Results 91 to 100 of about 15,403 (255)

High expression of uracil DNA glycosylase determines C to T substitution in human pluripotent stem cells

Molecular Therapy: Nucleic Acids, 2022
Precise genome editing of human pluripotent stem cells (hPSCs) is crucial not only for basic science but also for biomedical applications such as ex vivo stem cell therapy and genetic disease modeling.
Ju-Chan Park, Hyeon-Ki Jang, Jumee Kim, Jun Hee Han, Youngri Jung, Keuntae Kim, Sangsu Bae, Hyuk-Jin Cha   +7 more
doaj   +1 more source

Ligand‐Defined Interfacial Chemistry Enables Instant and Sequence‐General DNA Chemisorption on Gold Nanoparticles Toward Label‐Free SERS With Single‐Base Resolution

Advanced Science, EarlyView.
Ascorbic Acid‐Engineered Interface Enables Universal DNA Functionalization on Gold Nanoparticles: Attenuating electrostatic repulsion and leveraging synergistic hydrogen‐bonding interactions significantly lowers the kinetic barrier for DNA adsorption, enabling rapid and sequence‐general functionalization of non‐thiolated DNA‐requiring only a single ...
Guangping Li, Cheng Wang, Xinyue Wu, Zhongxiang Ding, Heng Gao, Zhonggang Liu, Yu Zhang, Jinai Chen, Feng Wang, Honglin Liu   +9 more
wiley   +1 more source

Precise base editing of non-allelic acetolactate synthase genes confers sulfonylurea herbicide resistance in maize

Crop Journal, 2020
Single-nucleotide polymorphisms contribute to phenotypic diversity in maize. Creation and functional annotation of point mutations has been limited by the low efficiency of conventional methods based on random mutation.
Yanmin Li, Jinjie Zhu, Hao Wu, Changlin Liu, Changling Huang, Jinhao Lan, Yanming Zhao, Chuanxiao Xie   +7 more
doaj   +1 more source

Targeting Dnmt3a/m5C/RelA Axis Attenuates Microglia Inflammatory Response and Improves Postoperative Recovery in Chronic Compressive Cervical Spinal Cord Injury

Advanced Science, EarlyView.
A pro‐inflammatory microglial subset persists after decompression in chronic compressive cervical spinal cord injury and shows enhanced Dnmt3a‐driven m5C signaling. By stabilizing RelA mRNA, this axis sustains NF‐κB activation and postoperative neuroinflammation.
Tianyu Qin, Yuan Jiang, Yongheng Xie, Huanwei Qu, Wenbin Yan, Jingle Chen, Yining Chen, Huayi Li, Naibo Feng, Jiajun Wu, Chao Zhang, Zhengqi Huang, Ming Shi, Zhihuai Deng, Guozhi Xiao, Houqing Long   +15 more
wiley   +1 more source

Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors

Nature Biotechnology, 2020
Cytosine base editors (CBEs) enable targeted C•G-to-T•A conversions in genomic DNA. Recent studies report that BE3, the original CBE, induces a low frequency of genome-wide Cas9-independent off-target C•G-to-T•A mutation in mouse embryos and in rice. Here we develop multiple rapid, cost-effective methods to screen the propensity of different CBEs to ...
Jordan L. Doman, Aditya Raguram, Gregory A. Newby, David R. Liu   +3 more
openaire   +5 more sources

Synergistic HMGN1 and VP64 Fusions Potentiate High‐Precision and PAM‐Flexible Base Editing

Advanced Science, EarlyView.
A novel CDA1Δ‐SpRY architecture fused with HMGN1 and VP64 yields a nearly PAM‐less base editing platform. By focusing cytosine conversion predominantly at position −18, this synergistic complex ensures highly precise targeting. Demonstrating enhanced efficiency across diverse models, including yeast and rice, the platform offers a robust solution for ...
Xi Luo, Yuyao Qu, Zhengyan Ye, Zheng Li, Yuanyan Zhang, Longjiong Luo, Shaokang Li, Wei Zhao, Ming Wang, Ralph Bock, Jianmin Wan, Junjie Tan   +11 more
wiley   +1 more source

Efficient and Safe Editing of Porcine Endogenous Retrovirus Genomes by Multiple-Site Base-Editing Editor

, 2022
Gene-modified miniature pigs serve as alternative tissue and organ donors for xenotransplantation to alleviate the shortage of human allogenic organs.
Wansheng Li, Zhong, Haiwen, Zhou, Xiaoqing, Li, Wansheng, Zi, Yin, Wang, Jinling, Yin Zi, Wei Zheng, Chi, Yue, Tang, Chengcheng, Liangxue Lai, Lai, Liangxue, Xiaoqing Zhou, Chengcheng Tang, Jinling Wang, Shuwen Zheng, Qingjian Zou, Zheng, Wei, Zou, Qingjian, Chen, Min, Haiwen Zhong, Min Chen, Yue Chi, Zheng, Shuwen   +23 more
core   +1 more source

Comparison of cytosine base editors and development of the BEable-GPS database for targeting pathogenic SNVs [PDF]

Genome Biology, 2019
Abstract A variety of base editors have been developed to achieve C-to-T editing in different genomic contexts. Here, we compare a panel of five base editors on their C-to-T editing efficiencies and product purity at commonly editable sites, including some human pathogenic C-to-T mutations.
Ying Wang, Runze Gao, Jing Wu, Yi-Chun Xiong, Jia Wei, Sipin Zhang, Bei Yang, Jia Chen, Li Yang   +8 more
openaire   +3 more sources

Nano‐Backpack Engineered Probiotics with Reactive Oxygen Species–Responsive Tungsten Release for Synergistic Therapy of Inflammatory Bowel Disease

Advanced Science, EarlyView.
An integrated probiotic system is engineered to execute a programmed therapeutic cascade against inflammatory bowel disease. Upon pathological signals, the system sequentially performs ROS scavenging, tungsten release, and selective pathogen suppression, thereby breaking the oxidative stress–dysbiosis cycle to remodel gut microbiota and reinforce ...
Yang Yang, Liucan Wang, Guoqing Chen, Yuanling Zhang, Jiarui Shi, Wenzhe Fan, Min Yu, Jixi Zhang, Hua Yang   +8 more
wiley   +1 more source

Research progress of base editing and prime editing tools based on the CRISPR/Cas system

Molecular Therapy: Nucleic Acids
The base editor (BE) and prime editing guide RNA (pegRNA)-based prime editor (PE) technologies relying on the CRISPR/Cas system are very efficient gene editors that have been developed in recent years.
Herui Gao, Song Gao, Guoshi Kan, Leonid N. Valentovich, Yingfeng An   +4 more
doaj   +1 more source