Results 91 to 100 of about 83,279 (184)

Viral Infection‐Inspired Autonomous Detection of Fusion‐Competent Viruses for Screening and Environmental Surveillance

Advanced Materials, Volume 38, Issue 40, 17 July 2026.
Inspired by viral entry mechanisms, the FUSION assay enables autonomous detection of respiratory viruses via membrane fusion–triggered CRISPR‐Cas13a activation. VEACON selectively fuses with fusion‐competent viruses, triggering fluorescence within confined vesicles.
Jae Chul Park, Yidam Song, Hyun‐Woo Choi, Jaeuk Sung, Harin Jin, WonSeok Gwak, Kirim Yoo, Soohwang Lee, Jongeon Park, Jeongmin Kim, Hye‐Jun Jo, Jahyun Koo, Youngdo Jeong, Kwan Hyi Lee, Seung‐Jung Kee, Hojun Kim   +15 more
wiley   +1 more source

Foldback-crRNA-Enhanced CRISPR/Cas13a System (FCECas13a) Enables Direct Detection of Ultrashort sncRNA

, 2023
The CRISPR/Cas13a system has promising applications in clinical small noncoding RNA (sncRNA) detection because it is free from the interference of genomic DNA.
Qianling Zhang, Xinyao Yang (1482325), Siyuan Luo, Yizhen Liu (1970200), Xueji Zhang, Xinyao Yang, Xueji Zhang (392238), Conghui Liu, Yong Chen (109188), Siyuan Luo (4490908), Conghui Liu (542714), Yizhen Liu, Yibin Zhang (1426579), Qianling Zhang (5877506), Yong Chen, Yibin Zhang   +15 more
core   +1 more source

CRISPR‐Enabled Point‐of‐Care Diagnostics for Early Cancer Detection

Aggregate, Volume 7, Issue 7, July 2026.
This review summarizes recent advances in CRISPR‐enabled point‐of‐care diagnostics for early cancer detection, covering Cas‐based recognition mechanisms, portable engineering platforms, and biomarker applications. It further highlights current challenges and future opportunities for translating sensitive, affordable, and accessible CRISPR diagnostics ...
Yuwei Pan, Qihang Xu, Carla Spatola Rossi, Zhugen Yang   +3 more
wiley   +1 more source

Potential role of a CRISPR-Cas-activated toxin-antitoxin system in bacterial immunity

Nature Communications
CRISPR-Cas and toxin-antitoxin systems can serve as antiviral defense mechanisms in prokaryotes. In typical toxin-antitoxin systems, toxin activation can limit phage propagation by inducing growth arrest or reduced cellular fitness, while the antitoxin ...
Jiyun Chen, Linglong Huang, Hong Chen, Xueyan Li, Xiaofeng Lin, Chenmin Guo, Xi Liu, Guowei Fu, Ying Chen, Liang Liu   +9 more
doaj   +1 more source

Approaching Gold‐Standard Sensitivity in a Portable and Versatile Gold Nanoprisms Thermoplasmonic Platform for Lateral Flow Detection of Biomolecules

Small, Volume 22, Issue 41, 22 July 2026.
This study presents a plasmonic thermal lateral flow (ThermoLFA) biosensor for ultrasensitive, early detection of biomolecules. By combining plasmonic gold nanoprisms (AuNPrs) and near‐infrared (NIR) laser activation, it enhances signal output beyond conventional LFAs.
Carlos Cuestas Ayllón, Alba Martín Barreiro, África García Barrientos, María Moros, Valeria Grazú Bonavía, Jesús Martínez de la Fuente   +5 more
wiley   +1 more source

Multiple nucleic acid cleavage modes in divergent type III CRISPR systems [PDF]

, 2016
CRISPR-Cas is an RNA-guided adaptive immune system that protects bacteria and archaea from invading nucleic acids. Type III systems (Cmr, Csm) have been shown to cleave RNA targets in vitro and some are capable of transcription-dependent DNA targeting ...
Zhang, Jing, White, Malcolm Frederick, Tello, Agnes, Liu, Huanting, Graham, Shirley   +4 more
core   +1 more source

Table_1_A Highly Sensitive and Specific Detection Method for Mycobacterium tuberculosis Fluoroquinolone Resistance Mutations Utilizing the CRISPR-Cas13a System.docx

, 2022
ObjectivesCRISPR-Cas13a system-based nucleic acid detection methods are reported to have rapid and sensitive DNA detection. However, the screening strategy for crRNAs that enables CRISPR-Cas13a single-base resolution DNA detection of human pathogens ...
Jiandong Yang (196004), Miaomiao Han (838543), Quanxin Long (714921), Huizhi Zheng (12540391), Keli Qian (10534357), Yaoqin Lu (12540394), Xiaoxia Cao (12331721), Haijun Deng (492257), Xiaopeng Bai (2643814), Tiwei Fu (6294884), Ailong Huang (196688), Panqi Gao (12540388), Yuliang Liu (522424), Yuan Hu (275971)   +13 more
core   +1 more source

Based on CRISPR-Cas13a system, to establish a rapid visual detection method for avian influenza viruses

Frontiers in Veterinary Science
To rapidly, specifically, and sensitively detect avian influenza virus (AIV), this research established a visual detection method of recombinase-aided amplification (RAA) based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and ...
Zongshu Zhang, Chunguang Wang, Xi Chen, Zichuang Zhang, Guoqiang Shi, Xianghe Zhai, Tie Zhang   +6 more
doaj   +1 more source

Synergistic intravesical instillation for bladder cancer: CRISPR-Cas13a and fenbendazole combination therapy

Journal of Experimental & Clinical Cancer Research
Background CRISPR-Cas13a is renowned for its precise and potent RNA editing capabilities in cancer therapy. While various material systems have demonstrated efficacy in supporting CRISPR-Cas13a to execute cellular functions in vitro efficiently and ...
Mingkang Liang, Yongqiang Wang, Lisha Liu, Dashi Deng, Zeqin Yan, Lida Feng, Chenfan Kong, Chenchen Li, Yuqing Li, Guangzhi Li   +9 more
doaj   +1 more source

Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9 [PDF]

, 2015
CRISPR/Cas9 technologies have been employed for genome editing to achieve gene knockouts and knock-ins in somatic cells. Similarly, certain endogenous genes have been tagged with fluorescent proteins. Often, the detection of tagged proteins requires high
Rojas-Fernandez, Alejandro, Hay, Ronald T., Macartney, Thomas, Herhaus, Lina, Sapkota, Gopal P., Lachaud, Christophe   +5 more
core   +1 more source