The application of a generative diffusion model, enhanced with a training data augmentation pipeline retaining the manufacturing process context of electrode microstructures, leads to improved fidelity of the through‐plane tortuosity factor in the AI generated samples.
Victor Ramirez‐Camacho +5 more
wiley +1 more source
Structural basis for T-cell intracellular antigen-1 amyloid fibril formation revealed by cryo-electron microscopy. [PDF]
Inaoka D +13 more
europepmc +1 more source
Cell Segmentation Beyond 2D—A Review of the State‐of‐the‐Art
Cell segmentation underpins many biological image analysis tasks, yet most deep learning methods remain limited to 2D despite the inherently 3D nature of cellular processes. This review surveys segmentation approaches beyond 2D, comparing 2.5D and fully 3D methods, analyzing 31 models and 32 volumetric datasets, and introducing a unified reference ...
Fabian Schmeisser +6 more
wiley +1 more source
Integrating in situ single-particle cryo-electron microscopy with cryo-electron tomography for high-resolution structural biology in native cellular contexts. [PDF]
Yue J, Guo W, Liu J.
europepmc +1 more source
This article investigates how persistent homology, persistent Laplacians, and persistent commutative algebra reveal complementary geometric, topological, and algebraic invariants or signatures of real‐world data. By analyzing shapes, synthetic complexes, fullerenes, and biomolecules, the article shows how these mathematical frameworks enhance ...
Yiming Ren, Guo‐Wei Wei
wiley +1 more source
Dose-efficient cryo-electron microscopy for thick samples using tilt- corrected scanning transmission electron microscopy. [PDF]
Yu Y +12 more
europepmc +1 more source
AI‐based tools enable rapid characterization of bacterial ultrastructure in low‐dose cryogenic transmission electron microscopy. The envelope thickness tool quantifies membrane thickness and anisotropy. The flagella module analyzes filament morphology and detects cell‐flagella contacts.
Sita Sirisha Madugula +10 more
wiley +1 more source
Constitutively active orphan G protein-coupled receptors through the lenses of cryo-electron microscopy. [PDF]
Argerich J +3 more
europepmc +1 more source
Click'n ROPISA: One‐Pot Synthesis of Ultrabright Fluorescent Nanoparticles Made of Polypeptides
Self‐assembly and fluorophore conjugation proceed rapidly in a one‐pot “Click'n ROPISA” process, yielding intensely fluorescent polypeptide nanoparticles with tunable visible emission. High dye loading enables efficient energy transfer and single‐particle visualization, while the straightforward synthesis provides versatile access to bright ...
Amin El Jarroudi Hammou +7 more
wiley +1 more source
Reply to Comment on ‘Electromagnetic lensing using the Aharonov–Bohm effect’
Makoto Tokoro Schreiber, Matthias Wolf
doaj +1 more source

