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Book Review: “The New Microbiology: From Microbiomes to CRISPR”
Annals of Clinical Microbiology, 2022 At the time when owing to COVID-19 pandemic, the public is more interested in infectious diseases and pathogens than in any other period, a book titled “The New Microbiology: From Microbiomes to CRISPR” by the renowned microbiologist Pascale Cossart was ...
Jung Oak Kang
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Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]
, 2015 Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas +3 more
core +2 more sources
CRISPR as a Diagnostic Tool [PDF]
Biomolecules, 2021 Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system has recently gained growing attention as a diagnostic tool due to its capability of specific gene targeting. It consists of Cas enzymes and a guide RNA (gRNA) that can cleave the target DNA or RNA based on the sequence of the gRNA, making it an attractive genetic engineering ...
Seohyun Kim, Hye Ran Koh, Sangmin Ji
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Desarrollo de nuevos diseños vegetales: Mejora genética vs transformación genética y edición génica
Magna Scientia UCEVA, 2021 El objetivo de esta reflexión es realizar un estudio comparativo sobre la eficacia, eficiencia técnica y costo de las tecnologías más importantes para el desarrollo de nuevos diseños vegetales, incluyendo la mejora genética, la transformación genética y ...
Pedro Martínez Gómez
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Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
Nature Biotechnology, 2015 CRISPR-Cas9–based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off ...
John G Doench +12 more
semanticscholar +1 more source
CRISPR-based diagnostics [PDF]
Nature Biomedical Engineering, 2021 The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic acids are among the most sensitive and specific, yet most such assays require costly equipment and trained personnel.
Feng Zhang +5 more
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CRISPR-Cas Systems for Detection of Nucleic Acids: PCR Empire now has a Vigorous Rival!
Translational Research in Urology, 2021 Rapid and accurate detection of nucleic acids is essential in many fields of modern biology and medical diagnosis of pathogens. PCR has some disadvantages for performing this task, and therefore demand for an alternative is highly appreciated.
Roham Deyhimfar
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BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]
, 2016 Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien +7 more
core +2 more sources
Current trends and new methods of detection of SARS-CoV-2 infection [PDF]
Cellular, Molecular and Biomedical Reports, 2022 SARS-CoV- 2 is the causative agent of the global pandemic, also known as Covid-19. This virus belongs to a group of coronaviruses and has affected more than ten million people across the globe, causing nearly half a million deaths worldwide. The pandemic
Mohammad Mohammadi +2 more
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Stem Cell Reports, 2015 We present self-cloning CRISPR/Cas9 (scCRISPR), a technology that allows for CRISPR/Cas9-mediated genomic mutation and site-specific knockin transgene creation within several hours by circumventing the need to clone a site-specific single-guide RNA (sgRNA) or knockin homology construct for each target locus.
Mandana Arbab +5 more
openaire +9 more sources