Results 71 to 80 of about 118,025 (366)

Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors [PDF]

, 2017
Human biliary tree stem/progenitor cells (hBTSCs) are being used for cell therapies of patients with liver cirrhosis. A cryopreservation method was established to optimize sourcing of hBTSCs for these clinical programs and that comprises serum-free ...
Aliberti, Camilla, Alvaro, Domenico, Bastianelli, Carlo, Berloco, Pasquale Bartolomeo, Bosco, Daniela, Brunelli, Roberto, Cantafora, Alfredo, Cardinale, Vincenzo, Carpino, Guido, Costantini, Daniele, DI MATTEO, Sabina, Gaudio, Eugenio, Melandro, Fabio, Monti, Marco, Nevi, Lorenzo, Panici, Pierluigi Benedetti, Reid, Lola   +16 more
core   +2 more sources

Clinical Impact of Graft Cryopreservation on Allogeneic Stem Cell Transplantation: An Italian, Registry‐Based Study on Behalf of the “Gruppo Italiano Per Il Trapianto di Midollo Osseo, Cellule Staminali Emopoietiche e Terapia Cellulare” (GITMO)

American Journal of Hematology, EarlyView.
ABSTRACT The coronavirus disease 2019 (COVID‐19) pandemic created major challenges for allogeneic hematopoietic stem cell transplantation (allo‐HSCT). Scientific societies and authorities recommended cryopreserving grafts before starting conditioning regimens, despite limited data on the clinical impact.
Irene Defrancesco, Virginia Valeria Ferretti, Patrizia Chiusolo, Domenico Russo, Chiara Nozzoli, Attilio Olivieri, Massimiliano Gambella, Irene Maria Cavattoni, Stefania Bramanti, Stella Santarone, Renato Fanin, Roberto Cairoli, Simona Piemontese, Matteo Parma, Francesco Onida, Alessandro Busca, Luca Castagna, Angela Cuoghi, Domenico Pastore, Nicola Mordini, Fabio Benedetti, Cristina Skert, Carlo Borghero, Anna Paola Iori, Franca Fagioli, Vincenzo Pavone, Carmine Selleri, Simone Cesaro, Maurizio Musso, Marco Ladetto, Daniele Vallisa, Paola Carluccio, Alessandra Picardi, Monica Tozzi, Alessandra Biffi, Giuseppe Milone, Maura Faraci, Arcangelo Prete, Lucia Prezioso, Antonio Maria Risitano, Francesco Paolo Tambaro, Veronica Tintori, Piero Galieni, Fabrizio Pane, Caterina Zerbi, Antonio Bianchessi, Giulia Losi, Francesco Romano, Alessia Taurino, Elena Oldani, Nicola Polverelli, Francesca Bonifazi, Massimo Martino   +52 more
wiley   +1 more source

Cryopreservation of plant germplasm in Argentina [PDF]

, 2007
This review describes the current status of development of methods for cryopreservation (at -196ºC) of plants germplasm in Argentina. Arachis pintoi, a forage legume, has been maintained as seeds using vitrification method. Additionally, apical meristems,
Mroginski, Luis Amado, Rey de Badaró, Hebe Yolanda   +1 more
core  

A Mathematical Model for Determining Probabilistic Design Space in Mesenchymal Stem Cell Passage Culture

Biotechnology and Bioengineering, EarlyView.
ABSTRACT With their many therapeutic functions, mesenchymal stem cells (MSCs) are promising sources for regenerative medicine. However, in the manufacture of MSCs, without a method for exploring the effects of long‐term passage on cell proliferation potentials, the design of passage culture processes is challenging.
Keita Hirono, Yusuke Hayashi, Yuuki Ogawa, Masahiro Kino‐oka, Hirokazu Sugiyama   +4 more
wiley   +1 more source

Apoptotic Cell Death and Function of Cryopreserved Porcine Hepatocytes in a Bioartificial Liver

Cell Transplantation, 2003
We have previously shown that cryopreservation leads to increased apoptotic death of porcine hepatocytes intended for use in a bioartificial liver (BAL).
Takakazu Matsushita, Toshikazu Yagi, Joseph A. Hardin, Jennifer D. Cragun, Frank W. Crow, H. Robert Bergen, Gregory J. Gores, Scott L. Nyberg M.D., Ph.D.   +7 more
doaj   +1 more source

Premelting-directed interfacial design of nanoshell-coated cells in directional freezing and thawing [PDF]

arXiv, 2020
Freezing interface interacting with soft cells is a core issue in cryopreservation. However, most of existing cryopreservation approaches face challenges such as complex processing and poor controllability. Herein we report a new, nanotechnology-based method to modulate interfacial interactions between ice and cells via the premelting theory.
arxiv  

Calculation of a temperature-volume phase diagram of water to inform the study of isochoric freezing down to cryogenic temperatures [PDF]

arXiv, 2021
Phase diagrams are integral to the application and interpretation of materials thermodynamics, and none is more ubiquitous than the common Temperature-Pressure diagram of water and its many icy phases. Inspired by recent advances in isochoric thermodynamics, we here employ a simple convex hull approach to efficiently calculate an updated Temperature ...
arxiv  

Optimizing biophysical properties of cellular niches to enhance stem cell‐derived extracellular vesicle function in musculoskeletal regeneration

BMEMat, EarlyView.
Extracellular vesicles (EVs) derived from stem cells show promise for applications in regenerative medicine, but their scalability and yield remain challenges. This review explores the approaches for biophysical modulations within cell niches on EV properties, discusses the current clinical application of EVs, and provides an outlook on the existing ...
Yang Xu, Chung Yin Matthew Cheung, Liling Liu, Hoi Pan Harry Cheung, Kam Yu Tam, Dai Fei Elmer Ker, Sarah H. Cartmell, Chuanbin Mao, Zhiyong Zhang, Dan Michelle Wang   +9 more
wiley   +1 more source

133 Investigating the Utilization Rate of Fertility Preservation Services Amongst Transgender and Gender Diverse Patients

Journal of Clinical and Translational Science, 2022
OBJECTIVES/GOALS: Fertility preservation (FP) allows transgender and gender diverse (TGD) patients undergoing gender-affirming therapy to pursue genetic parenthood. Barriers to care exist leading to its underutilization; while these barriers to care have
Karen DSouza, Yeon Soo Lee, Dzhuliyan J. Vasilev, Megan Allyse, Alessandra Ainsworth, Caroline J. Davidge-Pitts, Felicity Enders, Zaraq Khan   +7 more
doaj   +1 more source

Cryopreservation of sugarcane embryogenic callus using a simplified freezing process [PDF]

CRYO-LETTERS, Volume: 19, Issue: 3, Pages: 171-176, Published: MAY-JUN 1998, Document Type:Article Published by Cryo-Letters, 7, Wootton Way, Cambridge CB3 9LX, U.K, 2020
A simplified freezing process was developed and successfully applied to embryogenic calluses of three sugarcane commercial hybrids (Saccharum sp. cv. CP 5243, C 91-301 and C 1051-73). To obtain optimal survival, the calluses were pretreated with a cryoprotective solution containing 10% DMSO and 0.3 to 0.75M sucrose.
arxiv