Results 31 to 40 of about 58,663 (324)
Clades of huge phages from across Earth's ecosystems. [PDF]
, 2020 Bacteriophages typically have small genomes1 and depend on their bacterial hosts for replication2. Here we sequenced DNA from diverse ecosystems and found hundreds of phage genomes with lengths of more than 200 kilobases (kb), including a genome of ...
Al-Shayeb, Basem +44 more
core +2 more sources
Application of different types of CRISPR/Cas-based systems in bacteria
Microbial Cell Factories, 2020 As important genome editing tools, CRISPR/Cas systems, especially those based on type II Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria.
Zhenquan Liu +4 more
doaj +1 more source
Optimization of CRISPR–Cas system for clinical cancer therapy
Bioengineering & Translational Medicine, 2023 Cancer is a genetic disease caused by alterations in genome and epigenome and is one of the leading causes for death worldwide. The exploration of disease development and therapeutic strategies at the genetic level have become the key to the treatment of
Xiang Meng +7 more
doaj +1 more source
Indian Journal of Community Medicine, 2023 Background: The CRISPR-Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) systems are the short DNA sequences and RNA-dependent nuclease involved in the adaptive immunity in bacteria and archaea. The type of CRISPR-
Anand Babu Kannadasan +2 more
doaj +1 more source
CRISPR-Cas Systems Reduced to a Minimum [PDF]
Molecular Cell, 2019 In two recent studies in Molecular Cell, Wright et al. (2019) report complete spacer integration by a Cas1 mini-integrase and Edraki et al. (2019) describe accurate genome editing by a small Cas9 ortholog with less stringent PAM requirements.
Sebastian N. Kieper +3 more
openaire +4 more sources
CRISPR-Cas systems in Proteus mirabilis
Infection, Genetics and Evolution, 2021 The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) is a bacterial defense mechanism against bacteriophages composed of two different parts: the CRISPR array and the Cas genes. The spacer acquisition is done by the adaptation module consisting of the hallmark Cas1 Cas2 proteins, which inserts new spacers into the CRISPR array.
Ezzat Allah Ghaemi +3 more
openaire +3 more sources
The Application of CRISPR/Cas Systems for Antiviral Therapy [PDF]
Frontiers in Genome Editing, 2021 As CRISPR/Cas systems have been refined over time, there has been an effort to apply them to real world problems, such as developing sequence-targeted antiviral therapies. Viruses pose a major threat to humans and new tools are urgently needed to combat these rapidly mutating pathogens.
Baddeley, H, Isalan, M
openaire +5 more sources
Characterization of CRISPR-Cas Systems in Serratia marcescens Isolated from Rhynchophorus ferrugineus (Olivier, 1790) (Coleoptera: Curculionidae) [PDF]
, 2019 The CRISPR-Cas adaptive immune system has been attracting increasing scientific interest for biological functions and biotechnological applications. Data on the Serratia marcescens system are scarce.
Calia, Carla +7 more
core +1 more source
Origins and evolution of CRISPR-Cas systems [PDF]
Philosophical Transactions of the Royal Society B: Biological Sciences, 2019 CRISPR-Cas, the bacterial and archaeal adaptive immunity systems, encompass a complex machinery that integrates fragments of foreign nucleic acids, mostly from mobile genetic elements (MGE), into CRISPR arrays embedded in microbial genomes. Transcripts of the inserted segments (spacers) are employed by CRISPR-Cas systems as guide (g)RNAs for ...
Eugene V. Koonin, Kira S. Makarova
openaire +3 more sources
A Newly Discovered Bordetella Species Carries a Transcriptionally Active CRISPR-Cas with a Small Cas9 Endonuclease [PDF]
, 2015 Background Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria.
Dudley, Edward G. +7 more
core +2 more sources