Results 51 to 60 of about 321,595 (358)

MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens

Genome Biology, 2014
We propose the Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK) method for prioritizing single-guide RNAs, genes and pathways in genome-scale CRISPR/Cas9 knockout screens.
Wei Li, Han Xu, T. Xiao, Le Cong, Le Cong, M. Love, Feng Zhang, Feng Zhang, R. Irizarry, Jun S. Liu, Myles Brown, Myles Brown, X. Liu   +12 more
semanticscholar   +1 more source

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

Science, 2020
A PAMless base editor CRISPR-Cas DNA base editing typically requires a specific motif for targeting known as a protospacer-adjacent motif (PAM). This requirement limits the sequences within a genome that can be targeted. Walton et al. engineered specific
R. Walton, K. Christie, Madelynn N. Whittaker, B. Kleinstiver   +3 more
semanticscholar   +1 more source

Predictors of response and rational combinations for the novel MCL‐1 inhibitor MIK665 in acute myeloid leukemia

Molecular Oncology, EarlyView.
This study characterizes the responses of primary acute myeloid leukemia (AML) patient samples to the MCL‐1 inhibitor MIK665. The results revealed that monocytic differentiation is associated with MIK665 sensitivity. Conversely, elevated ABCB1 expression is a potential biomarker of resistance to the treatment, which can be overcome by the combination ...
Joseph Saad, Rhiannon Newman, Elmira Khabusheva, Sofia Aakko, Eric Durand, Mahesh Tambe, Heikki Kuusanmäki, Alun Parsons, Juho J. Miettinen, Komal Kumar Javarappa, Ezgi June Olgac, Nemo Ikonen, Mika Kontro, Kimmo Porkka, Heiko Maacke, Janghee Woo, Ensar Halilovic, Caroline A. Heckman   +17 more
wiley   +1 more source

Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for Saccharolobus islandicus

mLife
Saccharolobus islandicus REY15A represents one of the very few archaeal models with versatile genetic tools, which include efficient genome editing, gene silencing, and robust protein expression systems.
Pengpeng Zhao, Xiaonan Bi, Xiaoning Wang, Xu Feng, Yulong Shen, Guanhua Yuan, Qunxin She   +6 more
doaj   +1 more source

GuideMaker: Software to design CRISPR-Cas guide RNA pools in non-model genomes [PDF]

, 2022
Ravin Poudel, Lidimarie Trujillo Rodríguez, Christopher R. Reisch, Adam R. Rivers   +3 more
openalex   +1 more source

Computational correction of copy-number effect improves specificity of CRISPR-Cas9 essentiality screens in cancer cells

Nature Genetics, 2017
The CRISPR–Cas9 system has revolutionized gene editing both at single genes and in multiplexed loss-of-function screens, thus enabling precise genome-scale identification of genes essential for proliferation and survival of cancer cells.
R. Meyers, Jordan G. Bryan, James M. McFarland, B. Weir, Ann E. Sizemore, Han Xu, N. Dharia, Phillip G. Montgomery, G. Cowley, S. Pantel, A. Goodale, Yenarae Lee, L. Ali, G. Jiang, Rakela Lubonja, W. Harrington, M. Strickland, Ting Wu, Derek Hawes, Victor A. Zhivich, M. Wyatt, Zohra Kalani, Jaime Chang, M. Okamoto, T. Golub, J. Boehm, F. Vazquez, D. Root, W. Hahn, Aviad Tsherniak   +29 more
semanticscholar   +1 more source

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source

Modulation of Hachiman defence by a type II toxin-antitoxin system via balancing trade-off between the fitness cost and antiphage activity

Engineering Microbiology
Hachiman systems provide innate antiphage immunity across prokaryotic domains. The system encodes a HamA nuclease and a HamB helicase both of which exhibit great diversity in sequence.
Xuhui Tian, Ruyi Zheng, Xin Li, Suping Jiang, Fang Wang, Yulong Shen, Qunxin She   +6 more
doaj   +1 more source

uPAR Knockout Results in a Deep Glycolytic and OXPHOS Reprogramming in Melanoma and Colon Carcinoma Cell Lines

Cells, 2020
Urokinase Plasminogen Activator (uPA) Receptor (uPAR) is a well-known GPI-anchored three-domain membrane protein with pro-tumor roles largely shown in all the malignant tumors where it is over-expressed.
Alessio Biagioni, Anna Laurenzana, Anastasia Chillà, Mario Del Rosso, Elena Andreucci, Martina Poteti, Daniele Bani, Daniele Guasti, Gabriella Fibbi, Francesca Margheri   +9 more
doaj   +1 more source

Comprehensive review of CRISPR-based gene editing: mechanisms, challenges, and applications in cancer therapy

Molecular Cancer
The CRISPR system is a revolutionary genome editing tool that has the potential to revolutionize the field of cancer research and therapy. The ability to precisely target and edit specific genetic mutations that drive the growth and spread of tumors has ...
Mohammad Chehelgerdi, Matin Chehelgerdi, Milad Khorramian-Ghahfarokhi, Marjan Shafieizadeh, E. Mahmoudi, Fatemeh Eskandari, Mohsen Rashidi, A. Arshi, Abbas Mokhtari-Farsani   +8 more
semanticscholar   +1 more source