Results 1 to 10 of about 2,416 (164)

Purification and characterization of the nuclear cytidine 5'-monophosphate N-acetylneuraminic acid synthetase from rat liver.

Journal of Biological Chemistry, 1992
N-Acetylneuraminic acid cytidylyltransferase (EC 2.7.7.43) (CAMP-NeuAc synthetase) from rat liver catalyzes the formation of cytidine monophosphate N-acetylneuraminic acid from CTP and NeuAc. We have purified this enzyme to apparent homogeneity (241-fold) using gel filtration on Sephacryl S-200 and two types of affinity chromatographies (Reactive Brown-
L B, Rodríguez-Aparicio, J M, Luengo, C, González-Clemente, A, Reglero   +3 more
openaire   +3 more sources

Mouse liver cytidine‐5′‐monophosphate‐N‐acetylneuraminic acid hydroxylase

European Journal of Biochemistry, 1992
In this paper, we present the results of an investigation into the catalytic properties of CMP‐Neu5Ac hydroxylase (Neu5Ac: N‐acetylneuraminic acid) in high‐speed supernatants of mouse liver. The enzyme was most active in Hepes/NaOH pH 7.4 and was markedly inhibited by relatively small increases in ionic strength, though the inhibition was abolished by ...
L, Shaw, P, Schneckenburger, J, Carlsen, K, Christiansen, R, Schauer   +4 more
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Biocompatibility of Genetically-Engineered Pig Cornea in Corneal Xenotransplantation: Preliminary In Vitro Study. [PDF]

Xenotransplantation
ABSTRACT Introduction We aimed to evaluate biophysical compatibility of corneal endothelial cells in genetically‐engineered (GE) pigs to identify the best candidate for in vivo nonhuman primate (NHP) study and to collect baseline data for future clinical trials from a biological function perspective.
Lee SH, Kim CY, Ryu JS, Choi DH, Yoon CH, Park CG, Choi K, Kim H, Kim MK.   +8 more
europepmc   +2 more sources

Production and Purification of Secretory Simian Cytidine Monophosphate-<i>N</i>-acetylneuraminic Acid Hydroxylase Using Baculovirus-Protein Expression System

Biological & Pharmaceutical Bulletin, 2015
Cytidine monophosphate (CMP) N-acetylneuraminic acid (Neu5Ac) hydroxylase (CMAH) is an essential enzyme for N-glycolylneuraminic acid (Neu5Gc) synthesis. In humans, Neu5Gc cannot be synthesized because of a deletion in the CMAH gene. Since Neu5Gc research has not been actively performed in comparison with Neu5Ac research, little is known about the ...
Tadanobu, Takahashi, Sawako, Kawagishi, Hiroki, Funahashi, Nonoka, Hayashi, Takashi, Suzuki   +4 more
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Unraveling the impact of sialic acids on the immune landscape and immunotherapy efficacy in pancreatic cancer [PDF]

Journal for ImmunoTherapy of Cancer, 2023
Background Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers. Despite the successful application of immune checkpoint blockade in a range of human cancers, immunotherapy in PDAC remains unsuccessful.
Manfred Wuhrer, Louis Boon, Di Wang, Yvette van Kooyk, Ernesto Rodriguez, Joke M M Den Haan, Nadine van Montfoort, Sandra J van Vliet, Kelly Boelaars, Laura Goossens-Kruijssen, Charlotte M de Winde, Dimitri Lindijer, Babet Springer, Irene van der Haar Àvila, Aram de Haas, Laetitia Wehry, Reina E Mebius   +16 more
doaj   +3 more sources

Purification, cloning, and expression of a cytidine 5'-monophosphate N-acetylneuraminic acid synthetase from Haemophilus ducreyi.

The Journal of biological chemistry, 1996
An N-acetylneuraminic acid cytidylyltransferase (EC 2.7.7.43) (CMP-NeuAc synthetase) was isolated from a Haemophilus ducreyi strain 35000 cell lysate and partially characterized. The enzyme catalyzes the reaction of CTP and NeuAc to form CMP-NeuAc, which is the nucleotide sugar donor used by sialyltransferases.
M V, Tullius, R S, Munson, J, Wang, B W, Gibson   +3 more
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Chemoenzymatically Synthesized O-Acetylated GD3 Gangliosides to Examine Viral Receptor Specificities in a Cellular Context. [PDF]

Angew Chem Int Ed Engl
7‐O‐, 9‐O‐, and 7,9‐di‐O‐acetylated GD3 gangliosides can be prepared by chemical assembly of a tetrasaccharide as α‐glycosyl fluoride that is coupled to sphingosine using a glycosynthase, followed by O‐acetyl editing by coronaviral hemagglutinin‐esterases (HEs).
Zhang Z, Liang R, Hooijschuur KC, de Vries RP, Li Z, Boons GJ.   +5 more
europepmc   +3 more sources

Reversible Sialylation:  Synthesis of Cytidine 5‘-Monophospho-N-acetylneuraminic Acid from Cytidine 5‘-Monophosphate with α2,3-Sialyl O-Glycan-, Glycolipid-, and Macromolecule-Based Donors Yields Diverse Sialylated Products^† [PDF]

Biochemistry, 2007
Sialyltransferases transfer sialic acid from cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuAc) to an acceptor molecule. Trans-sialidases of parasites transfer alpha2,3-linked sialic acid from one molecule to another without the involvement of CMP-NeuAc.
E V, Chandrasekaran, Jun, Xue, Jie, Xia, Robert D, Locke, Khushi L, Matta, Sriram, Neelamegham   +5 more
openaire   +3 more sources

CD46 is a cellular receptor for species D human adenovirus [PDF]

mBio
Human adenovirus species D (HAdV-D) contains two-thirds of all known HAdV types (116 in total) and is important as a vector in clinical applications. However, the broad tropism exhibited by several HAdV-D types poses challenges for their use as targeted ...
Katarina Danskog, Fredrik Petersen, Lars Frängsmyr, Gabriel Gonzalez, Miriam Becker, Annasara Lenman, Niklas Arnberg   +6 more
doaj   +2 more sources

The First Clinical Renal Xenotransplantation Study (EXPAND): Evaluating Safety, Function, and Zoonotic Risks. [PDF]

Health Care Sci
Health Care Science, Volume 4, Issue 6, Page 377-380, December 2025.
Nashan B.
europepmc   +2 more sources