Results 51 to 60 of about 291,035 (304)

Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma

Molecular Oncology, EarlyView.
Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken, Andrea Abel Gutierrez, Imke van Rossum, Cornelia G. Spruijt, Michiel Vermeulen, Guido van Mierlo, Blanca Scheijen, Annemiek B. van Spriel   +7 more
wiley   +1 more source

Modulation of Hachiman defence by a type II toxin-antitoxin system via balancing trade-off between the fitness cost and antiphage activity

Engineering Microbiology
Hachiman systems provide innate antiphage immunity across prokaryotic domains. The system encodes a HamA nuclease and a HamB helicase both of which exhibit great diversity in sequence.
Xuhui Tian, Ruyi Zheng, Xin Li, Suping Jiang, Fang Wang, Yulong Shen, Qunxin She   +6 more
doaj   +1 more source

uPAR Knockout Results in a Deep Glycolytic and OXPHOS Reprogramming in Melanoma and Colon Carcinoma Cell Lines

Cells, 2020
Urokinase Plasminogen Activator (uPA) Receptor (uPAR) is a well-known GPI-anchored three-domain membrane protein with pro-tumor roles largely shown in all the malignant tumors where it is over-expressed.
Alessio Biagioni, Anna Laurenzana, Anastasia Chillà, Mario Del Rosso, Elena Andreucci, Martina Poteti, Daniele Bani, Daniele Guasti, Gabriella Fibbi, Francesca Margheri   +9 more
doaj   +1 more source

Plecstatin inhibits hepatocellular carcinoma tumorigenesis and invasion through cytolinker plectin

Molecular Oncology, EarlyView.
The ruthenium‐based metallodrug plecstatin exerts its anticancer effect in hepatocellular carcinoma (HCC) primarily through selective targeting of plectin. By disrupting plectin‐mediated cytoskeletal organization, plecstatin inhibits anchorage‐dependent growth, cell polarization, and tumor cell dissemination.
Zuzana Outla, Jan Kosla, Magdalena Prechova, Lukas Frick, Patricia Bortel, Yasmin Borutzki, Andrea Bileck, Christopher Gerner, Samuel M. Meier‐Menches, Selma Osmanagic‐Myers, Martin Gregor   +10 more
wiley   +1 more source

Past, present, and future of CRISPR genome editing technologies.

Cell
Genome editing has been a transformative force in the life sciences and human medicine, offering unprecedented opportunities to dissect complex biological processes and treat the underlying causes of many genetic diseases. CRISPR-based technologies, with
Martin Pacesa, O. Pelea, M. Jinek
semanticscholar   +1 more source

A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

National Science Review, 2022
The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed.
Zhi Chen, Jingfeng Li, Tianzhong Li, Taojian Fan, Changle Meng, Chao Li, Jianlong Kang, Luxiao Chai, Yabin Hao, Yuxuan Tang, O. Al-Hartomy, S. Wageh, Abdullah G. Al‐Sehemi, Zhiguang Luo, Jiangtian Yu, Y. Shao, Defa Li, Shuai Feng, William J. Liu, Yaqing He, Xiao-Ping Ma, Zhongjian Xie, Han Zhang   +22 more
semanticscholar   +1 more source

Peroxidasin enables melanoma immune escape by inhibiting natural killer cell cytotoxicity

Molecular Oncology, EarlyView.
Peroxidasin (PXDN) is secreted by melanoma cells and binds the NK cell receptor NKG2D, thereby suppressing NK cell activation and cytotoxicity. PXDN depletion restores NKG2D signaling and enables effective NK cell–mediated melanoma killing. These findings identify PXDN as a previously unrecognized immune evasion factor and a potential target to improve
Hsu‐Min Sung, David Bickel, Lena C. M. Krause, Daria Ezeriņa, Christian Ickes, Julian Wojtachnia, Christine S. Gibhardt, Magdalena Shumanska, Khadija Wahni, Andrea Paluschkiwitz, Julia Malo Pueyo, Ekaterina Baranova, Wim Vranken, Hedwig Stanisz, Ioana Stejerean‐Todoran, Michael P. Schön, Joris Messens, Ivan Bogeski   +17 more
wiley   +1 more source

CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens

Nucleic Acids Res., 2018
CRISPOR.org is a web tool for genome editing experiments with the CRISPR–Cas9 system. It finds guide RNAs in an input sequence and ranks them according to different scores that evaluate potential off-targets in the genome of interest and predict on ...
J. Concordet, M. Haeussler
semanticscholar   +1 more source

Flexible TAM requirement of TnpB enables efficient single-nucleotide editing with expanded targeting scope

Nature Communications
TnpBs encoded by the IS200/IS605 family transposon are among the most abundant prokaryotic proteins from which type V CRISPR-Cas nucleases may have evolved.
Xu Feng, Ruyi Xu, Jianglan Liao, Jingyu Zhao, Baochang Zhang, Xiaoxiao Xu, Pengpeng Zhao, Xiaoning Wang, Jianyun Yao, Pengxia Wang, Xiaoxue Wang, Wenyuan Han, Qunxin She   +12 more
doaj   +1 more source

CRISPR patent results [PDF]

Nature Reviews Microbiology, 2017
The CRISPR–Cas9 patents filed by the Broad Institute are upheld by the US Patent and Trademark ...
openaire   +2 more sources