Results 231 to 240 of about 713,183 (311)

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

, 2014
Ryan E. Cobb, Yajie Wang, Huimin Zhao
openalex   +1 more source

CRISPR-Cas Systems and Genome Editing: Beginning the Era of CRISPR/Cas Therapies for Humans

International Journal of Molecular Sciences
Harnessing of CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated genes) systems for detection, chemical modification, and sequence editing of nucleic acids dramatically changed many fields of fundamental science, biotechnology, and biomedicine [...]
openaire   +2 more sources

The Encapsulation Strategies for Targeted Delivery of Probiotics in Preventing and Treating Colorectal Cancer: A Review

Advanced Science, EarlyView.
This work summarizes the efficacy and mechanisms of probiotics in CRC treatment, providing a comprehensive overview of various encapsulation methods such as microcapsules, hydrogels, single‐cell nanocoatings, and nanofibers for targeted probiotic delivery to inflammatory sites, with particular emphasis on the potential advantages of emerging single ...
Hao Zhong, Jin Jiang, Muhammad Hussain, Haoxuan Zhang, Ling Chen, Rongfa Guan   +5 more
wiley   +1 more source

MISSA 2.0: an updated synthetic biology toolbox for assembly of orthogonal CRISPR/Cas systems [PDF]

, 2017
Haiyan Zhang, Xinghui Wang, Dong Li, Zhiping Wang, Bing Liu, Jie Lv, Xing Hui-li, Chunyan Han, Xuechen Wang, Qijun Chen   +9 more
openalex   +1 more source

Efficient Generation of Megakaryocyte Progenitors and Platelets From HSPCs via JAK2/STAT3 Signaling

Advanced Science, EarlyView.
This research presents a novel strategy employing the VGM cocktail, which combines HES7 overexpression with HDAC inhibitor and GABA, to enhance platelet production from hematopoietic stem and progenitor cells by promoting megakaryocyte progenitor (MkP) generation.
Huicong Liu, Lingna Wang, Jiaqing Liu, Haitao Yuan, Kaiqing Zhang, Yun Qiu, Fangfang Zhu   +6 more
wiley   +1 more source

Priming in a permissive type I-C CRISPR–Cas system reveals distinct dynamics of spacer acquisition and loss [PDF]

, 2017
Chitong Rao, Denny Chin, Alexander W. Ensminger   +2 more
openalex   +1 more source

Mechanical Load‐Induced Upregulation of Talin2 through Non‐Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis

Advanced Science, EarlyView.
Facet joint osteoarthritis (FJOA), prevalent in the elderly, involves Talin2 upregulation promoting ECM degradation while suppressing synthesis. Mechanical load stabilizes Talin2 via OTUB1‐mediated non‐canonical deubiquitination, driving CCL2 expression that recruits immune cells, exacerbating inflammatory joint degeneration.
Yizhen Huang, Heng Sun, Haojie Chen, Xiangpeng Wang, Junduo Zhao, Yang Jiao, Hongyi Zhou, Haoyu Cai, Jiafeng Dai, Xuan Huang, Weiyun Chen, Jianxiong Shen   +11 more
wiley   +1 more source

Oligonucleotide separation techniques for purification and analysis: What can we learn for today's tasks?

ELECTROPHORESIS, Volume 43, Issue 23-24, Page 2402-2427, December 2022., 2022
Abstract Nucleic acids are the blueprint of life. They are not only the construction plan of the single cell or higher associations of them, but also necessary for function, communication and regulation. Due to the pandemic, the attention shifted in particular to their therapeutic potential as a vaccine. As pharmaceutical oligonucleotides are unique in
Robert Minkner, Jirayu Boonyakida, Enoch Y. Park, Hermann Wätzig   +3 more
wiley   +1 more source

Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system

, 2014
Wataru Fujii, Asuka Onuma, Koji Sugiura, Kunihiko Naito   +3 more
openalex   +1 more source

Mathematical modelling of CRISPR-Cas system effects on biofilm formation [PDF]

, 2017
Qasim Ali, Lindi M. Wahl
openalex   +1 more source