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CRISPR Cas9 Worksheet

2023
Students can use this worksheet to collect information regarding the process of CRISPR ...
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Liposomal delivery of CRISPR/Cas9

Cancer Gene Therapy, 2019
Liposomes are one of the most widely investigated carriers for CRISPR/Cas9 delivery. The surface properties of liposomal carriers, including the surface charge, PEGylation, and ligand modification can significantly affect the gene silencing efficiency. Three barriers of systemic CRISPR/Cas9 delivery (long blood circulation, efficient tumor penetration,
Shuai, Zhen, Xu, Li
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Cascading CRISPR–Cas9 genome edits

Nature Reviews Genetics, 2021
In this study in Molecular Cell, Clarke et al. describe a system that enables multiple Cas9-mediated genome edits to be introduced into cells in a defined, sequential order.
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CRISPR–Cas9 Structures and Mechanisms

Annual Review of Biophysics, 2017
Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific double-stranded breaks in target DNA.
Fuguo, Jiang, Jennifer A, Doudna
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CRISPR-Cas9 in cancer therapeutics

2021
Cancer is a disease mainly caused by an accumulation of mutations in cells. Consequently, correcting those genetic aberrations could be a potential treatment strategy. The traditional route for cancer drug development is tedious, laborious, and time-consuming.
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CRISPR Cas9 − Licensing the unlicensable

Journal of Biotechnology, 2018
A new gene engineering technology has recently made it through the media, not only because of its technical advantages, but also because it is in the focus of an epic patent battle between two academic institutions. The technology bears the cryptic name "CRISPR Cas9", and allows the manipulation of genes (so called "gene editing") with so far unseen ...
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CRISPR/Cas9

2022
Sylvia Uzochukwu, Arinze S. Okoli
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Controlling CRISPR-Cas9 Gene Editing

New England Journal of Medicine, 2019
Cracking Down on CRISPR-Cas9 Off-target DNA editing by the CRISPR-Cas9 ribonucleoprotein nuclease in the experimental treatment of genetic disease is a safety concern.
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