Results 241 to 250 of about 259,520 (336)

AND logic-gated CRISPR/Cas9 and hybridization chain reaction system for precise ctDNA detection. [PDF]

J Nanobiotechnology
Ji T, Zhang Y, Wang Y, Yuan K, Wang M, Ye J, Zhang H, Zhang N, Zhang H.   +8 more
europepmc   +1 more source

Palmitoylation‐Mediated Ubiquitination of SRPK1 Regulates Ferroptosis in High‐Fat‐Induced Erectile Dysfunction

Advanced Science, EarlyView.
Elevated exogenous palmitic acid promotes the S‐palmitoylation of SRPK1 in endothelial cells, a dynamic process governed by ZDHHC24 and APT1. This post‐translational modification strengthens the interaction between SRPK1 and the E3 ubiquitin ligase MIB1, thereby facilitating the proteasomal degradation of SRPK1.
Xiao‐Hui Tan, Ke‐Fan Li, Yi‐Ming Yuan, Man‐Cheng Xia, Fang‐Zhou Zhao, Hong‐Gang Ying, Zhuo Zhou, Peng‐Chao Gao, Guo‐Qing Xie, Xue‐Song Li, Hui Jiang, Rui‐Li Guan   +11 more
wiley   +1 more source

Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation. [PDF]

Nat Commun
Srinivasan R, Sun T, Sandles A, Wu D, Wang L, Patel H, Pabalate R, Bader M, Heidersbach A, Ho C, Xie S, Ng A, Haley B.   +12 more
europepmc   +1 more source

Peptide dendrimers transfecting CRISPR/Cas9 plasmid DNA: optimization and mechanism

Susanna Zamolo, Elena Zakharova, Lise Boursinhac, Florian Hollfelder, Tamis Darbre, Jean‐Louis Reymond   +5 more
openalex   +2 more sources

Quantitative Proteomics and CRISPR/Cas9 Editing Reveal UPR‐Mediated Control of Immunoglobulin Homeostasis in Hybridomas

Advanced Science, EarlyView.
BCR sequencing and subclone analysis correlated immunoglobulin (Ig) chain loss in dysfunctional hybridomas with disrupted monoclonal antibody homeostasis. Proteomics‐guided CRISPR/Cas9 editing revealed that the unfolded protein response (UPR) regulates aberrant Ig synthesis.
Rubing Zou, Xinying Lu, Ying Liu, Peiyu Yang, Shuo Sun, Yihua Liu, Yinyuan Mo, Guonian Zhu, Jae Seong Lee, Yirong Guo   +9 more
wiley   +1 more source

PDIA3 Inhibition Facilitates Sensitivity of IKE‐Induced Ferroptosis via STAT3/LCN2 Axis to Improve Glioblastoma Therapy

Advanced Science, EarlyView.
In this manuscript, protein disulfide isomerase A3 (PDIA3) is identified as a key factor mediating the susceptibility of ferroptosis in GBM. Inhibition of PDIA3 enhances IKE or cystine starvation‐induced ferroptosis in GBM cells by resulting in the accumulation of lipid peroxidation and a reduction in GSH level.
Jie Zhang, Wei Wang, Xin Liu, Peifen Lu, Xinjing Liu, Qiucheng Nie, Siyuan Xin, Hong Li, Donglin Yu, Xinyue Zhang, Kailong Li, Xiaomin Han, Shuping Zhang, Wei Chong, Lili Sun, Wei Li, Tao Xin, Jin Jiao, Qiang Ma, Yiju Wei   +19 more
wiley   +1 more source

Identification of novel regulators of LINE-1 expression via CRISPR/Cas9 screening. [PDF]

Mob DNA
Oksuz O, Chu C, Arisdakessian C, Diao L, Zaller D, Long KK, Keilhack H, Knutson S.   +7 more
europepmc   +1 more source

RICE CRISPR: Rapidly increased cut ends by an exonuclease Cas9 fusion in zebrafish

, 2017
Thomas P. Clements, Bhavna Tandon, Hendrik A. Lintel, Joseph H. McCarty, Daniel S. Wagner   +4 more
openalex   +2 more sources

Uncoupling Metastasis and Epithelial‐to‐Mesenchymal Transition in sgP19/kRAS‐Driven Spontaneous Metastatic Liver Tumor Model

Advanced Science, EarlyView.
The article first reported that a murine mixed typical intrahepatic cholangiocarcinoma (iCCA)/sarcomatoid iCCA liver tumor model with 100% incidence of spontaneous extrahepatic metastasis and confirmed the occurrence of in mouse iCCA. Most importantly, EMT induced by the TGF‐β/ZEB1 axis does not influence tumor development or distant metastasis in this
Jingwen Wang, Zijing Xu, Lei Xu, Lishan Wang, Jiahao Geng, Xue Wang, Meng Xu, Daphne Superville, Melissa Reeves, Matthias Evert, Diego F. Calvisi, Xin Chen, Xinhua Song   +12 more
wiley   +1 more source

In vivo CRISPR/Cas9 screens identify new regulators of B cell activation and plasma cell differentiation. [PDF]

J Exp Med
Calderón L, Schäfer M, Rončević M, Rauschmeier R, Jaritz M, Schwickert TA, Sun Q, Pauli A, Zuber J, Busslinger M.   +9 more
europepmc   +1 more source