Results 51 to 60 of about 245,728 (306)

CRISPR/Cas9-Based Antiviral Strategy: Current Status and the Potential Challenge

Molecules, 2019
From its unexpected discovery as a bacterial adaptive immune system to its countless applications as one of the most versatile gene-editing tools, the CRISPR/Cas9 system has revolutionized every field of life science.
Choongho Lee
doaj   +1 more source

Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing

Frontiers in Genetics, 2021
The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises
Li Duan, Li Duan, Li Duan, Kan Ouyang, Xiao Xu, Xiao Xu, Limei Xu, Limei Xu, Caining Wen, Caining Wen, Xiaoying Zhou, Xiaoying Zhou, Zhuan Qin, Zhuan Qin, Zhiyi Xu, Zhiyi Xu, Wei Sun, Yujie Liang   +17 more
doaj   +1 more source

Spontaneous Embedding of DNA Mismatches Within the RNA:DNA Hybrid of CRISPR-Cas9. [PDF]

, 2020
CRISPR-Cas9 is the forefront technology for editing the genome. In this system, the Cas9 protein is programmed with guide RNAs to process DNA sequences that match the guide RNA forming an RNA:DNA hybrid structure.
Hsu, Rohaine V, Medrano, Marco A, Mitchell, Brandon P, Narkhede, Yogesh B, Palermo, Giulia, Zewde, Nehemiah T   +5 more
core  

Sequence-Based Mapping and Genome Editing Reveal Mutations in Stickleback Hps5 Cause Oculocutaneous Albinism and the casper Phenotype. [PDF]

, 2017
Here, we present and characterize the spontaneous X-linked recessive mutation casper, which causes oculocutaneous albinism in threespine sticklebacks (Gasterosteus aculeatus).
Hart, James C, Miller, Craig T
core   +2 more sources

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

High-capacity adenovector delivery of forced CRISPR-Cas9 heterodimers fosters precise chromosomal deletions in human cells

Molecular Therapy: Nucleic Acids, 2023
Genome editing based on dual CRISPR-Cas9 complexes (multiplexes) permits removing specific genomic sequences in living cells leveraging research on functional genomics and genetic therapies.
Francesca Tasca, Marcella Brescia, Jin Liu, Josephine M. Janssen, Kamel Mamchaoui, Manuel A.F.V. Gonçalves   +5 more
doaj   +1 more source

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source

Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering

BMC Plant Biology, 2020
Traditionally, generation of new plants with improved or desirable features has relied on laborious and time-consuming breeding techniques. Genome-editing technologies have led to a new era of genome engineering, enabling an effective, precise, and rapid
Naoki Wada, Risa Ueta, Yuriko Osakabe, Keishi Osakabe   +3 more
doaj   +1 more source

The Advance of CRISPR-Cas9-Based and NIR/CRISPR-Cas9-Based Imaging System

Frontiers in Chemistry, 2021
The study of different genes, chromosomes and the spatiotemporal relationship between them is of great significance in the field of biomedicine. CRISPR-Cas9 has become the most widely used gene editing tool due to its excellent targeting ability.
Huanhuan Qiao, Jieting Wu, Xiaodong Zhang, Jian Luo, Jian Luo, Hao Wang, Dong Ming   +6 more
doaj   +1 more source

Effective genome editing and identification of a regiospecific gallic acid 4-O-glycosyltransferase in pomegranate (Punica granatum L.). [PDF]

, 2019
Pomegranate (Punica granatum L.) trees are woody perennials that bear colorful and nutritious fruits rich in phenolic metabolites, e.g., hydrolyzable tannins (HTs) and flavonoids.
Chang, Lijing, Tian, Li, Wu, Sheng
core   +1 more source