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Nature Reviews Molecular Cell Biology, 2020
The discovery of the CRISPR–Cas9 system by Emmanuelle Charpentier and Jennifer Doudna awarded a 2020 Nobel Prize in Chemistry.
Paulina Strzyz, Strzyz Paulina
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The discovery of the CRISPR–Cas9 system by Emmanuelle Charpentier and Jennifer Doudna awarded a 2020 Nobel Prize in Chemistry.
Paulina Strzyz, Strzyz Paulina
exaly +3 more sources
CRISPR–Cas9 Structures and Mechanisms
Annual Review of Biophysics, 2017Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific double-stranded breaks in target DNA.
Jennifer Doudna, Fuguo Jiang
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ACM SIGGRAPH 2018 Posters, 2018
CRISPR/Cas9-NHEJ: Action in the Nucleus (2017) is derived from an interdisciplinary creative process. This paper discusses the creation of this 210° scientific visualization, the usage of data from the worldwide Protein Data Bank, and the audio-visual presentation in an interactive dome setup.
Martina R. Fröschl, Alfred Vendl
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CRISPR/Cas9-NHEJ: Action in the Nucleus (2017) is derived from an interdisciplinary creative process. This paper discusses the creation of this 210° scientific visualization, the usage of data from the worldwide Protein Data Bank, and the audio-visual presentation in an interactive dome setup.
Martina R. Fröschl, Alfred Vendl
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Liposomal delivery of CRISPR/Cas9
Cancer Gene Therapy, 2019Liposomes are one of the most widely investigated carriers for CRISPR/Cas9 delivery. The surface properties of liposomal carriers, including the surface charge, PEGylation, and ligand modification can significantly affect the gene silencing efficiency. Three barriers of systemic CRISPR/Cas9 delivery (long blood circulation, efficient tumor penetration,
Shuai, Zhen, Xu, Li
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CRISPR-Cas9 in cancer therapeutics
2021Cancer is a disease mainly caused by an accumulation of mutations in cells. Consequently, correcting those genetic aberrations could be a potential treatment strategy. The traditional route for cancer drug development is tedious, laborious, and time-consuming.
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Nature Cell Biology, 2019
MicroRNAs (miRNAs) repress target mRNAs, often with exquisite tissue specificity. Wang et al. exploit the specific expression of miRNAs to regulate guide production for Cas9. Their method enables novel strategies to simultaneously measure the activity of multiple miRNAs and restrict Cas9 binding or genome editing to precisely defined cell types.
Karina, Jouravleva, Phillip D, Zamore
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MicroRNAs (miRNAs) repress target mRNAs, often with exquisite tissue specificity. Wang et al. exploit the specific expression of miRNAs to regulate guide production for Cas9. Their method enables novel strategies to simultaneously measure the activity of multiple miRNAs and restrict Cas9 binding or genome editing to precisely defined cell types.
Karina, Jouravleva, Phillip D, Zamore
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CRISPR Cas9 − Licensing the unlicensable
Journal of Biotechnology, 2018A new gene engineering technology has recently made it through the media, not only because of its technical advantages, but also because it is in the focus of an epic patent battle between two academic institutions. The technology bears the cryptic name "CRISPR Cas9", and allows the manipulation of genes (so called "gene editing") with so far unseen ...
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CRISPR/Cas9 for Mutagenesis in Rice
2018CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9) provides a workhorse for genome editing biotechnology. CRISPR/Cas9 tailored for enabling genome editing has been extensively interrogated and widely utilized for precise genomic alterations in eukaryotic organisms including in plant species.
Si Nian, Char, Riqing, Li, Bing, Yang
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Catching CRISPR-Cas9 in Action
Journal of Chemical Theory and ComputationCRISPR-Cas9 has revolutionized genome editing, yet its structural dynamics and functional properties remain incompletely understood, partly due to limited atomic-level characterization of its active conformation with a full R-loop. Capitalizing on recent advances in Cas9 structural determination, we constructed a catalytic-state Cas9 model bound to a ...
Yingjie Chen +5 more
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CRISPR/Cas9 in the Chicken Embryo
2017Genome editing is driving a revolution in the biomedical sciences that carries the promise for future treatments of genetic diseases. The CRISPR/Cas9 system of RNA-guided genome editing has been successfully applied to modify the genome of a wide spectrum of organisms.
Valérie, Morin +2 more
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