Results 31 to 40 of about 349,258 (340)
Stem Cell Reports, 2015 We present self-cloning CRISPR/Cas9 (scCRISPR), a technology that allows for CRISPR/Cas9-mediated genomic mutation and site-specific knockin transgene creation within several hours by circumventing the need to clone a site-specific single-guide RNA (sgRNA) or knockin homology construct for each target locus.
Mandana Arbab +5 more
openaire +9 more sources
Journal of Public Health and Primary Care, 2020 Our purpose is to discuss ethical concerns with disease prevention applications of CRISPR germline editing using a human rights approach. If applied towards furthering health, these tools may aid to prevent diseases, thereby improving health and reduce ...
Lance Garrett Shaver +2 more
doaj +1 more source
Physical Model of the Immune Response of Bacteria Against Bacteriophage Through the Adaptive CRISPR-Cas Immune System [PDF]
, 2013 Bacteria and archaea have evolved an adaptive, heritable immune system that recognizes and protects against viruses or plasmids. This system, known as the CRISPR-Cas system, allows the host to recognize and incorporate short foreign DNA or RNA sequences,
Barrick, Jeffrey E. +3 more
core +1 more source
CRISPR/Cas9-induced (CTG⋅CAG)n repeat instability in the myotonic dystrophy type 1 locus: implications for therapeutic genome editing [PDF]
, 2017 Myotonic dystrophy type 1 (DM1) is caused by (CTG⋅CAG)n-repeat expansion within the DMPK gene and thought to be mediated by a toxic RNA gain of function.
André, Laurène M. +11 more
core +3 more sources
Highly Efficient Site-Specific Mutagenesis in Malaria Mosquitoes Using CRISPR. [PDF]
, 2018 Anopheles mosquitoes transmit at least 200 million annual malaria infections worldwide. Despite considerable genomic resources, mechanistic understanding of biological processes in Anopheles has been hampered by a lack of tools for reverse genetics. Here,
Akbari, Omar S +2 more
core +7 more sources
Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]
, 2015 Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas +3 more
core +2 more sources
CRISPR/Cas9‐mediated genome editing: from basic research to translational medicine [PDF]
, 2020 The recent development of the CRISPR/Cas9 system as an efficient and accessible programmable genome-editing tool has revolutionized basic science research. CRISPR/Cas9 system-based technologies have armed researchers with new powerful tools to unveil the
Ferreira, B I +2 more
core +1 more source
Anti‐CRISPRs: The natural inhibitors for CRISPR‐Cas systems [PDF]
Animal Models and Experimental Medicine, 2019 AbstractCRISPR (clustered regularly interspaced short palindromic repeats)‐Cas (CRISPR associated protein) systems serve as the adaptive immune system by which prokaryotes defend themselves against phages. It has also been developed into a series of powerful gene‐editing tools. As the natural inhibitors of CRISPR‐Cas systems, anti‐CRISPRs (Acrs) can be
Fei Zhang, Guoxu Song, Yong Tian
openaire +4 more sources
The precise magic of CRISPR [PDF]
FEBS Open Bio, 2021 In this issue of FEBS Open Bio, Shen Li et al., in the laboratory of Hector L. Franco (University of North Carolina), provide a proof‐of‐principle solution for correcting all copies of a gene in the widely used MCF7 breast cancer cell line. The gene for the FOXA1 pioneer transcription factor is localised on chromosome 14, which is present at least 4–5 ...
openaire +3 more sources
iScience, 2023 Summary: Single-stranded DNA-binding proteins (SSBs) have been regarded as indispensable replication factors. Herein, we report that the genes encoding the canonical SSB (SisSSB) and the non-canonical SSB (SisDBP) in Saccharolobus islandicus REY15A are ...
Yuanxi Xiao +12 more
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