Results 41 to 50 of about 291,035 (304)

SpCas9-expression by tumor cells can cause T cell-dependent tumor rejection in immunocompetent mice

OncoImmunology, 2019
The CRISPR/Cas9 system has recently emerged as a highly efficient modality in genetic engineering and has been widely considered for various therapeutic applications. However, since the effector protein, SpCas9, has a bacterial origin, its immunogenicity
Reham Ajina, Danielle Zamalin, Annie Zuo, Maha Moussa, Marta Catalfamo, Sandra A. Jablonski, Louis M. Weiner   +6 more
doaj   +1 more source

CRISPR-based assay reveals SARS-CoV-2 RNA dynamic changes and redistribution patterns in non-human primate model

Emerging Microbes and Infections, 2022
Mounting evidence indicates that SARS-CoV-2 can infect multiple systemic tissues, but few studies have evaluated SARS-CoV-2 RNA dynamics in multiple specimen types due to their reduced accessibility and diminished performance of RT-qPCR with non ...
Zhen Huang, Lili Zhang, Christopher J. Lyon, Bo Ning, Brady M. Youngquist, Alex Niu, Brandon J. Beddingfield, Nicholas J. Maness, Nakhle S. Saba, Chen-Zhong Li, Chad J. Roy, Tony Y. Hu   +11 more
doaj   +1 more source

CDK11 inhibition induces cytoplasmic p21WAF1 splice variant by p53 stabilisation and SF3B1 inactivation

Molecular Oncology, EarlyView.
CDK11 inhibition stabilises the tumour suppressor p53 and triggers the production of an alternative p21WAF1 splice variant p21L, through the inactivation of the spliceosomal protein SF3B1. Unlike the canonical p21WAF1 protein, p21L is localised in the cytoplasm and has reduced cell cycle‐blocking activity.
Radovan Krejcir, Lukasz Arcimowicz, Lucia Martinkova, Vaclav Hrabal, Filip Zavadil Kokas, Tomas Henek, Martina Kucerikova, Ondrej Bonczek, Pavlina Zatloukalova, Lenka Hernychova, Philip J. Coates, Borivoj Vojtesek, David P. Lane   +12 more
wiley   +1 more source

Current applications and future perspective of CRISPR/Cas9 gene editing in cancer

Molecular Cancer, 2022
Clustered regularly interspaced short palindromic repeats (CRISPR) system provides adaptive immunity against plasmids and phages in prokaryotes. This system inspires the development of a powerful genome engineering tool, the CRISPR/CRISPR-associated ...
Si-wei Wang, Chao Gao, Yi-Min Zheng, Li Yi, Jia-Cheng Lu, Xiao-yong Huang, Jia-Bin Cai, Peng-fei Zhang, Yuexin Cui, A. Ke   +9 more
semanticscholar   +1 more source

Identification of novel components of the Ced and Ups systems in Saccharolobus islandicus REY15A

mLife
In Sulfolobales cells, transcription of the Ups (UV‐inducible pili of Sulfolobus) and Ced (Crenarchaeal system for exchange of DNA) genes is highly induced by DNA damage, and the two systems play key roles in pili‐mediated cell aggregation and ...
Pengju Wu, Mengqi Zhang, Yanlu Kou, Shikuan Liang, Jinfeng Ni, Qihong Huang, Yulong Shen   +6 more
doaj   +1 more source

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

An Lrs14 family protein functions as a nucleoid-associated protein regulating cell cycle progression in Sulfolobales

Communications Biology
Archaea of the order Sulfolobales possess eukaryotic-like cell cycle. The chromatin organization in these archaea is supposed to rely on nucleoid-associated proteins (NAPs).
Qi Gan, Haodun Li, Qihong Huang, Yunfeng Yang, Fei Sun, Xu Feng, Jinfeng Ni, Zhenfeng Zhang, Qunxin She, Yulong Shen   +9 more
doaj   +1 more source

Cis‐regulatory and long noncoding RNA alterations in breast cancer – current insights, biomarker utility, and the critical need for functional validation

Molecular Oncology, EarlyView.
The noncoding region of the genome plays a key role in regulating gene expression, and mutations within these regions are capable of altering it. Researchers have identified multiple functional noncoding mutations associated with increased cancer risk in the genome of breast cancer patients.
Arnau Cuy Saqués, Aracele Martinez‐Mendez, John Crown, Alex Eustace   +3 more
wiley   +1 more source

MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens

Genome Biology, 2014
We propose the Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK) method for prioritizing single-guide RNAs, genes and pathways in genome-scale CRISPR/Cas9 knockout screens.
Wei Li, Han Xu, T. Xiao, Le Cong, Le Cong, M. Love, Feng Zhang, Feng Zhang, R. Irizarry, Jun S. Liu, Myles Brown, Myles Brown, X. Liu   +12 more
semanticscholar   +1 more source

Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for Saccharolobus islandicus

mLife
Saccharolobus islandicus REY15A represents one of the very few archaeal models with versatile genetic tools, which include efficient genome editing, gene silencing, and robust protein expression systems.
Pengpeng Zhao, Xiaonan Bi, Xiaoning Wang, Xu Feng, Yulong Shen, Guanhua Yuan, Qunxin She   +6 more
doaj   +1 more source