Results 41 to 50 of about 442,156 (406)

Current applications and future perspective of CRISPR/Cas9 gene editing in cancer

Molecular Cancer, 2022
Clustered regularly interspaced short palindromic repeats (CRISPR) system provides adaptive immunity against plasmids and phages in prokaryotes. This system inspires the development of a powerful genome engineering tool, the CRISPR/CRISPR-associated ...
Si-wei Wang, Chao Gao, Yi-Min Zheng, Li Yi, Jia-Cheng Lu, Xiao-yong Huang, Jia-bin Cai, Peng-fei Zhang, Yuexin Cui, A. Ke   +9 more
semanticscholar   +1 more source

A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila

Fly, 2020
Genome editing via homology-directed repair (HDR) has made possible precise and deliberate modifications to gene sequences. CRISPR/Cas9-mediated HDR is the simplest means to carry this out.
Kevin G. Nyberg, Joseph Q. Nguyen, Yong-Jae Kwon, Shelby Blythe, Greg J. Beitel, Richard Carthew   +5 more
doaj   +1 more source

Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi [PDF]

, 2019
Background: Within the last years, numerous reports described successful application of the CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi.
Haefner, Stefan, Kwon, Jin, Meyer, Vera, Schütze, Tabea, Spohner, Sebastian   +4 more
core   +1 more source

The influence of the copy number of invader on the fate of bacterial host cells in the antiviral defense by CRISPR-Cas10 DNases

Engineering Microbiology, 2023
Type III CRISPR-Cas10 systems employ multiple immune activities to defend their hosts against invasion from mobile genetic elements (MGEs), including DNase and cyclic oligoadenylates (cOA) synthesis both of which are hosted by the type-specific protein ...
Zhenxiao Yu, Jianan Xu, Yan Zhang, Qunxin She   +3 more
doaj   +1 more source

A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

National Science Review, 2022
The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed.
Zhi Chen, Jingfeng Li, Tianzhong Li, Taojian Fan, Changle Meng, Chao Li, Jianlong Kang, Luxiao Chai, Yabin Hao, Yuxuan Tang, O. Al-Hartomy, S. Wageh, Abdullah G. Al‐Sehemi, Zhiguang Luo, Jiangtian Yu, Y. Shao, Defa Li, Shuai Feng, William J. Liu, Yaqing He, Xiao-Ping Ma, Zhongjian Xie, Han Zhang   +22 more
semanticscholar   +1 more source

CRISPR-engineered T cells in patients with refractory cancer

Science, 2020
CRISPR takes first steps in humans CRISPR-Cas9 is a revolutionary gene-editing technology that offers the potential to treat diseases such as cancer, but the effects of CRISPR in patients are currently unknown. Stadtmauer et al. report a phase 1 clinical
E. Stadtmauer, J. Fraietta, M. Davis, A. Cohen, Kristy Weber, E. Lancaster, P. Mangan, I. Kulikovskaya, Minnal Gupta, Fang Chen, Lifeng Tian, V. Gonzalez, Jun Xu, In-Young Jung, J. Melenhorst, G. Plesa, J. Shea, Tina Matlawski, A. Cervini, A. Gaymon, Stephanie Desjardins, Anne Lamontagne, January Salas-McKee, A. Fesnak, D. Siegel, B. Levine, J. Jadlowsky, R. Young, A. Chew, W. Hwang, E. Hexner, B. Carreno, Christopher L. Nobles, F. Bushman, Kevin R. Parker, Yanyan Qi, Ansuman T. Satpathy, Howard Y. Chang, Yangbing Zhao, S. Lacey, C. June   +40 more
semanticscholar   +1 more source

Anti‐CRISPRs: The natural inhibitors for CRISPR‐Cas systems [PDF]

Animal Models and Experimental Medicine, 2019
AbstractCRISPR (clustered regularly interspaced short palindromic repeats)‐Cas (CRISPR associated protein) systems serve as the adaptive immune system by which prokaryotes defend themselves against phages. It has also been developed into a series of powerful gene‐editing tools. As the natural inhibitors of CRISPR‐Cas systems, anti‐CRISPRs (Acrs) can be
Fei Zhang, Guoxu Song, Yong Tian
openaire   +4 more sources

Parkin-independent mitophagy controls chemotherapeutic response in cancer cells [PDF]

, 2017
Mitophagy is an evolutionarily conserved process that selectively targets impaired mitochondria for degradation. Defects in mitophagy are often associated with diverse pathologies, including cancer.
Bossowski, Jozef P., Chiche, Johanna, Marchetti, Sandrine, Mondragón, Laura, Obba, Sandrine, Proïcs, Emma, Ricci, Jean-Ehrland, Riley, Joel S., Rozier, Romain M., Rubio-Patiño, Camila, Tait, Stephen W.G., Verhoeyen, Els, Villa, Elodie, Zunino, Barbara   +13 more
core   +2 more sources

CRISPR-Cas9 In Vivo Gene Editing for Transthyretin Amyloidosis.

New England Journal of Medicine, 2021
BACKGROUND Transthyretin amyloidosis, also called ATTR amyloidosis, is a life-threatening disease characterized by progressive accumulation of misfolded transthyretin (TTR) protein in tissues, predominantly the nerves and heart.
J. Gillmore, E. Gane, J. Taubel, J. Kao, M. Fontana, M. Maitland, Jessica Seitzer, D. O’Connell, Kathryn R Walsh, Kristy Wood, Jonathan Phillips, Yuanxin Xu, Adam Amaral, A. Boyd, J. Cehelsky, M. McKee, A. Schiermeier, O. Harari, A. Murphy, C. Kyratsous, B. Zambrowicz, Randy Soltys, D. Gutstein, J. Leonard, L. Sepp-Lorenzino, D. Lebwohl   +25 more
semanticscholar   +1 more source

Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery [PDF]

, 2018
Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein-9 (CRISPR-Cas9) can be used as an efficient tool for genome editing in potato (Solanum tuberosum).
Andersson, Andersson, Cho, Clasen, Eriksson, Gorbunova, Hartung, Jinek, Jones, Kim, Kuipers, Kuipers, Larkin, Liang, Muthoni, Nelson, Sawai, Shepard, Svitashev, Visser, Woo, Xu, Zhang   +22 more
core   +1 more source