Results 51 to 60 of about 277,742 (368)
Proofreading mechanism of Class 2 CRISPR-Cas systems [PDF]
, 2020 CRISPR systems experience off-target effects that interfere with the ability to accurately perform genetic edits. While empirical models predict off-target effects in specific platforms, there is a gap for a wide-ranging mechanistic model of CRISPR systems based on evidence from the essential processes of target recognition.
arxiv +1 more source
Current Protocols in Stem Cell Biology, 2016 AbstractCRISPR/Cas9‐gene editing has emerged as a revolutionary technology to easily modify specific genomic loci by designing complementary sgRNA sequences and introducing these into cells along with Cas9. Self‐cloning CRISPR/Cas9 (scCRISPR) uses a self‐cleaving palindromic sgRNA plasmid (sgPal) that recombines with short PCR‐amplified site‐specific ...
Mandana Arbab +2 more
openaire +3 more sources
Molecules, 2019 In CRISPR genome editing, CRISPR proteins form ribonucleoprotein complexes with guide RNAs to bind and cleave the target DNAs with complete sequence complementarity. CRISPR genome editing has a high potential for use in precision gene therapy for various
Taegeun Bae +6 more
doaj +1 more source
Archives of disease in childhood - Education & practice edition, 2016 Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology causing a major upheaval in biomedical research. It makes it possible to correct errors in the genome and turn on or off genes in cells and organisms quickly, cheaply and with relative ease.
Andrew J. King +3 more
openaire +2 more sources
Harnessing Nature’s Diversity for Developing New Biotechnologies [PDF]
Molecular Frontiers Journal, 2019 Molecular biologists today make use of a number of technologies based on natural products or systems, essentially using the wonders of life to further discover wonderous things about life. This cycle is powered by the immense amount of natural diversity.
Feng Zhang
doaj +1 more source
A CRISPR View of Cleavage [PDF]
Cell, 2015 Seminal studies showed that CRISPR-Cas systems provide adaptive immunity in prokaryotes and promising gene-editing tools from bacteria to humans. Yet, reports diverged on whether some CRISPR systems naturally target DNA or RNA. Here, Samai and colleagues unify the studies, showing that a single type III CRISPR-Cas system cleaves both DNA and RNA ...
Michael S. Gilmore +2 more
openaire +3 more sources
A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids [PDF]
Royal Society Open Science, 2017 Clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR-associated gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens, including kinetoplastids.
Tom Beneke +5 more
doaj +1 more source
Prokaryotic genome editing based on the subtype I-B-Svi CRISPR-Cas system [PDF]
arXiv, 2023 Type I CRISPR-Cas systems are the most common among six types of CRISPR-Cas systems, however, non-self-targeting genome editing based on a single Cas3 of type I CRISPR-Cas systems has not been reported. Here, we present the subtype I-B-Svi CRISPR-Cas system (with three confirmed CRISPRs and a cas gene cluster) and genome editing based on this system ...
arxiv
Virulence, 2021 Severe Helicobacter pylori-linked gastric disorders are especially prevalent in the East Asia region. The ability of H. pylori to cause different clinical outcomes is thought to be associated with unique sets of its genetic features.
Feng Yang +16 more
doaj +1 more source
Crispr strategies for stem cell engineering: a new frontier in musculoskeletal regeneration
European Cells & Materials, 2023 The costs and incidence of musculoskeletal injuries are rapidly increasing due to rising population age, higher prevalence of risk factors, and lack of effective long-term treatments.
JP Graham +3 more
doaj +1 more source