Results 221 to 230 of about 2,310,044 (293)

Measurement of atomic scattering factors by cryo-electron microscopy

Shtyrov A, Wilson H, Slowik D, Yamashita K, Li J, Wojdyr M, Chen S, McMullan G, Short J, Russo CJ, Henderson R, Murshudov GN.   +11 more
europepmc   +1 more source

Purification of the Active-State G Protein-Coupled Receptor ADGRL4 for Cryo-Electron Microscopy Using a Modular Tag System and a Tethered mini-G_q. [PDF]

Bio Protoc
Favara DM, Tate CG.
europepmc   +1 more source

Modifications of Lysine Residues 294 and 311 Within the Microtubule‐Binding Region Alter Tau Aggregation and Tubulin Binding Equilibria

Angewandte Chemie, EarlyView.
The molecular consequences of specific lysine modifications in Alzheimer´s disease remain insufficiently resolved in the context of full‐length protein. Here we integrate protein semisynthesis, segmental isotope labelling, and high‐resolution NMR spectroscopy to achieve residue‐resolved interrogation of site‐specific acetylation and carboxymethylation.
Dominik P. Vogl, Mario Migotti, Robert Konrat, Anne C. Conibear, Christian F. W. Becker   +4 more
wiley   +1 more source

Native state structural and chemical characterisation of Pickering emulsions: A cryo-electron microscopy study. [PDF]

J Microsc
Fernandez Ainaga DL, Roncal-Herrero T, Ilett M, Aslam Z, Cheng C, Hitchcock JP, Cayre OJ, Hondow N.   +7 more
europepmc   +1 more source

Characterization of Full‐Length Antibody Complexes Through HDX‐Mass Spectrometry Coupled With NMR Spectroscopy: New Insight Into Avelumab/PD‐L1 Interaction

Angewandte Chemie, EarlyView.
Combining HDX‐MS and NMR enables the mapping of epitopes and paratopes in full‐length antibody complexes, revealing transient interactions and potential artifacts. ABSTRACT Recombinant biomacromolecules have significantly transformed pharmaceuticals, in particular monoclonal antibodies (mAbs) that target key areas in oncology, immunology, inflammation,
Francesca Sacco, Fabio Baroni, Angela Capolupo, Sofia Petrocchi, Jonathan Zöller, Linda Cerofolini, Pasquale Russomanno, Rebecca Calamandrei, Bianca Susini, Siyu Lin, Fabio D'Amici, Enrico Ravera, Julian Langer, Marco Fragai   +13 more
wiley   +1 more source

Beyond Canonical CO Oxidation: Structural and Evolutionary Insights Into a Non‐Canonical Carbon Monoxide Dehydrogenase

Angewandte Chemie, EarlyView.
Clade B carbon monoxide dehydrogenases (CODHs) remain enigmatic, diverging from canonical CODHs in structure and function. Here, we present the first characterization of Ruminococcus flavefaciens CODH (RfCODH), revealing occluded substrate pathways and attenuated CO oxidation activity.
Maximilian Böhm, Vivek Srinivas, Benjamin Wiseman, Ping Huang, Moritz Senger, Martin Högbom, Henrik Land   +6 more
wiley   +1 more source

Decoys Reveal Multiple Basins of Attraction for Cryo-Electron-Microscopy Flexible Fitting. [PDF]

Comput Struct Bioinform (2024)
Alshammari M, He J, Wriggers W.
europepmc   +1 more source

Reusable Polyphenol Building Blocks Enable Recyclable Epoxy‐Amine Thermosets via Methanolysis

Angewandte Chemie, EarlyView.
A biobased epoxy–amine platform was developed using functional building blocks derived from polyphenols. These thermosets exhibited tunable mechanical and thermal properties, together with efficient, catalyst‐free chemical recycling via methanolysis. Beyond the recovery of reusable building blocks, the recycled polyols could be further upcycled into ...
Yi‐Min Tu, Yuhang Jiang, Kun Li, Qing Cao, Yizhe Pan, Christoph Tzschucke, Andreas J. Achazi, Rainer Haag   +7 more
wiley   +1 more source

Distinct Molten‐Globule Conformations in an Interacting Protein Domain Resolved by 19F NMR of Fluoroproline Residues

Angewandte Chemie, EarlyView.
Modifying the conformational ensemble of “molten‐globule” protein by the site‐specific incorporation of stereoisomeric 4‐fluoroproline residues modulated its binding affinity and aggregation behavior. 19F NMR spectroscopy enabled the detection of key interactions responsible for structural and dynamic changes. ABSTRACT Recently, the application of deep
Abir Ben Bouzayene, Mariam Sai, Alexis Jouin, Yves Nominé, Vladimir Torbeev, Bruno Kieffer   +5 more
wiley   +1 more source

Simultaneous polyclonal antibody sequencing and epitope mapping by cryo electron microscopy and mass spectrometry. [PDF]

Elife
Schulte D, Šiborová M, Käll L, Snijder J.   +3 more
europepmc   +1 more source