Results 71 to 80 of about 9,325 (207)

Cytometry‐based single‐cell analysis of intact epithelial signaling reveals MAPK activation divergent from TNF‐α‐induced apoptosis in vivo

Molecular Systems Biology, 2015
Understanding heterogeneous cellular behaviors in a complex tissue requires the evaluation of signaling networks at single‐cell resolution. However, probing signaling in epithelial tissues using cytometry‐based single‐cell analysis has been confounded by
Alan J Simmons, Amrita Banerjee, Eliot T McKinley, Cherie' R Scurrah, Charles A Herring, Leslie S Gewin, Ryota Masuzaki, Seth J Karp, Jeffrey L Franklin, Michael J Gerdes, Jonathan M Irish, Robert J Coffey, Ken S Lau   +12 more
doaj   +1 more source

Mass cytometry and single-cell RNA sequencing reveal immune cell characteristics of active and inactive phases of Crohn’s disease

Frontiers in Medicine, 2023
ObjectivesFor Crohn’s disease (CD), the alternation of the active phase and inactive phase may be related to humoral immunity and cellular immunity. This study aims to understand the characteristics of immune cells in patients with active CD (CDa) and ...
Wenjia Lin, Shiying Liu, Zhuojian Huang, Haiwen Li, Tianyu Lu, Yongxin Luo, Jiamin Zhong, Zewen Xu, Yu Liu, Yanwu Li, Yanwu Li, Peiwu Li, Qian Xu, Jiazhong Cai, Jiazhong Cai, Huibiao Li, Xin-lin Chen   +16 more
doaj   +1 more source

Artificial Intelligence for Identifying Tumor‐Reactive CD8+ T Cells: Biological Principles, Computational Advances, and Future Directions

Med Research, EarlyView.
This review details a three‐stage paradigm shift for tumor‐reactive CD8+ T‐cell identification: decoding transcriptomic states, deciphering clonal functional efficacy, and molecular‐level therapeutic TCR design. Addressing translational hurdles and generative AI “scientific blind spots”—such as missing catch bonds—we present a visionary roadmap.
Chao Yang, Yingying Sun, Yan Zhang, Yifen Shi, Zhiwen Shi   +4 more
wiley   +1 more source

Integration of mass cytometry and single-cell RNA-sequencing of cells in bronchoalveolar lavage

BioTechniques
Single-cell RNA sequencing (sc-RNA-seq) is a popular method for characterization of cell populations. However, the relationship between RNA and protein expression in cells is often discordant. Protein-based detection methods, such as cytometry by time-of-
Elizabeth Guinto, Sameeksha Chopra, I-Chih Kuo, Samuel B. Shin, Melina Messing, Chung Y. Cheung, Julia SW. Yang, Firoozeh V. Gerayeli, William Yip, Stephen Milne, Rachel L. Eddy, Janice M. Leung, Kelly M. McNagny, Don D. Sin   +13 more
doaj   +1 more source

Mass cytometry reveals the corneal immune cell changes at single cell level in diabetic mice

Frontiers in Endocrinology, 2023
IntroductionDiabetic ocular complications include sight-threatening consequences and decreased corneal sensitivity, characterized by decreased tear production, corneal sensitivity and delayed corneal epithelial wound healing. The pathogenesis of diabetic
Limin Qin, Limin Qin, Limin Qin, Qian Li, Qian Li, Qian Li, Liqiang Wang, Yifei Huang   +7 more
doaj   +1 more source

Four‐dimensional immuno‐mechanobiomaterials: Dynamic material cues that program host response to prevent fibrosis and enable regeneration

VIEW, EarlyView.
Dynamic immuno‐mechanobiomaterials with time‐programmed mechanical adaptation orchestrate host responses through precise mechanotransduction and immune‐stromal regulation. These adaptive biomaterials minimize mechanical mismatch, attenuate the forign dy response and fibrosis, promote angiogenesis and controlled extracellular matrix remodeling, and ...
Gobinath Vellapalayam Manoharan, Kathirvel Ayyaswamy, Parthasarathy Vellaichamy, Nagarajan Deivanayagam Pillai, Rithik Gobinath   +4 more
wiley   +1 more source

In vivo single-cell high-dimensional mass cytometry analysis to track the interactions between Klebsiella pneumoniae and myeloid cells.

PLoS Pathogens
In vivo single-cell approaches have transformed our understanding of the immune populations in tissues. Mass cytometry (CyTOF), that combines the resolution of mass spectrometry with the ability to conduct multiplexed measurements of cell molecules at ...
Ricardo Calderon-Gonzalez, Amy Dumigan, Joana Sá-Pessoa, Adrien Kissenpfennig, José A Bengoechea   +4 more
doaj   +1 more source

Meeting the Challenges of High-Dimensional Single-Cell Data Analysis in Immunology

Frontiers in Immunology, 2019
Recent advances in cytometry have radically altered the fate of single-cell proteomics by allowing a more accurate understanding of complex biological systems.
Subarna Palit, Subarna Palit, Subarna Palit, Christoph Heuser, Christoph Heuser, Christoph Heuser, Gustavo P. de Almeida, Gustavo P. de Almeida, Gustavo P. de Almeida, Fabian J. Theis, Fabian J. Theis, Christina E. Zielinski, Christina E. Zielinski, Christina E. Zielinski   +13 more
doaj   +1 more source

Lupus Panniculitis Immunoprofiling Reveals a Cytotoxic, Type 1‐Skewed T‐Cell Response With Signs of Metabolic Alteration

Allergy, EarlyView.
Imaging mass cytometry shows lupus panniculitis lesions are dominated by cytotoxic and skin‐homing T cells with abundant macrophages and B‐cell aggregates. Spatial neighborhood analysis reveals organized leukocyte architecture, including B‐cell proximity to naïve T cells and M1 macrophages to cytotoxic T cells.
Milad Ameri, Reihane Ziadlou, Stephan Traidl, Milena Sokolowska, Emmanuel Contassot, Nick Li, Maija Kiuru, Isabel Kolm, Werner Kempf, Emanual Maverakis, Cezmi Akdis, Marie‐Charlotte Brüggen   +11 more
wiley   +1 more source

MOESM2 of Baseline immune profile by CyTOF can predict response to an investigational adjuvanted vaccine in elderly adults

, 2018
Additional file 2.
Stacie Lambert (5338328), Holden Maecker (33297), Nithya Swaminathan (5338325), Sangeeta Kowli (5338331), Christine Lingblom (5338334)   +4 more
core   +1 more source