Results 41 to 50 of about 277,742 (368)

Investigating the genomic background of CRISPR-Cas genomes for CRISPR-based antimicrobials [PDF]

arXiv, 2022
CRISPR-Cas systems are an adaptive immunity that protects prokaryotes against foreign genetic elements. Genetic templates acquired during past infection events enable DNA-interacting enzymes to recognize foreign DNA for destruction. Due to the programmability and specificity of these genetic templates, CRISPR-Cas systems are potential alternative ...
arxiv  

CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

Science, 2007
Clustered regularly interspaced short palindromic repeats (CRISPR) are a distinctive feature of the genomes of most Bacteria and Archaea and are thought to be involved in resistance to bacteriophages.
R. Barrangou, C. Fremaux, H. Deveau, Melissa Richards, P. Boyaval, S. Moineau, D. Romero, P. Horvath   +7 more
semanticscholar   +1 more source

CRISPR-Cas9 In Vivo Gene Editing for Transthyretin Amyloidosis.

New England Journal of Medicine, 2021
BACKGROUND Transthyretin amyloidosis, also called ATTR amyloidosis, is a life-threatening disease characterized by progressive accumulation of misfolded transthyretin (TTR) protein in tissues, predominantly the nerves and heart.
J. Gillmore, E. Gane, J. Taubel, J. Kao, M. Fontana, M. Maitland, Jessica Seitzer, D. O’Connell, Kathryn R Walsh, Kristy Wood, Jonathan Phillips, Yuanxin Xu, Adam Amaral, A. Boyd, J. Cehelsky, M. McKee, A. Schiermeier, O. Harari, A. Murphy, C. Kyratsous, B. Zambrowicz, Randy Soltys, D. Gutstein, J. Leonard, L. Sepp-Lorenzino, D. Lebwohl   +25 more
semanticscholar   +1 more source

Current applications and future perspective of CRISPR/Cas9 gene editing in cancer

Molecular Cancer, 2022
Clustered regularly interspaced short palindromic repeats (CRISPR) system provides adaptive immunity against plasmids and phages in prokaryotes. This system inspires the development of a powerful genome engineering tool, the CRISPR/CRISPR-associated ...
Si-wei Wang, Chao Gao, Yi-Min Zheng, Li Yi, Jia-Cheng Lu, Xiao-yong Huang, Jia-bin Cai, Peng-fei Zhang, Yuexin Cui, A. Ke   +9 more
semanticscholar   +1 more source

Putting the Spotlight on CRISPR [PDF]

BioTechniques, 2019
Rounding up the results from our recent spotlight on CRISPR – are we still just at the beginning for our CRISPR journey?
openaire   +6 more sources

Anti‐CRISPRs: The natural inhibitors for CRISPR‐Cas systems [PDF]

Animal Models and Experimental Medicine, 2019
AbstractCRISPR (clustered regularly interspaced short palindromic repeats)‐Cas (CRISPR associated protein) systems serve as the adaptive immune system by which prokaryotes defend themselves against phages. It has also been developed into a series of powerful gene‐editing tools. As the natural inhibitors of CRISPR‐Cas systems, anti‐CRISPRs (Acrs) can be
Fei Zhang, Guoxu Song, Yong Tian
openaire   +4 more sources

The precise magic of CRISPR [PDF]

FEBS Open Bio, 2021
In this issue of FEBS Open Bio, Shen Li et al., in the laboratory of Hector L. Franco (University of North Carolina), provide a proof‐of‐principle solution for correcting all copies of a gene in the widely used MCF7 breast cancer cell line. The gene for the FOXA1 pioneer transcription factor is localised on chromosome 14, which is present at least 4–5 ...
openaire   +3 more sources

The canonical single-stranded DNA-binding protein is not an essential replication factor but an RNA chaperon in Saccharolobus islandicus

iScience, 2023
Summary: Single-stranded DNA-binding proteins (SSBs) have been regarded as indispensable replication factors. Herein, we report that the genes encoding the canonical SSB (SisSSB) and the non-canonical SSB (SisDBP) in Saccharolobus islandicus REY15A are ...
Yuanxi Xiao, Zhichao Jiang, Mengqi Zhang, Xuemei Zhang, Qi Gan, Yunfeng Yang, Pengju Wu, Xu Feng, Jinfeng Ni, Xiuzhu Dong, Qunxin She, Qihong Huang, Yulong Shen   +12 more
doaj  

CRISPR-engineered T cells in patients with refractory cancer

Science, 2020
CRISPR takes first steps in humans CRISPR-Cas9 is a revolutionary gene-editing technology that offers the potential to treat diseases such as cancer, but the effects of CRISPR in patients are currently unknown. Stadtmauer et al. report a phase 1 clinical
E. Stadtmauer, J. Fraietta, M. Davis, A. Cohen, Kristy Weber, E. Lancaster, P. Mangan, I. Kulikovskaya, Minnal Gupta, Fang Chen, Lifeng Tian, V. Gonzalez, Jun Xu, In-Young Jung, J. Melenhorst, G. Plesa, J. Shea, Tina Matlawski, A. Cervini, A. Gaymon, Stephanie Desjardins, Anne Lamontagne, January Salas-McKee, A. Fesnak, D. Siegel, B. Levine, J. Jadlowsky, R. Young, A. Chew, W. Hwang, E. Hexner, B. Carreno, Christopher L. Nobles, F. Bushman, Kevin R. Parker, Yanyan Qi, Ansuman T. Satpathy, Howard Y. Chang, Yangbing Zhao, S. Lacey, C. June   +40 more
semanticscholar   +1 more source

Exponential family measurement error models for single-cell CRISPR screens [PDF]

arXiv, 2022
CRISPR genome engineering and single-cell RNA sequencing have accelerated biological discovery. Single-cell CRISPR screens unite these two technologies, linking genetic perturbations in individual cells to changes in gene expression and illuminating regulatory networks underlying diseases.
arxiv