Results 191 to 200 of about 110,531 (202)
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Optimization of CUT&Tag product recovery and library construction method.
Yi chuan = Hereditas, 2021The emerging cleavage under target and tagment (CUT&Tag) technology uses Tn5 transposase to cleavage near the DNA binding site of target protein and study the generated DNA fragments by the next-generation sequencing. It can quickly identify protein-DNA interactions, which greatly simplifies the experimental process of ChIP-Seq.
Ye, Wei +3 more
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Cut-tag/ChIP-seq of AR/SP1 in prostate cancer cell lines
2021Genome binding/occupancy profiling by high throughput sequencing We examine total AR and SP1 chromatin-binding profiles in DHT-treated LNCaP and LNCaP-AI cells to address the molecular functions of SP1 on the chromatin.
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Using Cleavage Under Targets and Tagmentation (CUT&Tag) Assay in Mouse Myoblast Research.
Journal of visualized experiments : JoVEThis protocol paper aims to provide the new researchers with the full details of using Cleavage Under Targets and Tagmentation (CUT&Tag) to profile the genomic locations of chromatin binding factors, histone marks, and histone variants. CUT&Tag protocols function very well with mouse myoblasts and freshly isolated muscle stem cells (MuSCs).
Yuefeng, Li, Xiaofen, Wu, Ping, Hu
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CUT&Tag for Efficient Epigenomic Profiling of Frozen Tissues
Cleavage Under Targets and Tagmentation (CUT&Tag) provides high-resolution sequencing libraries for profiling diverse chromatin components. This protocol details the steps to generate CUT&Tag libraries from fresh or frozen tissues. This CUT&Tag workflow has nine main steps: isolation of nuclei from tissues, binding of nuclei to Concanavalin A-coated ...Qi, Yin, Yang, Li, Yuxin, Yin
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CUT&Tag for Transcription Factor Binding Profiles During Mammalian Zygotic Genome Activation
Zygotic genome activation (ZGA) triggered by transcription factors (TFs) is a critical event to facilitate subsequent embryonic development. However, the paucity of material poses a challenge for mapping TF binding profiles in mammalian embryos. Here, we describe a low-input Cleavage Under Targets & Tagmentation (CUT&Tag) protocol optimized for TF ...Kobayashi, W., Tachibana, K.
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Adaptation of the CUT&Tag protocol to bacteria
CUT&Tag is a molecular biology method that combines protein A-Tn5 fusion-mediated targeted cleavage with DNA sequencing. This method identifies binding sites for DNA-associated proteins. While the method is developed for eukaryotes, a protocol is lacking for bacteria.openaire +1 more source