Results 31 to 40 of about 717,978 (310)

CRISPR-Cas Systems Reduced to a Minimum [PDF]

Molecular Cell, 2019
In two recent studies in Molecular Cell, Wright et al. (2019) report complete spacer integration by a Cas1 mini-integrase and Edraki et al. (2019) describe accurate genome editing by a small Cas9 ortholog with less stringent PAM requirements.
Sebastian N. Kieper, Cristóbal Almendros, Stan J. J. Brouns, Stan J. J. Brouns   +3 more
openaire   +4 more sources

A global comprehensive study of the distribution of type I-E and type I-E* CRISPR-Cas systems in Klebsiella pneumoniae

Indian Journal of Community Medicine, 2023
Background: The CRISPR-Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) systems are the short DNA sequences and RNA-dependent nuclease involved in the adaptive immunity in bacteria and archaea. The type of CRISPR-
Anand Babu Kannadasan, Venil Naranan Sumantran, Rama Vaidyanathan   +2 more
doaj   +1 more source

Evolution and classification of the CRISPR–Cas systems [PDF]

Nature Reviews Microbiology, 2011
The CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) modules are adaptive immunity systems that are present in many archaea and bacteria. These defence systems are encoded by operons that have an extraordinarily diverse architecture and a high rate of evolution for both the cas genes and the unique ...
Yuri I. Wolf, Philippe Horvath, Alexander F. Yakunin, Kira S. Makarova, Rodolphe Barrangou, John van der Oost, Stan J. J. Brouns, Eugene V. Koonin, Daniel H. Haft, Francisco J. M. Mojica, Emmanuelle Charpentier, Sylvain Moineau   +11 more
openaire   +4 more sources

CRISPR-Cas systems in Proteus mirabilis

Infection, Genetics and Evolution, 2021
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is a bacterial defense mechanism against bacteriophages composed of two different parts: the CRISPR array and the Cas genes. The spacer acquisition is done by the adaptation module consisting of the hallmark Cas1 Cas2 proteins, which inserts new spacers into the CRISPR array.
Fallah, M.S., Mohebbi, A., Yasaghi, M., Ghaemi, E.A.   +3 more
openaire   +3 more sources

Functional Features and Current Applications of the RNA‐Targeting Type VI CRISPR‐Cas Systems

Advanced Science, 2021
CRISPR‐Cas systems are a form of prokaryotic adaptive immunity that employs RNA‐guided endonucleases (Cas effectors) to cleave foreign genetic elements.
Vanja Perčulija, Jinying Lin, Bo Zhang, Songying Ouyang   +3 more
doaj   +1 more source

Nanocarriers: A novel strategy for the delivery of CRISPR/Cas systems

Frontiers in Chemistry, 2022
In recent decades, clustered regularly interspaced short palindromic repeat/CRISPR-associated protein (CRISPR/Cas) has become one of the most promising genome-editing tools for therapeutic purposes in biomedical and medical applications.
Faranak Hejabi, Mohammad Sadegh Abbaszadeh, Shirinsadat Taji, Andrew O’Neill, Fatemeh Farjadian, Mohammad Doroudian   +5 more
doaj   +1 more source

Origins and evolution of CRISPR-Cas systems [PDF]

Philosophical Transactions of the Royal Society B: Biological Sciences, 2019
CRISPR-Cas, the bacterial and archaeal adaptive immunity systems, encompass a complex machinery that integrates fragments of foreign nucleic acids, mostly from mobile genetic elements (MGE), into CRISPR arrays embedded in microbial genomes. Transcripts of the inserted segments (spacers) are employed by CRISPR-Cas systems as guide (g)RNAs for ...
Eugene V. Koonin, Kira S. Makarova
openaire   +3 more sources

Viral Diversity Threshold for Adaptive Immunity in Prokaryotes

mBio, 2012
Bacteria and archaea face continual onslaughts of rapidly diversifying viruses and plasmids. Many prokaryotes maintain adaptive immune systems known as clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (Cas ...
Ariel D. Weinberger, Yuri I. Wolf, Alexander E. Lobkovsky, Michael S. Gilmore, Eugene V. Koonin   +4 more
doaj   +1 more source

Epidemiological and evolutionary consequences of different types of CRISPR-Cas systems.

PLoS Computational Biology, 2022
Bacteria have adaptive immunity against viruses (phages) in the form of CRISPR-Cas immune systems. Currently, 6 types of CRISPR-Cas systems are known and the molecular study of three of these has revealed important molecular differences. It is unknown if
Hélène Chabas, Viktor Müller, Sebastian Bonhoeffer, Roland R Regoes   +3 more
doaj   +1 more source

Strategies for High-Efficiency Mutation Using the CRISPR/Cas System

Frontiers in Cell and Developmental Biology, 2022
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated systems have revolutionized traditional gene-editing tools and are a significant tool for ameliorating gene defects.
Shuying Feng, Zilong Wang, Aifang Li, Xin Xie, Junjie Liu, Shuxuan Li, Yalan Li, Baiyan Wang, Lina Hu, Lianhe Yang, Tao Guo   +10 more
doaj   +1 more source