Results 101 to 110 of about 83,279 (184)

Field-deployable porcine epidemic diarrhea virus diagnostics utilizing CRISPR-Cas13a

Virulence
Porcine epidemic diarrhoea virus (PEDV), a pathogenic microorganism that induces epidemic diarrhoea in swine, causes substantial economic damage to swine-farming nations.
Yuanyuan Wang, Dalin He, Weihua Li, Yaqin Dong, Linlin Fang, Deju Liu, Yi Tang, Shaobo Xiao   +7 more
doaj   +1 more source

CRISPR/Cas13a-based genome editing for establishing the detection method of H9N2 subtype avian influenza virus

Poultry Science
: Avian influenza virus (AIV) subtype H9N2 has significantly threatened the poultry business in recent years by having become the predominant subtype in flocks of chickens, ducks, and pigeons.
Sha-Sha Chen, Yong-Lei Yang, Hong-Yun Wang, Tian-Kui Guo, Riaz-M Azeem, Chun-Wei Shi, Gui-Lian Yang, Hai-Bin Huang, Yan-Long Jiang, Jian-Zhong Wang, Xin Cao, Nan Wang, Yan Zeng, Wen-Tao Yang, Chun-Feng Wang   +14 more
doaj   +1 more source

CRISPR/Cas13a Signal Amplification Linked Immunosorbent Assay for Femtomolar Protein Detection

, 2019
The enzyme-linked immunosorbent assay (ELISA) is a basic technique used in analytical and clinical investigations. However, conventional ELISA is still not sensitive enough to detect ultralow concentrations of biomarkers for the early diagnosis of cancer,
Erhu Xiong (4379821), Po Wang (3430508), Qian Chen (18883), Xiaoming Zhou (308488), Tian Tian (170088)   +4 more
core   +1 more source

A Multifunction Lipid-Based CRISPR-Cas13a Genetic Circuit Delivery System for Bladder Cancer Gene Therapy

, 2019
The treatment of bladder cancer has recently shown minimal progress. Gene therapy mediated by CRISPR provides a new option for bladder cancer treatment.
Weiren Huang (150992), Yuchen Liu (112600), Lisa Liu (453938), Xuemei Li (232384), Yikun Huang (8276166), Jing Fan (149601)   +5 more
core   +1 more source

RPA-CRISPR-Cas13a-assisted detection method of transmissible gastroenteritis virus

Frontiers in Veterinary Science
Background and aimTransmissible gastroenteritis virus (TGEV) is a highly contagious gastrointestinal virus that causes diarrhea, vomiting, anorexia, dehydration, and weight loss in piglets. In clinical practice, it often occurs in mixed infections with other pathogens, and is therefore difficult to diagnose and prevent. It mainly harms piglets of about
Haoyu Wang, Zhimeng Cheng, Zhimeng Cheng, Ran Luo, Qiyue Yang, Yongping Zeng, Yijun Yang, Yuankun Chen, Wenting Li, Wenting Li, Xiao Liu, Xiao Liu   +11 more
openaire   +3 more sources

Rapid and sensitive diagnosis of live Mycobacterium tuberculosis using clustered regularly interspaced short palindromic repeat‐Cas13a point‐of‐care RNA testing

View
Mycobacterium tuberculosis (MTB) is the causal pathogen of tuberculosis (TB). Rapid and accurate detection of live MTB is important for transmission control and patient treatment.
Yu Wang, Huihuang Lin, Anqi Yang, Jiaming Huang, Weicong Ren, Jiajun Dong, Shaojie Wang, Wenxue Xu, Yu Pang, Jieming Qu, Jia Liu   +10 more
doaj   +1 more source

An extraction-free, lyophilized one-pot RAA-CRISPR assay for point-of-care testing of Haemophilus influenzae

Journal of Clinical Microbiology
Timely and accurate diagnosis is essential for the effective treatment of Haemophilus influenzae (HI) infection. Notably, a rapid point‐of-care testing (POCT) method for the diagnosis of HI DNA is still lacking.
Yaling Cao, Junwen Wang, Zihao Fan, Ling Xu, Zhenzhen Pan, Yinkang Mo, Qiwen Yang, Jing Huang, Feng Ren   +8 more
doaj   +1 more source

Table_5_Composition and Diversity of CRISPR-Cas13a Systems in the Genus Leptotrichia.XLSX

, 2019
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas13a, previously known as CRISPR-C2c2, is the most recently identified RNA-guided RNA-targeting CRISPR-Cas system that has the unique characteristics of both targeted and collateral ...
Yumi Nakada (8104565), Yoshifumi Aiba (5948150), Tanit Boonsiri (5948159), Bintao Cui (5948165), Longzhu Cui (5948168), Yusuke Sato’o (4803960), Teppei Sasahara (159779), Yusuke Taki (8104556), Kotaro Kiga (693099), Moriyuki Kawauchi (2820625), Kanate Thitiananpakorn (5948162), Shinya Watanabe (3092), Aa Haeruman Azam (8104562), Xin-Ee Tan (5948153), Fen-Yu Li (8104559)   +14 more
core   +1 more source

An Amplified Luminescent Proximity Homogeneous Assay-Implemented CRISPR/Cas13a System for Noncoding RNA Detection

The CRISPR-Cas13a detection system is an RNA detection method developed in recent years, which precisely identifies the target RNA sequence to be detected by base complementary pairing.
Zheng Wang, Jingxuan Xu, Xiumei Zhou, Yaofan Lu, Jingxia Han, Yuan Qin, Bing Gu, Zhenya Cheng, Biao Huang, Jingyuan Ma, Xueqin Zhao, Fuyuan Ma   +11 more
core   +1 more source

CRISPR/Cas Enzyme Catalysis in Liquid–Liquid Phase‐Separated Systems

Advanced Science
The clustered regularly interspaced palindromic repeats (CRISPR) /CRISPR‐associated proteins (Cas) system is the immune system in bacteria and archaea and has been extensively applied as a critical tool in bioengineering.
Yaqin Zhang, Jianai Chen, Zhina Wu, Chenfei Zhao, Rui Wang, Zhiping Li, Jiasi Wang, Di Wang   +7 more
doaj   +1 more source