Partial purification of cytidine deaminase from protoscoleces of Echinococcus granulosus [PDF]
Kirkuk Journal of Science, 2015 Cytidine deaminase (EC 3.5.4.5) has been partially purified from protoscoleces of Echinococcus granulosus by gel filteration chromatography using Sephadex G200 .The molecular weight of the enzyme determined by gel filteration was about 82000 dalton with ...
Husain F. Hassan, Jenan A.Saber
doaj +1 more source
Rational Design of RNA Editing Guide Strands: Cytidine Analogs at the Orphan Position.
Journal of the American Chemical Society, 2021 Adenosine Deaminases Acting on RNA (ADARs) convert adenosine to inosine in double stranded RNA. Human ADARs can be directed to predetermined target sites in the transcriptome by complementary guide strands, allowing for the correction of disease-causing ...
Erin E. Doherty +8 more
semanticscholar +1 more source
Enhanced mitochondrial activity reshapes a gut microbiota profile that delays NASH progression
Hepatology, EarlyView., 2022 Improved mitochondrial activity, due to the lack of methylation‐controlled J protein (MCJ), creates a specific microbiota signature that when transferred through cecal microbiota transplantation delays NASH progression by restoring the gut‐liver axis and enhancing hepatic fatty acid oxidation.
María Juárez‐Fernández +18 more
wiley +1 more source
Determination of total potentially available nucleosides in bovine milk [PDF]
, 2010 Bovine colostrum and milk samples were collected from two herds over the course of the first month post-partum, pooled for each herd by stage of lactation and total potentially available nucleosides were determined.
Gill, Brendon D. +2 more
core +2 more sources
Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction
Nature Biotechnology, 2018 Base editors enable targeted single-nucleotide conversions in genomic DNA. Here we show that expression levels are a bottleneck in base-editing efficiency.
Luke W. Koblan +8 more
semanticscholar +1 more source
Transcription as a Threat to Genome Integrity [PDF]
, 2016 Genomes undergo different types of sporadic alterations, including DNA damage, point mutations, and genome rearrangements, that constitute the basis for evolution.
Aguilera López, Andrés +1 more
core +1 more source
Natural variation in Vif: differential impact on APOBEC3G/3F and a potential role in HIV-1 diversification. [PDF]
PLoS Pathogens, 2005 The HIV-1 Vif protein counteracts the antiviral activity exhibited by the host cytidine deaminases APOBEC3G and APOBEC3F. Here, we show that defective vif alleles can readily be found in HIV-1 isolates and infected patients. Single residue changes in the
Viviana Simon +5 more
doaj +2 more sources
Nature Communications, 2023 APOBEC’s are a family of cytidine deaminases that induce mutations in viruses to inhibit their replication and maintain cell integrity. Here, Manjunath et al show that APOBEC3B also inhibits viral replication by stimulating the innate immune sensor ...
Lavanya Manjunath +12 more
doaj +1 more source
Highly Efficient CRISPR-Mediated Base Editing in Sinorhizobium meliloti
Frontiers in Microbiology, 2021 Rhizobia are widespread gram-negative soil bacteria and indispensable symbiotic partners of leguminous plants that facilitate the most highly efficient biological nitrogen fixation in nature.
Longxiang Wang +6 more
doaj +1 more source
Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. [PDF]
, 2017 Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable "base editing" system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase,
Bai, Haipeng +7 more
core +1 more source