Results 71 to 80 of about 38,369 (222)

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

Cytidine deaminase activity in synovial fluid of patients with rheumatoid arthritis: relation to lactoferrin, acidosis, and cartilage proteoglycan release

, 1990
It is claimed that cytidine deaminase activity reflects local granulocyte turnover or activity in the synovial fluid of patients with rheumatoid arthritis, but cytidine deaminase is not a granulocyte specific enzyme. Lactoferrin is a granulocyte specific
Geborek, Pierre, Björnsson, Sven, Saxne, Tore, Månsson, Bengt   +3 more
core   +2 more sources

Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements

Nature Communications, 2019
Lineage tracing has provided new insights into cell fate but defining cellular diversity remains a challenge. Here the authors target endogenous repeat regions in mammalian cells with cytidine deaminase fused to nCas9 to create genetic barcodes for fine ...
Byungjin Hwang, Wookjae Lee, Soo-Young Yum, Yujin Jeon, Namjin Cho, Goo Jang, Duhee Bang   +6 more
doaj   +1 more source

Epigenetic targeting of activation-induced cytidine deaminase [PDF]

Proceedings of the National Academy of Sciences, 2014
Significance Activation-induced cytidine deaminase (AID) is a DNA modifying enzyme crucial for the generation of efficacious antibodies. AID also promiscuously introduces DNA lesions at cancer genes, leading to their chromosome translocation and lymphoma. However, how AID is recruited to these off targets is not well understood. Here,
Qiao, Wang, Thiago, Oliveira, Mila, Jankovic, Israel T, Silva, Ofir, Hakim, Kaihui, Yao, Anna, Gazumyan, Christian T, Mayer, Rushad, Pavri, Rafael, Casellas, Michel C, Nussenzweig, Davide F, Robbiani   +11 more
openaire   +2 more sources

Machine Learning‐Derived Immune Gene Signature Predicts Prognosis and Therapeutic Vulnerabilities in Multiple Myeloma

Cancer Science, EarlyView.
An immune‐related 12‐gene signature developed through integrative machine learning stratified overall survival across multiple myeloma cohorts and remained independently associated with outcome. Single‐cell, somatic mutation, and transcriptome‐based drug‐response analyses further linked the signature to multicellular bone marrow context, distinct ...
Kai Wang, Chenfei Zhao, Jingru Shi, Shiwei Liu, Yi Chen, Jujuan Wang, Zhengxu Sun, Sanmei Wang, Lei Fan, Jin Fan, Xiaoyan Qu   +10 more
wiley   +1 more source

Supplementary Tables 1-2 from Gemcitabine Pharmacogenomics: Cytidine Deaminase and Deoxycytidylate Deaminase Gene Resequencing and Functional Genomics

, 2006
Supplementary Tables 1-2 from Gemcitabine Pharmacogenomics: Cytidine Deaminase and Deoxycytidylate Deaminase Gene Resequencing and Functional ...
Judith A. Gilbert (14980864), Richard M. Weinshilboum (7375184), Eric D. Wieben (11502282), Matthew M. Ames (8863124), Yuan Ji (5702), Bruce W. Eckloff (14875755), Oreste E. Salavaggione (14875746), Linda L. Pelleymounter (14897828)   +7 more
core   +1 more source

Human APOBEC3G suppresses homologous recombination and LIG4‐independent end joining in DNA double‐strand break repair

The FEBS Journal, EarlyView.
Chromosomal DNA double‐strand breaks (DSBs) are repaired by homologous recombination and nonhomologous end joining (NHEJ). Recent work has additionally established theta‐mediated end‐joining (TMEJ) as a mechanism for DSB joining. Cells lacking NHEJ and TMEJ can repair DSBs in a homology‐dependent manner.
Shinta Saito, Miori Murayama, Usaki Arai, Sekifumi Akatsu, Ami Yamamoto, Misaki Kadoguchi, Noritaka Adachi   +6 more
wiley   +1 more source

Double-stranded DNA deaminase DddAE1347A can increase the efficiency and targeting range of cytidine base editors

Genome Biology
Background Cytidine base editors (CBEs) consist of a single-strand specific cytidine deaminase fused to Cas9 nickase, enabling efficient C-to-T conversion across diverse organisms.
Yuqiang Qian, Fengjiao Hui, Wenchao Niu, Di Wang, Yang Hao, Qingying Meng, Siyu Ren, Deqiang Kong, Heng Gong, Jiayu Wu, Kexin Chen, Muna Alariqi, Junping Gao, Zhanjun Li, Shuangxia Jin   +14 more
doaj   +1 more source

Induction of homologous recombination between sequence repeats by the activation induced cytidine deaminase (AID) protein

eLife, 2014
The activation induced cytidine deaminase (AID) protein is known to initiate somatic hypermutation, gene conversion or switch recombination by cytidine deamination within the immunoglobulin loci.
Jean-Marie Buerstedde, Noel Lowndes, David G Schatz   +2 more
doaj   +1 more source

APOBEC3s: DNA‐editing human cytidine deaminases [PDF]

Protein Science, 2019
AbstractNucleic acid editing enzymes are essential components of the human immune system that lethally mutate viral pathogens and somatically mutate immunoglobulins. Among these enzymes are cytidine deaminases of the apolipoprotein B mRNA editing enzyme, catalytic polypeptide‐like (APOBEC) super family, each with unique target sequence specificity and ...
Tania V. Silvas, Celia A. Schiffer
openaire   +3 more sources