Results 241 to 250 of about 245,728 (306)

An improved CRISPR-Cas9 protein-based method for knocking out insect Sf9 cell genes. [PDF]

Appl Microbiol Biotechnol
Graça M, Virgolini N, Correia R, Escandell J, Roldão A.   +4 more
europepmc   +1 more source

Multi‐Targeting Non‐Specific Genome Engineering in Bacteria

Advanced Science, EarlyView.
In this study, we provide the first case to use the multi‐targeting integrase (MTI) systems in bacteria and develop a host‐independent generalizable approach, MNGE (Multi‐targeting Non‐specific Genome Engineering), for multi‐copy and random integration of metabolic genes or pathways in both Gram‐positive and Gram‐negative bacteria, which will ...
Runze Sun, Ruixiang You, Yiwen Zhou, Huiyan He, Wenfang Wang, Xudong Qu, Yinhua Lu, Lei Li   +7 more
wiley   +1 more source

Integrative Analyses Identify a cGAS‐STING Pathway‐Driven Signature With Context‐Dependent Roles in Systemic Lupus Erythematosus

Advanced Science, EarlyView.
Zhang et al. identify M7core, a critical cGAS‐STING pathway‐driven gene signature that is activated in most lupus patients’ blood and links to lupus disease severity, lymphopenia, and lupus nephritis. They further reveal the diagnostic and pathogenic characteristics of M7core and emphasize the importance of assessing pathway activity before initiating ...
Lele Zhang, Ming‐Ju Amy Lyu, Ze Hong, Jing Sun, Lan Guo, Jiameng Hu, Munire Maimaiti, Chenhui Li, Jinyi Song, Xiang Zhang, Huansha Yu, Chen Wang, Xuan Wang, Haiyang Hu   +13 more
wiley   +1 more source

Coronavirus Nsp3 Hijacks CLTC to Modulate Autophagosome Nucleation for Promoting DMV Formation and Viral Replication

Advanced Science, EarlyView.
In wild‐type cells, FIPV infection recruits CLTC to nsp3, facilitates DMV biogenesis and block autophagic flux to promote viral replication. CLTC knockout impairs autophagosome nucleation by reducing Beclin1–ATG14 complex expression. This disrupts the formation of autophagic precursor membranes, thereby preventing their hijacking by nsp3 for DMV ...
Juan Xu, Hang Li, Peng Liu, Zhe Jiao, Ding Zhang, Zhelin Su, Sai Niu, Jintao Zhang, Yuejun Shi, Guiqing Peng   +9 more
wiley   +1 more source

A narrative review of precision and ethical considerations in cardiovascular health: CRISPR-Cas9, telemedicine, and lifestyle interventions. [PDF]

Front Public Health
Lu Z, Bukhari STH, Azeem M, Tariq N, Shabbir MAB.   +4 more
europepmc   +1 more source

Discovering CRISPR/Cas9

, 2016
Prieto Pascual, Cristina, Universitat Autònoma de Barcelona. Facultat de Biociències   +1 more
openaire   +1 more source

Bio-orthogonal chemistry-based strategy to Turn-OFF CRISPR-Cas9 activity in solution and live cells. [PDF]

NAR Mol Med
Pandit B, Vangaveti S, Sentre JF, McClain I, Fuchs G, Royzen M.   +5 more
europepmc   +1 more source

CRISPR-Cas9-Generated TXNDC15 c.560delA Homozygous Mouse Model Exhibits Meckel-Gruber Syndrome Phenotype. [PDF]

Genesis
Liu Y, Wang H, Chen L, Wu X, Xu Z, Huang Q, Zhang H, Cao X, Liang X, Zhong X, Luo C.   +10 more
europepmc   +1 more source

Improved CRISPR/Cas9 off-target prediction with DNABERT and epigenetic features. [PDF]

PLoS One
Kimata K, Satou K.
europepmc   +1 more source

Allele-selective disruption of pathogenic VWF variants in type 2 von Willebrand disease using CRISPR/Cas9. [PDF]

Blood Adv
Bär I, Groten SA, Barraclough A, Bürgisser PE, van Kwawegen C, Lenting PJ, van Moort I, Eikenboom JCJ, Leebeek FWG, Voorberg J, van den Biggelaar M, Bierings R.   +11 more
europepmc   +1 more source