Results 261 to 270 of about 216,936 (304)
Some of the next articles are maybe not open access.
Nature Reviews Molecular Cell Biology, 2020
The discovery of the CRISPR–Cas9 system by Emmanuelle Charpentier and Jennifer Doudna awarded a 2020 Nobel Prize in Chemistry.
Paulina Strzyz, Strzyz Paulina
exaly +3 more sources
The discovery of the CRISPR–Cas9 system by Emmanuelle Charpentier and Jennifer Doudna awarded a 2020 Nobel Prize in Chemistry.
Paulina Strzyz, Strzyz Paulina
exaly +3 more sources
CRISPR–Cas9 Structures and Mechanisms
Annual Review of Biophysics, 2017Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific double-stranded breaks in target DNA.
Fuguo Jiang, Jennifer A Doudna
exaly +3 more sources
2023
CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and ease of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells using the CRISPR/Cas9 system.
Izuho, Hatada +2 more
openaire +3 more sources
CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and ease of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells using the CRISPR/Cas9 system.
Izuho, Hatada +2 more
openaire +3 more sources
2017
CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
Izuho, Hatada, Takuro, Horii
openaire +3 more sources
CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
Izuho, Hatada, Takuro, Horii
openaire +3 more sources
ACM SIGGRAPH 2018 Posters, 2018
CRISPR/Cas9-NHEJ: Action in the Nucleus (2017) is derived from an interdisciplinary creative process. This paper discusses the creation of this 210° scientific visualization, the usage of data from the worldwide Protein Data Bank, and the audio-visual presentation in an interactive dome setup.
Martina R. Fröschl, Alfred Vendl
openaire +1 more source
CRISPR/Cas9-NHEJ: Action in the Nucleus (2017) is derived from an interdisciplinary creative process. This paper discusses the creation of this 210° scientific visualization, the usage of data from the worldwide Protein Data Bank, and the audio-visual presentation in an interactive dome setup.
Martina R. Fröschl, Alfred Vendl
openaire +1 more source
Liposomal delivery of CRISPR/Cas9
Cancer Gene Therapy, 2019Liposomes are one of the most widely investigated carriers for CRISPR/Cas9 delivery. The surface properties of liposomal carriers, including the surface charge, PEGylation, and ligand modification can significantly affect the gene silencing efficiency. Three barriers of systemic CRISPR/Cas9 delivery (long blood circulation, efficient tumor penetration,
Shuai, Zhen, Xu, Li
openaire +2 more sources
CRISPR-Cas9 in cancer therapeutics
2021Cancer is a disease mainly caused by an accumulation of mutations in cells. Consequently, correcting those genetic aberrations could be a potential treatment strategy. The traditional route for cancer drug development is tedious, laborious, and time-consuming.
openaire +2 more sources
Nature Cell Biology, 2019
MicroRNAs (miRNAs) repress target mRNAs, often with exquisite tissue specificity. Wang et al. exploit the specific expression of miRNAs to regulate guide production for Cas9. Their method enables novel strategies to simultaneously measure the activity of multiple miRNAs and restrict Cas9 binding or genome editing to precisely defined cell types.
Karina, Jouravleva, Phillip D, Zamore
openaire +2 more sources
MicroRNAs (miRNAs) repress target mRNAs, often with exquisite tissue specificity. Wang et al. exploit the specific expression of miRNAs to regulate guide production for Cas9. Their method enables novel strategies to simultaneously measure the activity of multiple miRNAs and restrict Cas9 binding or genome editing to precisely defined cell types.
Karina, Jouravleva, Phillip D, Zamore
openaire +2 more sources