Results 31 to 40 of about 277,742 (368)

CRISPR-based diagnostics [PDF]

Nature Biomedical Engineering, 2021
The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic acids are among the most sensitive and specific, yet most such assays require costly equipment and trained personnel.
Michael M. Kaminski, Omar O. Abudayyeh, J. Gootenberg, Feng Zhang, J. Collins   +4 more
semanticscholar   +6 more sources

CRISPR as a Diagnostic Tool [PDF]

Biomolecules, 2021
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system has recently gained growing attention as a diagnostic tool due to its capability of specific gene targeting. It consists of Cas enzymes and a guide RNA (gRNA) that can cleave the target DNA or RNA based on the sequence of the gRNA, making it an attractive genetic engineering ...
Seohyun Kim, Hye Ran Koh, Sangmin Ji
openaire   +4 more sources

A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

National Science Review, 2022
The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed.
Zhi Chen, Jingfeng Li, Tianzhong Li, Taojian Fan, Changle Meng, Chao Li, Jianlong Kang, Luxiao Chai, Yabin Hao, Yuxuan Tang, O. Al-Hartomy, S. Wageh, Abdullah G. Al‐Sehemi, Zhiguang Luo, Jiangtian Yu, Y. Shao, Defa Li, Shuai Feng, William J. Liu, Yaqing He, Xiao-Ping Ma, Zhongjian Xie, Han Zhang   +22 more
semanticscholar   +1 more source

Allelic Variants of CRISPR/Cas9 Induced Mutation in an Inositol Trisphosphate 5/6 Kinase Gene Manifest Different Phenotypes in Barley

Plants, 2020
Inositol trisphosphate 5/6 kinases (ITPK) constitute a small group of enzymes participating in the sequential phosphorylation of inositol phosphate to inositol hexakisphosphate (IP6), which is a major storage form of phosphate in cereal grains.
Tomáš Vlčko, Ludmila Ohnoutková
doaj   +1 more source

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering [PDF]

Cell, 2013
Haoyi Wang, Hui Yang, Chikdu Shivalila, Meelad M. Dawlaty, Albert W. Cheng, Feng Zhang, Rudolf Jaenisch   +6 more
openalex   +2 more sources

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

Nature Biotechnology, 2015
CRISPR-Cas9–based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off ...
John G Doench, Nicoló Fusi, Meagan E. Sullender, Mudra Hegde, Emma Vaimberg, Katherine F. Donovan, Ian Smith, Z. Tothova, Craig B. Wilen, R. Orchard, H. Virgin, J. Listgarten, D. Root   +12 more
semanticscholar   +1 more source

A human rights analysis of clustered regularly interspaced short palindromic repeats germline-editing for disease prevention

Journal of Public Health and Primary Care, 2020
Our purpose is to discuss ethical concerns with disease prevention applications of CRISPR germline editing using a human rights approach. If applied towards furthering health, these tools may aid to prevent diseases, thereby improving health and reduce ...
Lance Garrett Shaver, Amit Sundly, Abdullah Omar Saif   +2 more
doaj   +1 more source

Nucleic acid detection with CRISPR-Cas13a/C2c2

Science, 2017
Sensitive and specific CRISPR diagnostics Methods are needed that can easily detect nucleic acids that signal the presence of pathogens, even at very low levels. Gootenberg et al.
J. Gootenberg, Omar O. Abudayyeh, Jeong Wook Lee, Patrick Essletzbichler, Aaron J. Dy, J. Joung, Vanessa Verdine, Nina M. Donghia, Nichole M. Daringer, C. A. Freije, C. Myhrvold, R. Bhattacharyya, J. Livny, A. Regev, E. Koonin, D. Hung, Pardis C Sabeti, J. Collins, Feng Zhang   +18 more
semanticscholar   +1 more source

A Highly Sensitive CRISPR‐Empowered Surface Plasmon Resonance Sensor for Diagnosis of Inherited Diseases with Femtomolar‐Level Real‐Time Quantification

Advancement of science, 2022
The clustered regularly interspaced short palindromic repeats (CRISPR) molecular system has emerged as a promising technology for the detection of nucleic acids.
Fei Zheng, Zhi Chen, Jingfeng Li, Ruige Wu, Bin Zhang, Guohui Nie, Zhongjian Xie, Han Zhang   +7 more
semanticscholar   +1 more source

Cloning-free CRISPR

Stem Cell Reports, 2015
We present self-cloning CRISPR/Cas9 (scCRISPR), a technology that allows for CRISPR/Cas9-mediated genomic mutation and site-specific knockin transgene creation within several hours by circumventing the need to clone a site-specific single-guide RNA (sgRNA) or knockin homology construct for each target locus.
Mandana Arbab, Richard I. Sherwood, Tatsunori Hashimoto, Sharanya Srinivasan, Sharanya Srinivasan, Niels Geijsen   +5 more
openaire   +9 more sources