Results 41 to 50 of about 349,258 (340)
Production and analysis of synthetic Cascade variants [PDF]
, 2020 CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR assoziiert) ist ein adaptives Immunsystem in Archaeen und Bakterien, das fremdes genetisches Material mit Hilfe von Ribonukleoprotein-Komplexen erkennt und zerstört.
Gleditzsch, Daniel
core +1 more source
Engineering chromosome rearrangements in cancer
Disease Models & Mechanisms, 2021 The identification of large chromosomal rearrangements in cancers has multiplied exponentially over the last decade. These complex and often rare genomic events have traditionally been challenging to study, in part owing to lack of tools that efficiently
Salvador Alonso, Lukas E. Dow
doaj +1 more source
How the other half lives: CRISPR-Cas's influence on bacteriophages
, 2017 CRISPR-Cas is a genetic adaptive immune system unique to prokaryotic cells used to combat phage and plasmid threats. The host cell adapts by incorporating DNA sequences from invading phages or plasmids into its CRISPR locus as spacers.
AE Briner +35 more
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Current Protocols in Stem Cell Biology, 2016 AbstractCRISPR/Cas9‐gene editing has emerged as a revolutionary technology to easily modify specific genomic loci by designing complementary sgRNA sequences and introducing these into cells along with Cas9. Self‐cloning CRISPR/Cas9 (scCRISPR) uses a self‐cleaving palindromic sgRNA plasmid (sgPal) that recombines with short PCR‐amplified site‐specific ...
Mandana Arbab +2 more
openaire +3 more sources
Molecules, 2019 In CRISPR genome editing, CRISPR proteins form ribonucleoprotein complexes with guide RNAs to bind and cleave the target DNAs with complete sequence complementarity. CRISPR genome editing has a high potential for use in precision gene therapy for various
Taegeun Bae +6 more
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Putting the Spotlight on CRISPR [PDF]
BioTechniques, 2019 Rounding up the results from our recent spotlight on CRISPR – are we still just at the beginning for our CRISPR journey?
openaire +6 more sources
CRISPR-C: circularization of genes and chromosome by CRISPR in human cells [PDF]
Nucleic Acids Research, 2018 Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders. However, there is a lack of genetic engineering tools to recapitulate and study the biogenesis of eccDNAs. Here, we created a dual-fluorescence biosensor cassette, which upon the delivery of pairs of CRISPR/Cas9 guide RNAs, CRISPR-
Yonglun Luo +17 more
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BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]
, 2016 Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien +7 more
core +2 more sources
Archives of disease in childhood - Education & practice edition, 2016 Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology causing a major upheaval in biomedical research. It makes it possible to correct errors in the genome and turn on or off genes in cells and organisms quickly, cheaply and with relative ease.
Andrew J. King +3 more
openaire +2 more sources
A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila
Fly, 2020 Genome editing via homology-directed repair (HDR) has made possible precise and deliberate modifications to gene sequences. CRISPR/Cas9-mediated HDR is the simplest means to carry this out.
Kevin G. Nyberg +5 more
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