Results 221 to 230 of about 248,807 (309)

Consequences of CRISPR-Cas9-Mediated Stromelysin-1 Knockout in Pancreatic Islet Microvascular Endothelial Cells. [PDF]

J Cell Mol Med
Wang B, Liu W, Li Y, Ouyang Q, Wang Y, Xu X, Li B, Xiu R, Zhang X, Liu M.   +9 more
europepmc   +1 more source

Directed Evolution Improves the Catalytic Efficiency of APEX2‐Mediated Proximity‐Dependent RNA Labeling

Advanced Science, EarlyView.
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley   +1 more source

Ablation of PKCα Phosphorylation by CRISPR-Cas9 Base Editing Rescues Heart Failure. [PDF]

Circ Res
Tadokoro T, Li H, Gan P, Xu Z, Tan W, Alzhanov D, Sánchez-Ortiz E, McAnally JR, Guo L, Xu L, Ruan P, Liu N, Olson EN.   +12 more
europepmc   +1 more source

TopCas: Topology‐Gated Cas12a via DNA‐RNA Chimeric Circular crRNA for Amplification‐Free Nucleic Acid Detection and Conditional Gene Editing

Advanced Science, EarlyView.
The TopCas platform utilizes an engineered DNA‐RNA chimeric circular crRNA to establish topology‐gated control over CRISPR‐Cas12a activity, enabling target‐triggered self‐catalytic amplification (preamplification‐free). This system seamlessly integrates highly sensitive molecular diagnostics with conditionally controlled gene editing, demonstrating ...
Shun Zhang, Wen Sun, Ting Xiao, Yali Wang, Xianlan Wu, Huiyou Chen, Ming Chen, Jun Zhang   +7 more
wiley   +1 more source

CRISPR/Cas9-mediated B2m knockout paves the way for allogeneic basal cell transplantation. [PDF]

Respir Res
Chen H, Luo W, Ma N, Li S.
europepmc   +1 more source

Covalent Reprogramming of Kinase Binders to Modulate Protein Abundance

Advanced Science, EarlyView.
Electrophilic remodeling of a broad‐spectrum kinase binder reveals how subtle chemical changes reprogram protein fate. An acrylamide analog of a multi‐kinase binder selectively stabilizes Aurora kinase A (AURKA) by suppressing its ubiquitination, while a short‐linker variant converts this stabilizer into a degrader.
Chen Mozes, Xiaokang Jin, Miguel A. Campos, Chen Zhou, Xiaoyu Zhang   +4 more
wiley   +1 more source

High genotoxicity of CRISPR/Cas9 versus limited efficacy of CRISPRi in chicken primordial germ cells. [PDF]

Poult Sci
Zhang C, Wang M, He B, Yang X, Li XZ.
europepmc   +1 more source

Palmitic Acid Promotes Antiviral Innate Immunity via ZDHHC20‐Mediated CMPK2 Palmitoylation

Advanced Science, EarlyView.
Metabolites have important functions in innate immune activation and regulation. Wang et al. uncover metabolic regulation of antiviral immunity through CMPK2 palmitoylation, which regulates CMPK2 mitochondrial localization and is promoted by ZDHHC20 but reversed by PPT1, inhibition of which antagonizes viral infection in mice.
Yujia Wang, Zenghui Cui, Yunkai Zhang, Zhiqing Li, Xuetao Cao   +4 more
wiley   +1 more source

CRISPR-Cas9-based gene editing as a proof-of-concept approach in an inborn error of immunity caused by a DCLRE1C variant. [PDF]

Immunol Res
Duran T, Karaselek MA, Dagdelen B, Kuccukturk S, Guner SN, Keles S, Reisli I.   +6 more
europepmc   +1 more source