Results 81 to 90 of about 63,786 (247)

A digital shadow of CAR T cell expansion in a perfusion bioreactor: Informing optimal harvest times for autologous cell therapy

Biotechnology Progress, EarlyView.
Using online metabolite data from the first few days of a bioreactor run, the digital shadow can forecast the cell concentration in real‐time with an accuracy of 13% (standard deviation ≈ 6%). These predictions can guide the optimal harvest time of CAR T cells for subsequent re‐infusion into the patient.
Joseph R. Egan, Núria Marí‐Buyé, Elia Vallejo Benítez‐Cano, Miquel Costa, Linda Wanika, Michael J. Chappell, Ursula Schultz, Jelena Ochs, Manuel Effenberger, David Horna, Qasim Rafiq, Stephen Goldrick   +11 more
wiley   +1 more source

Latent class analysis of Chinese healthcare providers’ attitudes towards oocyte cryopreservation: a cross-sectional study

BMJ Open
Objectives The present study was designed to examine the attitudes towards oocyte cryopreservation among healthcare providers working in hospitals across specialties and potential influencing factors.Design A cross-sectional study.Setting The ...
Jingjing Lu, Xuezi Tian, Zhaochen Wang
doaj   +1 more source

Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach

Biotechnology Progress, EarlyView.
Abstract Clonally derived cell lines generated from Chinese hamster ovary (CHO) cells encounter numerous stressors when cultured in high‐intensity perfusion bioreactors leading to poor process performance. To circumvent this, the ability of CHO cells to adapt to different culture environments was exploited. Here host cells were selected in the presence
Peter Amaya, Rajesh K. Mistry, Susie Sou, Sonja Hess, Bijay Khanal, Zayla Schaeffer, Jie Zhu, Lina Chakrabarti   +7 more
wiley   +1 more source

Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART

Advances in Urology, 2012
Cryopreservation of human spermatozoa—introduced in the 1960's—has been recognized as an efficient procedure for management of male fertility before therapy for malignant diseases, vasectomy or surgical infertility treatments, to store donor and partner ...
Marlea Di Santo, Nicoletta Tarozzi, Marco Nadalini, Andrea Borini   +3 more
doaj   +1 more source

Fungal Biodiversity From the Atlantic Forest With Bioactive Metabolites Against Cutaneous Leishmaniasis

Chemistry &Biodiversity, EarlyView.
ABSTRACT Biodiversity offers a rich source of bioactive compounds for drug discovery. We isolated Aspergillus fumigatus from compost in the Atlantic Forest and found its extract active against Leishmania (Leishmania) amazonensis. Two crude extracts, I06 and I75, were selected for further study.
Juliana Cristina Pereira Calado, Marina Valente Navarro, Alison Felipe Alencar Chaves, Isis Casula, Patricia Locosque Ramos, João Batista da Cruz, Anderson Messias Rodrigues, Keith Dayane Leite Lira, Fernando Luiz Affonso Fonseca, Patricia Xander, Suzan Pantaroto de Vasconcellos, Wagner Luiz Batista   +11 more
wiley   +1 more source

New Insights Into Ginseng and Clove Supplementation: Alleviating Obesity by Reducing Leptin Resistance and Reshaping the Gut Microbiota

Food Frontiers, EarlyView.
AEGC conferred substantial resistance to weight gain and reduced food intake. AEGC may alleviate LR by regulating the levels of LEP and ADP. AEGC elevated the diversity of gut microbiota and reconstructed the composition. Changes such as CAG‐873 in gut microbiota may be the reason why AEGC alleviates LR.
Weifeng Ni, Shen Yao, Shichao Li, Shichao Liu, Liwei Sun, Daqing Zhao, Zhengqi Dong, Siming Wang, Weinan Wang, Meichen Liu   +9 more
wiley   +1 more source

Recent Insights on Synthesis, Stability, and Complexities of Water‐in‐Oil Pickering Emulsion: From Theory to Applications

Food Frontiers, EarlyView.
It provides a conceeptual framework of the core components required for w/o PE development and various internal and external factors determining the emulsion stabilization. It also emphasizes the emerging application of w/o PE in diverse industrial use such as encapsulation and delivery, packaging, food, biomedical, etc.
Bibha Mishra A., Vidisha Tomer
wiley   +1 more source

Altered Inflammatory Signature in a C9ORF72‐ALS iPSC‐Derived Motor Neuron and Microglia Coculture Model

Glia, EarlyView.
Established a C9ORF72 ALS microglia and motor neuron coculture model. Identified an altered inflammatory signature in C9ORF72 ALS microglia. Single‐cell RNA sequencing detected the removal of an LPS responsive microglia subpopulation. ABSTRACT Amyotrophic lateral sclerosis (ALS) is a complex neurodegenerative disorder involving multiple cell types in ...
Yujing Gao, Jessica L. Brothwood, Harpreet Saini, Gregory A. O'Sullivan, Carla F. Bento, James M. McCarthy, Nicola G. Wallis, Elena Di Daniel, Brent Graham, Daniel M. Tams   +9 more
wiley   +1 more source

Rising trends in the use of frozen dog sperm: a retrospective study in Belgium and the Netherlands

Frontiers in Veterinary Science
IntroductionSperm cryopreservation is a valuable technique for storing valuable canine genetics. However, little is known concerning the fate of frozen sperm stored in a sperm bank.
Guillaume Domain, Maarten Kappen, Amber Van Mil, Ilse De Beijer, Matthieu Van Puyvelde, Robby Van Leeuwenberg, Lotte Spanoghe, Florin Posastiuc, Ann Van Soom   +8 more
doaj   +1 more source

Somatic cell reprogramming for Parkinson's disease treatment

Ibrain, Volume 11, Issue 1, Page 59-73, Spring 2025.
The fundamental purpose of cell reprogramming to treat Parkinson's disease is to generate dopaminergic neurons (DAN) and do transplantation. There are two ways to accomplish this. One method is to induce cells into induced DA neurons (iDAN) directly or to induce cells into induced pluripotent stem cells and ultimately into iDAN in vitro. Another option
Xiaozhuo Li, Kevin Fang, Fengping Wang
wiley   +1 more source