Results 91 to 100 of about 48,476 (320)

Nature Inspired Delivery Vehicles for CRISPR‐Based Genome Editing

Small, EarlyView.
The review highlights nature‐inspired nanocarriers for CRISPR delivery, emphasizing viral vectors, extracellular vesicles, liposomes, and lipid nanoparticles. It discusses their roles in improving specificity, minimizing immunogenicity, and overcoming barriers in genome editing. Recent advancements, challenges, and therapeutic applications are explored,
Elizabeth Maria Clarissa, Mamata Karmacharya, Hyunmin Choi, Sumit Kumar, Yoon‐Kyoung Cho   +4 more
wiley   +1 more source

Stabilized Extracellular Vesicle Formulations for Inhalable Dry Powder Development

Small, EarlyView.
Extracellular vesicles (EVs) hold promise for chronic obstructive pulmonary disease (COPD) treatment but face challenges with storage and delivery. This study demonstrates the stabilization of lung fibroblast‐derived EVs using inulin, enabling freeze‐drying and spray‐drying.
Eva M. Jansen, Luke van der Koog, Robin A.B. Elferink, Karim Rafie, Anika Nagelkerke, Reinoud Gosens, Henderik W. Frijlink, Wouter L.J. Hinrichs   +7 more
wiley   +1 more source

Visualization Study on Ice Formation in Water and Dimethyl Sulfoxide Solution

Zhileng xuebao, 2022
Ice formation during freezing and rewarming processes is a key factor that affects the success rate of cell cryopreservation. The discovery of the formation and inhibition mechanism of ice crystals in various scenarios is highly significant for clinical ...
Pan Pingan, Zhang Hongbin, Huang Yonghua, Han Xia, Chen Wei, Li Zheng   +5 more
doaj  

Structure of a putative NTP pyrophosphohydrolase: YP_001813558.1 from Exiguobacterium sibiricum 255-15. [PDF]

, 2010
The crystal structure of a putative NTPase, YP_001813558.1 from Exiguobacterium sibiricum 255-15 (PF09934, DUF2166) was determined to 1.78 Å resolution. YP_001813558.1 and its homologs (dimeric dUTPases, MazG proteins and HisE-encoded phosphoribosyl ATP ...
Abdubek, Polat, Astakhova, Tamara, Axelrod, Herbert L, Carlton, Dennis, Chen, Connie, Chiu, Hsiu Ju, Clayton, Thomas, Das, Debanu, Deacon, Ashley M, Deller, Marc C, Duan, Lian, Elsliger, Marc André, Ernst, Dustin, Feuerhelm, Julie, Godzik, Adam, Grant, Joanna C, Grzechnik, Anna, Han, Gye Won, Hodgson, Keith O, Jaroszewski, Lukasz, Jin, Kevin K, Johnson, Hope A, Klock, Heath E, Knuth, Mark W, Kozbial, Piotr, Krishna, S Sri, Kumar, Abhinav, Lam, Winnie W, Lesley, Scott A, Marciano, David, McMullan, Daniel, Miller, Mitchell D, Morse, Andrew T, Murzin, Alexey G, Nigoghossian, Edward, Okach, Linda, Reyes, Ron, Rife, Christopher L, Sefcovic, Natasha, Tien, Henry J, Trame, Christine B, van den Bedem, Henry, Weekes, Dana, Wilson, Ian A, Wooley, John, Xu, Qingping, Yeates, Todd O   +46 more
core   +2 more sources

Insect cell plasma membranes do, while soluble enzymes do not, need stabilization by accumulated cryoprotectant molecules during freezing stress [PDF]

, 2022
Robert Grgac, Jan Rozsypal, Lauren E. Des Marteaux, Tomáš Štětina, Vladimı́r Košťál   +4 more
openalex   +1 more source

Cryoprotection without cryoprotectant [PDF]

Acta Crystallographica Section A Foundations and Advances, 2017
Yvonne Thielmann, David Quirnheim Pais, Barbara Rathmann, Paul Wurzinger, Cveta Tomova   +4 more
openaire   +1 more source

Mechanosensitive Cell‐Laden Shape‐Memory Scaffolds Engineered via Cryogelation

Small Structures, EarlyView.
GelMA‐based shape‐memory scaffolds encapsulating human adipose‐derived stem cells are fabricated via cryogelation, showing rapid recovery and high cell viability. Under cyclic compression, they promoted mechanotransduction gene expression, and composite formulations (collagen/GelMA, dECM/GelMA) retained robust shape‐memory, highlighting their promise ...
JaeYoon Lee, ByungJoon Choi, Hanjun Hwangbo, GaEun Heo, GeunHyung Kim   +4 more
wiley   +1 more source

Effects of cryoprotectant concentration and cooling rate on vitrification of aqueous solutions

, 2006
Vitrification of aqueous cryoprotectant mixtures is essential in cryopreservation of proteins and other biological samples. We report systematic measurements of critical cryoprotective agent (CPA) concentrations required for vitrification during plunge ...
Alsaied, Osama A., Berejnov, Viatcheslav, Husseini, Naji S., Thorne, Robert E.   +3 more
core   +1 more source

Use of methanol as cryoprotectant and its effect on sox genes and proteins in chilled zebrafish embryos [PDF]

, 2015
Methanol is a widely used cryoprotectant (CPA) in cryopreservation of fish embryos, however little is known about its effect at the molecular level. This study investigated the effect of methanol on sox gene and protein expression in zebrafish embryos ...
Ahammad, Andrews, Arav, Avilion, Crémazy, Dee, Degitz, Desai, Dinnyés, Dong, Drobnis, Eells, Emma Spikings, Ferri, Fuller, Graham, Guan, Hagedorn, Kimmel, Kishi, Kopeika, Kunjan Desai, Lahnsteiner, Lee, Lin, Liu, Mattick, Mellerick, Millimaki, Nagashima, Nageshan, Nie, Okuda, Overton, Pfaffl, Pillai, Plachinta, Rico, Shi, Smith, Tan, Tiantian Zhang, Tsai, Valdez, Vriz, Zampolla, Zeron, Zhang, Zhang, Zhang, Zhang, Zhang   +51 more
core   +3 more sources

Sperm Telomere Interactions Are Impaired in Testicular Cancer Before and After Adjuvant Therapy

Andrology, EarlyView.
ABSTRACT Background No study has yet explored telomere length or interaction in sperm nuclei of testicular cancer (TC) patients exposed to chemotherapy or radiotherapy. However, sperm telomere dynamics have emerged as a potential marker in male infertility.
Benoit Berby, Sylvianne Hennebicq, Jacqueline Saias, Florence Brugnon, Isabelle Berthaut, Myriam Daudin, Louis Bujan, Ludovic Dumont, Christine Rondanino, Aurélie Feraille, Nathalie Rives   +10 more
wiley   +1 more source