Coconut Cryopreservation: Present Status and Future Prospects
Coconut is one of the most important small holder crops worldwide. Conservation of coconut as seeds or field gene banks is not effective due to a range of limitations.
Iroshini Welewanni, Dharshani Bandupriya
doaj +1 more source
Cryopreservation of sugarcane embryogenic callus using a simplified freezing process [PDF]
A simplified freezing process was developed and successfully applied to embryogenic calluses of three sugarcane commercial hybrids (Saccharum sp. cv. CP 5243, C 91-301 and C 1051-73). To obtain optimal survival, the calluses were pretreated with a cryoprotective solution containing 10% DMSO and 0.3 to 0.75M sucrose.
arxiv
Calculation of a temperature-volume phase diagram of water to inform the study of isochoric freezing down to cryogenic temperatures [PDF]
Phase diagrams are integral to the application and interpretation of materials thermodynamics, and none is more ubiquitous than the common Temperature-Pressure diagram of water and its many icy phases. Inspired by recent advances in isochoric thermodynamics, we here employ a simple convex hull approach to efficiently calculate an updated Temperature ...
arxiv
Apoptotic Cell Death and Function of Cryopreserved Porcine Hepatocytes in a Bioartificial Liver
We have previously shown that cryopreservation leads to increased apoptotic death of porcine hepatocytes intended for use in a bioartificial liver (BAL).
Takakazu Matsushita+7 more
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Cryopreservation of pineapple (Ananas comosus) apices [PDF]
The encapsulation-dehydration and vitrification techniques were experimented for freezing apices of pineapple in vitro plantlets. Positive results were achieved using vitrification only. Optimal conditions included a 2-d preculture of apices on medium supplemented with 0.3M sucrose, loading treatment for 25 min in medium with 0.75M sucrose + 1M ...
arxiv
Premelting-directed interfacial design of nanoshell-coated cells in directional freezing and thawing [PDF]
Freezing interface interacting with soft cells is a core issue in cryopreservation. However, most of existing cryopreservation approaches face challenges such as complex processing and poor controllability. Herein we report a new, nanotechnology-based method to modulate interfacial interactions between ice and cells via the premelting theory.
arxiv
Effects of cryoprotectant concentration and cooling rate on vitrification of aqueous solutions [PDF]
Vitrification of aqueous cryoprotectant mixtures is essential in cryopreservation of proteins and other biological samples. We report systematic measurements of critical cryoprotective agent (CPA) concentrations required for vitrification during plunge cooling from T=295 K to T=77 K in liquid nitrogen.
arxiv +1 more source
Background: Luteinizing hormone (LH) is essential for normal follicular development and oocyte maturation. In particular, fluctuations of LH during the follicular phase have a significant impact on morphological and functional changes of the oocyte and ...
Raffaella Depalo+5 more
doaj
Cryopreservation Techniques for Ram Sperm
Germplasm storage and transportation in artificial insemination (AI) and other advanced technologies are facilitated by cryopreservation. In reproduction, the cryopreservation of sperm allows it to be transported across vast distances and used even after
Amit Saha+2 more
doaj +1 more source
Quantum superposition, entanglement, and state teleportation of a microorganism on an electromechanical oscillator [PDF]
Schr\"odinger's thought experiment to prepare a cat in a superposition of both alive and dead states reveals profound consequences of quantum mechanics and has attracted enormous interests. Here we propose a straightforward method to create quantum superposition states of a living microorganism by putting a small cryopreserved bacterium on top of an ...
arxiv +1 more source