolved an adaptive, heritable immune system that recognizes and protects against viruses or plasmids. This system, known as the CRISPR-Cas system, allows the host to recognize and incorporate short foreign DNA or RNA sequences,
Barrick, Jeffrey E., Deem, Michael W., Han, Pu, Niestemski, Liang Ren   +3 more
core   +3 more sources

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells [PDF]

Science, 2014
Ophir Shalem, Neville E. Sanjana, E. Hartenian, Xi Shi, David A. Scott, T. Mikkelsen, D. Heckl, B. Ebert, D. Root, John G Doench, Feng Zhang   +10 more
semanticscholar   +2 more sources

An updated evolutionary classification of CRISPR–Cas systems

Nature Reviews Microbiology, 2015
Kira S Makarova, Yuri I Wolf, Omer S Alkhnbashi   +2 more
exaly   +2 more sources

The FHA domain protein ArnA functions as a global DNA damage response repressor in the hyperthermophilic archaeon Saccharolobus islandicus

mBio, 2023
Forkhead-associated (FHA) domain proteins specifically recognize phosphorylated threonine via the FHA domain and are involved in signal transduction in various processes especially DNA damage response (DDR) and cell cycle regulation in eukaryotes ...
Zhichao Jiang, Zijia Lin, Qi Gan, Pengju Wu, Xuemei Zhang, Yuanxi Xiao, Qunxin She, Jinfeng Ni, Yulong Shen, Qihong Huang   +9 more
doaj   +1 more source

Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins

Scientific Reports, 2021
Efforts to improve CRISPR-Cas9 genome editing systems for lower off-target effects are mostly at the cost of its robust on-target efficiency. To enhance both accuracy and efficiency, we created chimeric SpyCas9 proteins fused with the 5′-to-3 ...
Jongjin Park, Jiyoung Yoon, Daekee Kwon, Mi-Jung Han, Sunmee Choi, Slki Park, Junghyuk Lee, Kiwook Lee, Jaehwan Lee, Seunghee Lee, Kyung-Sun Kang, Sunghwa Choe   +11 more
doaj   +1 more source

A Well-Conserved Archaeal B-Family Polymerase Functions as an Extender in Translesion Synthesis

mBio, 2022
B-family DNA polymerases (PolBs) of different groups are widespread in Archaea, and different PolBs often coexist in the same organism. Many of these PolB enzymes remain to be investigated.
Xu Feng, Baochang Zhang, Zhe Gao, Ruyi Xu, Xiaotong Liu, Sonoko Ishino, Mingxia Feng, Yulong Shen, Yoshizumi Ishino, Qunxin She   +9 more
doaj   +1 more source

The canonical single-stranded DNA-binding protein is not an essential replication factor but an RNA chaperon in Saccharolobus islandicus

iScience, 2023
Summary: Single-stranded DNA-binding proteins (SSBs) have been regarded as indispensable replication factors. Herein, we report that the genes encoding the canonical SSB (SisSSB) and the non-canonical SSB (SisDBP) in Saccharolobus islandicus REY15A are ...
Yuanxi Xiao, Zhichao Jiang, Mengqi Zhang, Xuemei Zhang, Qi Gan, Yunfeng Yang, Pengju Wu, Xu Feng, Jinfeng Ni, Xiuzhu Dong, Qunxin She, Qihong Huang, Yulong Shen   +12 more
doaj   +1 more source

Decreasing of Monosaccharide Contents Resulted From Cold- Induced Sweetening in Tetraploid Potato (Solanum tuberosum L.) Using Genome Editing [PDF]

Arab Universities Journal of Agricultural Sciences, 2023
Worldwide, potato (Solanum tuberosum L.) is one of the most essential agro-economically food crops. To ensure its presence throughout the year for food processors and to extend its shelf life, it should be stored in cold temperatures.
Sally Hassan, Mohamed Abdel-Salam, ayman hanfy, Haytham Abd-Elhalim, Nouh Ahmed, Emad Metry   +5 more
doaj   +1 more source

Enzymatic Switching Between Archaeal DNA Polymerases Facilitates Abasic Site Bypass

Frontiers in Microbiology, 2021
Abasic sites are among the most abundant DNA lesions encountered by cells. Their replication requires actions of specialized DNA polymerases. Herein, two archaeal specialized DNA polymerases were examined for their capability to perform translesion DNA ...
Xu Feng, Baochang Zhang, Ruyi Xu, Zhe Gao, Xiaotong Liu, Guanhua Yuan, Sonoko Ishino, Mingxia Feng, Yulong Shen, Yoshizumi Ishino, Qunxin She   +10 more
doaj   +1 more source

The archaeal KEOPS complex possesses a functional Gon7 homolog and has an essential function independent of the cellular t6A modification level

mLife, 2023
Kinase, putative Endopeptidase, and Other Proteins of Small size (KEOPS) is a multisubunit protein complex conserved in eukaryotes and archaea.
Pengju Wu, Qi Gan, Xuemei Zhang, Yunfeng Yang, Yuanxi Xiao, Qunxin She, Jinfeng Ni, Qihong Huang, Yulong Shen   +8 more
doaj   +1 more source