Results 61 to 70 of about 18,368 (174)

Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements

Nature Communications, 2019
Lineage tracing has provided new insights into cell fate but defining cellular diversity remains a challenge. Here the authors target endogenous repeat regions in mammalian cells with cytidine deaminase fused to nCas9 to create genetic barcodes for fine ...
Byungjin Hwang, Wookjae Lee, Soo-Young Yum, Yujin Jeon, Namjin Cho, Goo Jang, Duhee Bang   +6 more
doaj   +1 more source

AI foundation models in plant biology

New Phytologist, EarlyView.
Foundation models decode genomes, engineer proteins, phenotype crops, and drive AI agents across plant biology. This figure was created in BioRender (BioRender.com/xcmmkel). Summary Rapid technological progress has enabled plant biologists to accumulate unprecedented volumes of multi‐scale, multi‐modal data, yet this abundance of data has intensified ...
Haopeng Yu
wiley   +1 more source

Method of treating disorders characterized by overexpression of cytidine deaminase or deoxycytidine deaminase

, 2002
A composition, device, kit and method for countering or diagnosing cytidine deaminase or deoxycytidine deaminase over-expression or a disorder associated with it, or an increase in number or toxicity of pro-inflammatory cells that over-express cytidine ...
Nyce, Jonathan W.
core   +1 more source

Impact of DNA ligase IV on nonhomologous end joining pathways during class switch recombination in human cells

, 2005
Class switch recombination (CSR) is a region-specific, transcriptionally regulated, nonhomologous recombinational process that is initiated by activation-induced cytidine deaminase (AID).
Lennart Hammarström, Hammarström, L,, Lahdesmaki, A, Aleksi Lähdesmäki, Wei Zhou, Andrew R. Gennery, Jones, AM, Michael R. Ehrenstein, Pan-Hammarstrom, Q, Hammarstrom, L, Zhou, W, Gennery, AR, Ehrenstein, MR, Anne-Marie Jones, Richard A. Gatti, Gatti, RA, Qiang Pan-Hammarström   +16 more
core   +1 more source

Induction of homologous recombination between sequence repeats by the activation induced cytidine deaminase (AID) protein

eLife, 2014
The activation induced cytidine deaminase (AID) protein is known to initiate somatic hypermutation, gene conversion or switch recombination by cytidine deamination within the immunoglobulin loci.
Jean-Marie Buerstedde, Noel Lowndes, David G Schatz   +2 more
doaj   +1 more source

Acute pain transfusion reaction in a patient with thalassemia: In‐depth characterization of short‐ and long‐term phenotypes

Transfusion, EarlyView.
Abstract Background Acute pain transfusion reaction (APTR) is a rare, under‐recognized condition of unknown etiology. It can cause significant distress in recipients, necessitating symptomatic management and, occasionally, hospitalization. Study Design and Methods Here, we present an APTR event in an adult subject with transfusion‐dependent thalassemia
Georgia Tzafa, Sophia Delicou, Ioanna Barla, Konstantina Theocharaki, Aggeliki Kondi, Athanassios D. Velentzas, Nikolaos Simantiris, Elli‐Anna Stylianaki, George Mpekoulis, Efthymia Pavlou, Niki Vassilaki, Ioannis V. Kostopoulos, Aikaterini Xydaki, Efrosyni Nomikou, Ioanna‐Katerina Aggeli, Nikolaos Thomaidis, Evangelos Gikas, Martina Samiotaki, Jerome Zoidakis, Constantina Politis, Marianna H. Antonelou   +20 more
wiley   +1 more source

Double-stranded DNA deaminase DddAE1347A can increase the efficiency and targeting range of cytidine base editors

Genome Biology
Background Cytidine base editors (CBEs) consist of a single-strand specific cytidine deaminase fused to Cas9 nickase, enabling efficient C-to-T conversion across diverse organisms.
Yuqiang Qian, Fengjiao Hui, Wenchao Niu, Di Wang, Yang Hao, Qingying Meng, Siyu Ren, Deqiang Kong, Heng Gong, Jiayu Wu, Kexin Chen, Muna Alariqi, Junping Gao, Zhanjun Li, Shuangxia Jin   +14 more
doaj   +1 more source

SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds

Genome Biology, 2020
We describe here a CRISPR simultaneous and wide-editing induced by a single system (SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase and adenosine deaminase to Cas9 nickase ...
Chao Li, Yuan Zong, Shuai Jin, Haocheng Zhu, Dexing Lin, Shengnan Li, Jin-Long Qiu, Yanpeng Wang, Caixia Gao   +8 more
doaj   +1 more source

High‐density mutation tracks are associated with proton‐beam irradiation patterns in Sorghum bicolor

The Plant Genome, Volume 19, Issue 3, September 2026.
Abstract Induced mutagenesis is a cornerstone of crop functional genomics, yet the extent to which distinct radiation sources reshape the spatial distribution of mutations remains difficult to evaluate in reduced‐representation datasets. Here, we analyze a published genotyping‐by‐sequencing (GBS) panel (192,040 loci) to compare proton‐beam and gamma ...
Ezekiel Ahn, Insuck Baek, Seunghyun Lim, Louis K. Prom, Moon S. Kim, Lyndel W. Meinhardt, Clint Magill   +6 more
wiley   +1 more source

BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B

BMC Biology, 2018
Background Base Editing is a precise genome editing method that uses a deaminase-Cas9 fusion protein to mutate cytidine to thymidine in target DNA in situ without the generation of a double-strand break.
Matthew A. Coelho, Songyuan Li, Luna Simona Pane, Mike Firth, Giovanni Ciotta, Jonathan D. Wrigley, Maria Emanuela Cuomo, Marcello Maresca, Benjamin J. M. Taylor   +8 more
doaj   +1 more source